Skip to main content
NCBI home page
Chronic Diseases and Translational Medicine logoLink to Chronic Diseases and Translational Medicine
. 2020 Jan 8;5(4):243–251. doi: 10.1016/j.cdtm.2019.12.003

Diagnosis and treatment of Helicobacter pylori infections in children and elderly populations

Chao Peng a,d, Yi Hu a,d, Zhong-Ming Ge b, Quan-Ming Zou c,∗∗, Nong-Hua Lyu a,∗
PMCID: PMC7005112  PMID: 32055783

Abstract

Helicobacter pylori (H. pylori) infection is associated with various gastric and extra-gastric diseases. Importantly, this infection is the strongest known risk factor for gastric cancer (GC). H. pylori eradication can effectively prevent H. pylori infection-associated diseases in H. pylori-positive patients, including children and elderly subjects. However, a limited selection of antibiotics, a higher reinfection rate, and certain spontaneous clearance rates, to some extent, restrict the choice of H. pylori treatments in pediatrics. In addition, it is imperative to perform an accurate diagnosis of H. pylori infection in children by determining the presence of the H. pylori infection and the underlying cause of symptoms. In elderly patients, poor tolerance to drugs and higher sensitivity to adverse effects are major concerns during H. pylori therapy. Recent studies have demonstrated that H. pylori eradication could significantly lower the GC risk in the elderly population. The benefit and risk of H. pylori eradication in elderly patients should be comprehensively considered and balanced. If available, susceptibility-based tailored therapies may be preferable in eradicating H. pylori. In addition, to increase the eradication rate and reduce adverse effects, new therapeutic strategies (e.g., probiotic supplementation, berberine supplementation, dual therapy) for H. pylori infection are being extensively investigated. The impact of H. pylori eradication with antibiotics on the microbiota in children has been explored, but further high-quality studies are crucial to delineate the extent of H. pylori eradication affecting the microbial community in children. In this review, we summarize the current understanding of H. pylori diagnosis and treatment in children and the elderly population and aim to provide insights into the efficient management and treatment implementation in these populations.

Keywords: Children, Elderly, Helicobacter pylori, Diagnosis, Treatment

Introduction

Helicobacter pylori (H. pylori), a Gram-negative bacterium that colonizes the mucosa of the human stomach and infects over half the world's population, can induce chronic gastritis, peptic ulcers, and gastric mucosa-associated lymphoid tissue lymphoma.1 The International Agency for the Research on Cancer (IARC) classified H. pylori as a Group 1 carcinogen in 1994.2 H. pylori infection is the strongest risk factor for gastric cancer (GC), with at least 90% of GC attributed to H. pylori infection.3 H. pylori-induced gastritis has been defined as an infectious disease in multiple guidelines.4, 5, 6 The routes of H. pylori transmission are considered to be oral–oral, fecal-oral, or gastro-oral.7 Furthermore, H. pylori prevalence varies in different geographic areas. High H. pylori prevalence is commonly considered to be associated with poor sanitary conditions and a low socioeconomic status. A meta-analysis indicated that among individual countries, the prevalence of H. pylori infection varied from as low as 18.9% in Switzerland to 87.7% in Nigeria.1 The H. pylori prevalence remained high in most countries despite a decreasing trend observed for H. pylori infection rates in some developed countries.1,8,9 Successful H. pylori eradication can effectively prevent H. pylori-associated diseases. The efficacy of standard triple therapy has been significantly decreased owing to increased antibiotic resistance.10 Hence, the efficacy of novel regimens (e.g., concomitant therapy, sequential therapy, bismuth-quadruple therapy) has been extensively investigated in eradicating H. pylori.

Advanced age is an important risk factor for chronic gastritis and GC.11,12 However, H. pylori infection can be primarily acquired during infancy and increases with aging.13 The Kyoto consensus on H. pylori gastritis firstly proposed that H. pylori-infected individuals should be offered eradication therapy unless there are competing considerations.5 Therefore, H. pylori-infected children and elderly patients are also included in the patient populations who require H. pylori treatment. The World Health Organization has defined people older than 60 years as elderly. People under 18 years are defined as children according to the “UN Convention on the Rights of the Child”. However, there are still considerable controversies in clinical practice regarding the management of H. pylori infections in children and the elderly.

Considering the poor tolerance to drugs, high sensitivity to adverse effects, relatively poor compliance, and antibiotic resistance, the management of H. pylori in children and elderly patients necessitates additional considerations than that in adult patients. Initial treatment failure makes selection of rescue therapy challenging due to the potential for H. pylori resistance to antibiotics included in the initial treatment. Furthermore, second-line therapy usually exert compromised efficacy and decreased tolerability.14 The benefits and risks of eradication failure should be comprehensively balanced. Screening the appropriate patients for tests, and careful selection of diagnostic tests and initial therapies are pivotal for the effective management of H. pylori infection. The aim of this study was to provide a comprehensive review of the recent literature regarding the diagnosis and treatment of H. pylori infection in children and elderly patients.

Children

Indications for H. pylori eradication therapy in pediatric patients

The global H. pylori prevalence in children varies significantly, from 2.5% in Japan to 34.6% in Ethiopia.15 A recent study indicated that the H. pylori prevalence among Chinese school children aged 7–12 years was high and the overall infection rates were 24.1%.16 For the treatment of H. pylori infection in children, there is an urgent need to define which patients receive eradication treatment in H. pylori-positive children. In addition, it has been documented that distinct differences in immune responses and pharmacokinetics to medications exist between children and adults. In contrast to adults, children have a limited selection of antibiotics and low tolerance to the adverse effects of drugs. Additionally, children with H. pylori infection have a certain spontaneous clearance rate.16,17 Furthermore, the reinfection rate may be higher in children than in adults after eradication.18 These factors to some extent restrict the treatment of H. pylori in pediatrics. H. pylori-infected children have reduced Th1 and Th17 responses and increased Treg responses compared to infected adults in accordance with a higher level of FOXP3, TGF-β, and IL-10.19,20 These immune responses may partly contribute to the differences in gastrointestinal symptoms between H. pylori-infected children and adults. Therefore, H. pylori treatment guidelines for adults are not fully applicable to pediatric patients. Recent ESPGHAN (European Society for Pediatric Gastroenterology Hepatology and Nutrition) and NASPGHAN (North American Society for Pediatric Gastroenterology, Hepatology and Nutrition) guidelines for H. pylori infection in pediatrics recommend that the primary goal of clinical investigations of gastrointestinal symptoms should aim to determine the underlying cause of symptoms, and not solely the presence of H. pylori infection. Thus, “test and treat” strategies are not generally recommended for H. pylori infection in children.21 However, in regions with a high incidence of GC (e.g., Eastern Asia),22 this strategy may be appropriate in adolescents. In China, it is not recommended to detect H. pylori routinely in children under 14 years of age.4 Similarly, a “screening and treatment” strategy for H. pylori infection in adolescents has been supported and considered as measures to decrease the lifetime GC risk in Japan. Besides, eradication therapies for adolescents have been an effective method of controlling the next generation infection by preventing intrafamilial infection.13

In the Chinese Expert Consensus regarding the management of H. pylori infection in children (2015), recommendations for diagnostic H. pylori test include children with peptic ulcer disease, gastric MALT lymphoma, chronic gastritis, family history of GC, iron deficiency anemia (IDA) of undetermined etiology or children requiring long-term NSAIDs treatment,23 while diagnostic tests for H. pylori infection are strongly recommended in pediatric patients with peptic ulcer disease in ESPGHAN/NASPGHAN guideline.21 To identify the risk factors for giant peptic ulcers (>2.0 cm) in children from Shanghai, Tang et al24 retrospectively analyzed 19208 children who underwent gastroscopy and determined 83 patients with giant peptic ulcers. Among patients with giant peptic ulcers, 71.1% were positive for H. pylori infection, suggesting a strong association between peptic ulcer and H. pylori infection. In addition, diagnosis of H. pylori infection is recommended in patients with refractory IDA of undetermined etiology; in patients with chronic immune thrombocytopenic purpura of an unknown cause, the recommended level is low.21 However, diagnostic H. pylori tests are not recommended as part of the initial investigation in children with IDA or functional abdominal pain disorders, or causes of short stature.21 The majority of gastrointestinal symptoms in children are mainly due to functional dyspepsia, with only a small proportion classified as organic and associated with H. pylori-related diseases.25 Consistently, recent studies failed to demonstrate the differences in gastrointestinal symptoms between H. pylori-positive and H. pylori-negative children with dyspepsia in some countries,26,27 further supporting the recommendations of the ESPGHAN/NASPGHAN guidelines (2016). The association between H. pylori infection and extra-gastrointestinal symptoms in children is still controversial. A large prospective multicenter case–control study (808 subjects included) evaluating the associations between eosinophilic esophagitis and H. pylori infection failed to demonstrate the inverse association between them, in both children and adults.28,29 Hence, future studies are imperative to clarify this association.

Diagnosis of H. pylori infection in pediatrics

The ESPGHAN/NASPGHAN guideline emphasizes the importance of endoscopy in the initial diagnosis of H. pylori infection in children. Moreover, positive culture or H. pylori gastritis on histopathology with at least one other positive biopsy-based test such as the rapid urease test (RUT), molecular-based assays when available, including polymerase chain reaction (PCR), or fluorescent in situ hybridization are recommended in the initial diagnosis of H. pylori infection.21 Cultured H. pylori strains can be used for performing antibiotic susceptibility tests, which could guide the selection of anti-H. pylori treatments. In addition, the determination of initial infection should not be solely based on noninvasive tests (e.g., urea breath test (UBT), H. pylori stool antigen test, serological test). It has been reported that 13C-UBT may present false-positive results in children younger than 6 years due to the lower volume of distribution and different CO2 production rates.30 These diagnostic criteria for H. pylori infection in children are relatively strict and aim to largely avoid the excessive detection of H. pylori infection, improving the accuracy of detection. In addition, serological tests are not recommended for the diagnosis of H. pylori infection in both initial tests and confirmation of H. pylori eradication in children.21

In contrast, the guideline for determining H. pylori infection in Chinese children recommended both invasive and noninvasive tests.23 H. pylori culture with a specificity of 100% is accepted as a “gold standard” for diagnosing current H. pylori infections. However, it is difficult to use this technique as a routine diagnostic method as its sensitivity to detect H. pylori is relatively low (55%–96%).31 The accuracy of diagnostic tests rapidly decreases in populations with a low infection prevalence. Therefore, it appears reasonable that the ESPGHAN/NASPGHAN guidelines recommend the positive diagnosis of H. pylori infection be based on at least two biopsy-based tests and not solely on positive histology.21 Due to special technical requirements, H. pylori culture is challenging to perform in many regions. In Japan, a highly accurate UBT is recommended as a test for H. pylori infection in adolescents.13

Additionally, evaluation of the H. pylori status in adults or children should be performed at least 2 weeks after stopping proton pump inhibitors (PPIs) and 4 weeks after stopping antibiotic treatment. Reportedly, PPIs and antibiotics can suppress bacterial growth and replication, which may present a false-negative diagnostic result when testing for H. pylori infection.31,32 13C-UBT or monoclonal stool antigen test has been proposed to assess the H. pylori eradication, whereas invasive tests are rarely needed to verify H. pylori eradication in children with uncomplicated peptic ulcer diseases.21

Treatment of H. pylori infection in pediatrics

Treatment of H. pylori in pediatric patients remains challenging in clinical practice. Eradication failure is usually associated with several factors such as an inappropriate treatment regimen, poor compliance, and antibiotic resistance. In the latest Chinese guidelines for the management of H. pylori infection, eradication regimens against H. pylori infection are not distinguished as first-line, second-line, or third-line (except levofloxacin-containing therapy).4 The most efficient regimen should be chosen as the initial therapy. However, the selection of antibiotic classes for H. pylori eradication in children is extremely limited. Amoxicillin, clarithromycin, and metronidazole are recommended to eradicate H. pylori in pediatric patients.21,23 Currently, clarithromycin- and metronidazole-resistant H. pylori strains are prevalent worldwide,33 decreasing eradication rates in children. Zhou et al16 reported that treatment of H. pylori-infected children with standard triple therapy (PPI, amoxicillin, and clarithromycin) only achieved a 60.0% eradication rate. A prospective cohort study in Portuguese patients demonstrated a 97.8% eradication rate in H. pylori-infected children with a susceptibility-based therapy for 14 days.34 Thus, antimicrobial susceptibility-based tailored therapies are recommended for H. pylori treatment in pediatrics.21

Notably, adequate acid suppression improves the efficacy of H. pylori eradication regimens. If available, esomeprazole and rabeprazole, whose properties were less affected by CYP2C19 genetic polymorphisms, may be preferably chosen in populations with a high proportion of rapid metabolizers (e.g., Caucasians).21 Recently, a study examined the genotypes of CYP2C19 in 1083 Chinese patients and found that over 53% of patients were heterozygous extensive metabolizers (EM), 38.04% were homozygous fast type and only 8.96% were poor metabolizers.35 These results suggested that the ability to metabolize drugs varies in Chinese patients, and the determination of CYP2C19 genetic polymorphisms should receive adequate importance to achieve sufficient acid suppression in Chinese patients requiring H. pylori eradication. Potassium-competitive acid blockers (P-CABs) are a new class of gastric acid-suppressing agents. P-CABs inhibit H+/K+ ATPase-mediated gastric acid secretion in a reversible and potassium-competitive manner. Furthermore, P-CABs are thought to possess stronger inhibitory effects than PPIs. Thus, P-CABs are predicted to be more effective than PPI in H. pylori eradication therapies.36,37 Current data assessing the usefulness and safety of P-CAB in H. pylori eradication is primarily available from studies conducted in Japan. A retrospective study in Japan reviewed the medical records of 661 consecutive patients who received first-line H. pylori eradication treatment. The results suggested that 7-day P-CAB-based triple therapy (vonoprazan 20 mg + amoxicillin 750 mg + clarithromycin 200 mg twice/day) was generally well-tolerated and was more effective than a 7-day PPI-based triple therapy (lansoprazole 30 mg/rabeprazole 20 mg + amoxicillin 750 mg + clarithromycin 200 mg twice/day) in both intention-to-treat (89.1% vs. 70.9%; P < 0.001) and per-protocol analyses (91.2% vs. 71.7%; P < 0.001).37 Furthermore, another two Japanese studies evaluated the efficacy and safety of P-CAB-based triple therapy for H. pylori eradication in children and reported eradication rates of 81.3% and 85.1%. These findings indicated that a 7-day P-CAB-containing triple therapy is safe and well-tolerated in children.38,39 Vonoprazan is acid-stable and water-soluble, allowing the peak plasma concentration to be rapidly reached after administration. Compared to PPIs, vonoprazan demonstrates fast-onset, effective acid suppression, and a long-lasting effect.40 Notably, vonoprazan was also reportedly effective in clarithromycin-resistant H. pylori strains. In a clinical trial (420 patients included), the eradication rates of clarithromycin-resistant strains were 76.1% in the vonoprazan group and only 40.2% in the PPI group (amoxicillin, clarithromycin, vonoprazan or PPI).41

Sufficient antibiotic dosages and treatment durations contribute to the success of H. pylori eradication. More than a 90% eradication rate with primary treatment in per-protocol analysis should be achieved, which can avoid repeated treatments and the induction of secondary resistance by the infecting H. pylori strains.42 The ESPGHAN/NASPGHAN guideline (2016) recommends a 14-day eradication therapy containing PPI and two antibiotics in H. pylori-infected children. Metronidazole- and clarithromycin-resistant H. pylori strains are prevalent worldwide. Metronidazole resistance could be overcome with prolonged treatment duration and increased metronidazole dosage,43 while clarithromycin resistance often results in eradication failure due to challenges in therapy. Hence, the evaluation of clarithromycin resistance prior to treatment could help optimize the selection of H. pylori eradication therapies.34 In addition to culture-based susceptibility tests, PCR can be utilized to detect clarithromycin-resistant H. pylori strains.44

Dysbiosis of gut microbiota is associated with various diseases (e.g., metabolic diseases, cardiovascular diseases, cancer).45 Therefore, eradication therapy-induced disturbance of gut microbiota is also an important concern associated with H. pylori treatments. Recent studies have indicated that the gastrointestinal microbiota could be restored after H. pylori eradication in healthy teenagers and young adults.46,47 A large sample study conducted in Taiwan reported that H. pylori eradication had minimal disruption of the microbiota.48 In addition, Serrano et al49 reported that the eradication of H. pylori in infected children was associated with the restoration of the gastric microbiota to the community structure observed in the non-infected children in a cohort of 16 patients (≤13 years) presenting nausea and abdominal discomfort. However, this study was performed in a relatively small sample size, necessitating additional studies to define the impact of eradication therapy on the gut microbial composition in children. Several meta-analyses have examined the efficacy and safety of probiotic supplementation in children receiving H. pylori eradication therapy. Probiotic supplementation during eradication therapy could significantly decrease the incidence of adverse events and could increase the eradication rate during therapy.50,51 However, it is difficult to draw meaningful conclusions since studies included in these meta-analyses used different strains and concentrations of probiotics. Moreover, it has been reported that probiotic-containing yogurts could restore Bifidobacterium spp./E. coli ratio in the gut microbiota of children with H. pylori infection.52 The benefit of probiotic supplementation in H. pylori eradication therapy needs further investigation.

The elderly

Concerns regarding H. pylori treatment in elderly patients

Notably, with increased global aging, a high H. pylori prevalence has been observed among the elderly population. Concurrently, many gastrointestinal disorders (e.g., peptic ulcer disease, GC) occur much more common in the elderly.53 The eradication of H. pylori infection in elderly patients can prevent the development of H. pylori-associated diseases and significantly decrease GC incidence. However, elderly patients frequently demonstrate lower tolerance and poor compliance with anti-H. pylori eradication medication, and thus have an increased risk for drug side effects.14 Therefore, concerns regarding H. pylori treatment in the elderly need to be carefully addressed in clinical practice. Currently, there are no special consensus reports in this regard. A comprehensive benefit/risk assessment and individualization of treatments should be performed when eradicating H. pylori in the elderly population.4

In aging patients, the benefits of treating H. pylori infections remain controversial. As emphasized in the fourth and fifth H. pylori guidelines in China, H. pylori eradication before gastric mucosal atrophy and/or the absence of intestinal metaplasia can reduce the risk of GC more effectively.4,54 A prospective, randomized, placebo-controlled, population-based primary prevention study in 1630 H. pylori-infected individuals reported that H. pylori eradication could significantly decrease the GC risk in patients with precancerous lesions during a follow-up of 7.5 years after eradication treatment.55 In addition, another retrospective study in Hong Kong, China revealed that the treatment of H. pylori infection was associated with a lower GC risk, particularly in older subjects, 10 or more years after treatment.56 A blinded randomized factorial placebo-controlled trial (3365 residents included) in Linqu assessed the effects of H. pylori treatment, vitamin supplementation, and garlic supplementation in GC prevention. After a follow-up of 22 years, the inhibitory effect of H. pylori treatment on the GC risk was more significant in elderly patients compared to young or middle-aged subjects. Furthermore, H. pylori treatment could significantly reduce the GC incidence and mortality in subjects with intestinal metaplasia or dysplasia rather than in those with less severe gastric lesions.57 These results indicate that the eradication of H. pylori can be beneficial at various stages of GC. Moreover, NSAIDs and H. pylori infections are two risk factors for the development of peptic ulcers. Notably, NSAIDs are recommended as an indication for H. pylori treatment.58 Furthermore, it was reported that H. pylori infection was associated with an increased risk of neurodegeneration diseases (Alzheimer's disease, Parkinson's disease, etc.),59,60 commonly observed in an elderly population. Reportedly, H. pylori eradication in these patients was associated with improvements in symptoms associated with Alzheimer's disease61 or Parkinson's disease,62 further supporting the benefits of H. pylori treatment in the elderly.

Drug–drug interactions are also a great concern during H. pylori treatment in elderly patients.14 The potential for drug interactions should be taken into consideration when choosing a PPI to manage gastric acid-related diseases,63,64 especially in elderly patients taking multiple prescriptions.

In summary, H. pylori treatment in elderly patients should be governed by the benefit/risk assessment. H. pylori eradication can effectively prevent H. pylori-related diseases. Longer duration, higher doses of eradication therapy, selection of appropriate drugs and PPIs can contribute to higher eradication rates. In the absence of competing factors, H. pylori eradication is recommended in the elderly population.

Diagnosis and treatment of H. pylori infection in the elderly population

The recommended diagnostic H. pylori tests in the elderly are comparable to those in adults. UBT is the most popular noninvasive test to diagnose the H. pylori initial infection and is the best method to assess the efficacy of H. pylori eradication.4 RUT is recommended among patients who require gastroscopy and is not recommended for the detection of H. pylori after eradication therapy. Due to the increased GC risk with aging,53 elderly subjects may have a greater urgency for gastroscopy than younger patients. Similarly, there are no specific contraindications to the recommended eradication therapies. The selection of an eradication therapy should be based on local antibiotic-resistant patterns and the patient's history of antibiotic exposure. If the susceptibility-based test is unavailable, a validated eradication therapy with optimal efficacy should be chosen. Longer treatment durations and higher dosage are recommended to overcome antibiotic resistance, thereby increasing the efficacy of H. pylori treatment strategies. The longer duration of treatment, recommended in the American Gastroenterological Association guidelines for a general population, is also preferred among the elderly due to the high eradication rates.58 Furthermore, the lack of data indicates that increased dosage can cause more severe side effects in the elderly. If the initial therapy fails, both the determination of CYP2C19 genetic polymorphisms and susceptibility test can be used as effective methods to increase the eradication rate with a rescue therapy. Reportedly, several studies in Japan have demonstrated a superior efficacy with P-CAB in H. pylori treatment compared to PPI-contained triple therapy.36 However, further clinical trials are required to assess the efficacy and safety of P-CAB-containing regimens in different local populations.

The exploration of new therapies remains an active focus in H. pylori-related research and might further illuminate H. pylori eradication strategies. Probiotic supplementation can be considered an effective measure to reduce the incidence of adverse effects in the elderly population receiving H. pylori eradication therapy.65,66 In addition, a randomized controlled clinical trial including 232 H. pylori-infected patients (18–65 years) demonstrated that a modified dual therapy at a high dose is equally effective, safer, and less costly compared to bismuth-containing quadruple therapy.67 It is medically imperative to investigate the efficacy and safety of dual therapies in elderly patients with H. pylori infections. The role of berberine, a traditional Chinese medicine, in H. pylori treatment has also been investigated. Several lines of evidence from a meta-analysis indicated that berberine in combination with triple therapy increased H. pylori eradication rates and also reduced the overall incidence of therapy-related adverse effects.68 Additionally, a randomized, non-inferior trial conducted in China demonstrated that a berberine-quadruple regimen (PPI, amoxicillin, clarithromycin, and berberine) was comparable to the bismuth-containing quadruple regimen (PPI, amoxicillin, clarithromycin, bismuth).69 Additional studies are crucial to define the role of berberine in H. pylori eradication.

Summary

H. pylori infection is associated with various gastrointestinal diseases. Eradication of H. pylori is an effective method to prevent H. pylori-related diseases. Due to the variabilities in physiological functions and pharmacokinetics, additional evaluations and considerations in children and elderly patients should be implemented in H. pylori treatments. Gastroscopy-based tests are recommended for the diagnosis of H. pylori infection in children. According to the ESPGHAN/NASPGHAN guideline, the “test and treat” strategy for H. pylori infection is not recommended in pediatric patients. In countries with a high GC incidence, this strategy may be appropriate for adolescents. To reduce the GC risk, elderly patients should be offered H. pylori eradication therapies, unless there are competing factors. Moreover, owing to the reduced efficacy of current standard therapies, the exploration of newer regimens is urgently needed for the development of novel and efficient strategies with better eradication rates and lower adverse effects.

Funding

This study was supported by grants from the National Science and Technology Major Projects for “Major New Drugs Innovation and Development” of China(No. 2011ZX09302-007-03); Jiangxi Province Talent 555 Project; National Natural Science Foundation of China, Grant/Award Number (81670507 and 81870395); Jiangxi Provincial Department of Science and Technology (20142BAB215036, 20151BAB205041, 20171BAB205012).

Conflict of interest

None.

Edited by Yan-Gang Ren and Yi Cui

Footnotes

Peer review under responsibility of Chinese Medical Association.

Contributor Information

Quan-Ming Zou, Email: qmzou2007@163.com.

Nong-Hua Lyu, Email: lunonghua@ncu.edu.cn.

References

  • 1.Hooi J.K.Y., Lai W.Y., Ng W.K. Global prevalence of Helicobacter pylori infection: systematic review and Meta-Analysis. Gastroenterology. 2017;153:420–429. doi: 10.1053/j.gastro.2017.04.022. [DOI] [PubMed] [Google Scholar]
  • 2.International Agency for Research on Cancer WHO Schistosomes, liver flukes and Helicobacter pylori. IARC working group on the evaluation of carcinogenic risks to humans. Lyon, 7-14 june 1994. IARC Monogr Eval Carcinog Risks Hum. 1994;61:1–241. [PMC free article] [PubMed] [Google Scholar]
  • 3.Plummer M., Franceschi S., Vignat J., Forman D., de Martel C. Global burden of gastric cancer attributable to Helicobacter pylori. Int J Cancer. 2015;136:487–490. doi: 10.1002/ijc.28999. [DOI] [PubMed] [Google Scholar]
  • 4.Liu W.Z., Xie Y., Lu H. Fifth Chinese national consensus report on the management of Helicobacter pylori infection. Helicobacter. 2018;23 doi: 10.1111/hel.12475. [DOI] [PubMed] [Google Scholar]
  • 5.Sugano K., Tack J., Kuipers E.J. Kyoto global consensus report on Helicobacter pylori gastritis. Gut. 2015;64:1353–1367. doi: 10.1136/gutjnl-2015-309252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Malfertheiner P., Megraud F., O'Morain C.A. Management of Helicobacter pylori infection—the maastricht V/florence consensus report. Gut. 2016;66:6–30. doi: 10.1136/gutjnl-2016-312288. [DOI] [PubMed] [Google Scholar]
  • 7.Abadi A.T., Kusters J.G. Management of Helicobacter pylori infections. BMC Gastroenterol. 2016;16:94. doi: 10.1186/s12876-016-0496-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Miyamoto R., Okuda M., Lin Y., Murotani K., Okumura A., Kikuchi S. Rapidly decreasing prevalence of Helicobacter pylori among Japanese children and adolescents. J Infect Chemother. 2019;25:526–530. doi: 10.1016/j.jiac.2019.02.016. [DOI] [PubMed] [Google Scholar]
  • 9.Nagy P., Johansson S., Molloy-Bland M. Systematic review of time trends in the prevalence of Helicobacter pylori infection in China and the USA. Gut Pathog. 2016;8 doi: 10.1186/s13099-016-0091-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Wang B., Lv Z.F., Wang Y.H. Standard triple therapy for Helicobacter pylori infection in China: a meta-analysis. World J Gastroenterol. 2014;20:14973–14985. doi: 10.3748/wjg.v20.i40.14973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Sipponen P., Maaroos H.I. Chronic gastritis. Scand J Gastroenterol. 2015;50:657–667. doi: 10.3109/00365521.2015.1019918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Cai Q., Zhu C., Yuan Y. Development and validation of a prediction rule for estimating gastric cancer risk in the Chinese high-risk population: a nationwide multicentre study. Gut. 2019;68:1576–1587. doi: 10.1136/gutjnl-2018-317556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Kato M., Ota H., Okuda M. Guidelines for the management of Helicobacter pylori infection in Japan: 2016 revised edition. Helicobacter. 2019;24 doi: 10.1111/hel.12597. [DOI] [PubMed] [Google Scholar]
  • 14.Nguyen C.T., Davis K.A., Nisly S., Li J. Treatment of Helicobacter pylori in special patient populations. Pharmacotherapy. 2019;39:1012–1022. doi: 10.1002/phar.2318. [DOI] [PubMed] [Google Scholar]
  • 15.Mišak Z., Hojsak I., Homan M. Review: Helicobacter pylori in pediatrics. Helicobacter. 2019;24 doi: 10.1111/hel.12639. [DOI] [PubMed] [Google Scholar]
  • 16.Zhou Y., Ye Z., Huang J., Huang Y., Yan W., Zhang Y. High prevalence and low spontaneous eradication rate of Helicobacter pylori infection among schoolchildren aged 7-12 years. Acta Paediatr. 2018;107:1624–1628. doi: 10.1111/apa.14387. [DOI] [PubMed] [Google Scholar]
  • 17.Malaty H.M., Graham D.Y., Wattigney W.A., Srinivasan S.R., Osato M., Berenson G.S. Natural history of Helicobacter pylori infection in childhood: 12-Year follow-up cohort study in a biracial community. Clin Infect Dis. 1999;28:279–282. doi: 10.1086/515105. [DOI] [PubMed] [Google Scholar]
  • 18.Sivapalasingam S., Rajasingham A., Macy J.T. Recurrence of Helicobacter pylori infection in Bolivian children and adults after a population-based "screen and treat" strategy. Helicobacter. 2014;19:343–348. doi: 10.1111/hel.12137. [DOI] [PubMed] [Google Scholar]
  • 19.Razavi A., Bagheri N., Azadegan-Dehkordi F. Comparative immune response in children and adults with H. pylori infection. J Immunol Res. 2015;2015:1–6. doi: 10.1155/2015/315957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Brawner K.M., Kumar R., Serrano C.A. Helicobacter pylori infection is associated with an altered gastric microbiota in children. Mucosal Immunol. 2017;10:1169–1177. doi: 10.1038/mi.2016.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Jones N.L., Koletzko S., Goodman K. Joint ESPGHAN/NASPGHAN guidelines for the management of Helicobacter pylori in children and adolescents (update 2016) J Pediatr Gastroenterol Nutr. 2017;64:991–1003. doi: 10.1097/MPG.0000000000001594. [DOI] [PubMed] [Google Scholar]
  • 22.Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre L.A., Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA A Cancer J Clin. 2018;68:394–424. doi: 10.3322/caac.21492. [DOI] [PubMed] [Google Scholar]
  • 23.The Subspecialty Group of Gastroenterology, Society of Pediatrics, Chinese Medical Association Expert consensus on diagnosis and treatment of Helicobacter pylori infection in pediatrics. Chin J Pediatr. 2015;53:496–498. [Google Scholar]
  • 24.Tang Z., Shi J., Ji M., Shi P., Huang Z., Huang Y. The characteristics of 83 giant peptic ulcers in Chinese children: evaluation and follow-up. Saudi J Gastroenterol. 2018;24:360. doi: 10.4103/sjg.SJG_147_18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Correa S.R., Machado N.C., Carvalho M.A., Rodrigues M.A. Helicobacter pylori infection is high in paediatric nonulcer dyspepsia but not associated with specific gastrointestinal symptoms. Acta Paediatr. 2016;105:e228–e231. doi: 10.1111/apa.13347. [DOI] [PubMed] [Google Scholar]
  • 26.Ünlüsoy Aksu A., Yılmaz G., Eğritaş Gürkan Ö., Sarı S., Dalgıç B. The effect of Helicobacter pylori eradication on functional dyspepsia in Turkish children. Helicobacter. 2018;23 doi: 10.1111/hel.12497. [DOI] [PubMed] [Google Scholar]
  • 27.Szaflarska-Popławska A., Soroczyńska-Wrzyszcz A. Prevalence of Helicobacter pylori infection among junior high school students in Grudziadz, Poland. Helicobacter. 2019;24 doi: 10.1111/hel.12552. [DOI] [PubMed] [Google Scholar]
  • 28.Shah S.C., Tepler A., Peek R.M., Colombel J., Hirano I., Narula N. Association between Helicobacter pylori exposure and decreased odds of eosinophilic esophagitis—a systematic review and meta-analysis. Clin Gastroenterol Hepatol. 2019;17:2185–2198. doi: 10.1016/j.cgh.2019.01.013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Molina-Infante J., Gutierrez-Junquera C., Savarino E. Helicobacter pylori infection does not protect against eosinophilic esophagitis: results from a large multicenter case-control study. Am J Gastroenterol. 2018;113:972–979. doi: 10.1038/s41395-018-0035-6. [DOI] [PubMed] [Google Scholar]
  • 30.Leal Y.A., Flores L.L., Fuentes Pananá E.M., Cedillo Rivera R., Torres J. 13C-urea breath test for the diagnosis of Helicobacter pylori infection in children: a systematic review and meta-analysis. Helicobacter. 2011;16:327–337. doi: 10.1111/j.1523-5378.2011.00863.x. [DOI] [PubMed] [Google Scholar]
  • 31.Guarner J., Kalach N., Elitsur Y., Koletzko S. Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009. Eur J Pediatr. 2010;169:15–25. doi: 10.1007/s00431-009-1033-x. [DOI] [PubMed] [Google Scholar]
  • 32.Calvet X. Diagnosis of Helicobacter pylori infection in the proton pump inhibitor era. Gastroenterol Clin N Am. 2015;44:507–518. doi: 10.1016/j.gtc.2015.05.001. [DOI] [PubMed] [Google Scholar]
  • 33.Savoldi A., Carrara E., Graham D.Y., Conti M., Tacconelli E. Prevalence of antibiotic resistance in Helicobacter pylori: a systematic review and meta-analysis in world health organization regions. Gastroenterology. 2018;155:1372–1382. doi: 10.1053/j.gastro.2018.07.007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Silva G.M., Silva H.M., Nascimento J., Gonçalves J.P., Pereira F., Lima R. Helicobacter pylori antimicrobial resistance in a pediatric population. Helicobacter. 2018;23 doi: 10.1111/hel.12528. [DOI] [PubMed] [Google Scholar]
  • 35.Song Y., Dou F., Zhou Z. Microarray-based detection and clinical evaluation for Helicobacter pylori resistance to clarithromycin or levofloxacin and the genotype of CYP2C19 in 1083 patients. BioMed Res Int. 2018;2018:1–12. doi: 10.1155/2018/2684836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.O'Connor A., Liou J.M., Gisbert J.P., O'Morain C. Review: treatment of Helicobacter pylori infection 2019. Helicobacter. 2019;24 doi: 10.1111/hel.12640. [DOI] [PubMed] [Google Scholar]
  • 37.Suzuki S., Gotoda T., Kusano C., Iwatsuka K., Moriyama M. The efficacy and tolerability of a triple therapy containing a Potassium-Competitive acid blocker compared with a 7-Day PPI-Based Low-Dose clarithromycin triple therapy. Am J Gastroenterol. 2016;111:949–956. doi: 10.1038/ajg.2016.182. [DOI] [PubMed] [Google Scholar]
  • 38.Kakiuchi T., Matsuo M., Endo H. A Helicobacter pylori screening and treatment program to eliminate gastric cancer among junior high school students in Saga Prefecture: a preliminary report. J Gastroenterol. 2019;54:699–707. doi: 10.1007/s00535-019-01559-9. [DOI] [PubMed] [Google Scholar]
  • 39.Kusano C., Gotoda T., Suzuki S., Ikehara H., Moriyama M. Safety of first-line triple therapy with a potassium-competitive acid blocker for Helicobacter pylori eradication in children. J Gastroenterol. 2018;53:718–724. doi: 10.1007/s00535-017-1406-2. [DOI] [PubMed] [Google Scholar]
  • 40.Yang X., Li Y., Sun Y. Vonoprazan: a novel and potent alternative in the treatment of Acid-Related diseases. Dig Dis Sci. 2018;63:302–311. doi: 10.1007/s10620-017-4866-6. [DOI] [PubMed] [Google Scholar]
  • 41.Matsumoto H., Shiotani A., Katsumata R. Helicobacter pylori eradication with proton pump inhibitors or potassium-competitive acid blockers: the effect of clarithromycin resistance. Dig Dis Sci. 2016;61:3215–3220. doi: 10.1007/s10620-016-4305-0. [DOI] [PubMed] [Google Scholar]
  • 42.Graham D.Y., Lee Y.C., Wu M.S. Rational Helicobacter pylori therapy: evidence-based medicine rather than medicine-based evidence. Clin Gastroenterol Hepatol. 2014;12:177–186. doi: 10.1016/j.cgh.2013.05.028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Zhang W., Chen Q., Liang X. Bismuth, lansoprazole, amoxicillin and metronidazole or clarithromycin as first-line Helicobacter pylori therapy. Gut. 2015;64:1715–1720. doi: 10.1136/gutjnl-2015-309900. [DOI] [PubMed] [Google Scholar]
  • 44.Lascols C., Lamarque D., Costa J.M. Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H. pylori isolates from gastric biopsy specimens by real-time PCR. J Clin Microbiol. 2003;41:4573–4577. doi: 10.1128/JCM.41.10.4573-4577.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Lynch S.V., Pedersen O. The human intestinal microbiome in health and disease. N Engl J Med. 2016;375:2369–2379. doi: 10.1056/NEJMra1600266. [DOI] [PubMed] [Google Scholar]
  • 46.Gotoda T., Takano C., Kusano C. Gut microbiome can be restored without adverse events after Helicobacter pylori eradication therapy in teenagers. Helicobacter. 2018;23 doi: 10.1111/hel.12541. [DOI] [PubMed] [Google Scholar]
  • 47.He C., Peng C., Wang H. The eradication of Helicobacter pylori restores rather than disturbs the gastrointestinal microbiota in asymptomatic young adults. Helicobacter. 2019;24 doi: 10.1111/hel.12590. [DOI] [PubMed] [Google Scholar]
  • 48.Liou J.M., Chen C.C., Chang C.M. Long-term changes of gut microbiota, antibiotic resistance, and metabolic parameters after Helicobacter pylori eradication: a multicentre, open-label, randomised trial. Lancet Infect Dis. 2019;19:1109–1120. doi: 10.1016/S1473-3099(19)30272-5. [DOI] [PubMed] [Google Scholar]
  • 49.Serrano C.A., Pierre R., Van Der Pol W.J., Morrow C.D., Smith P.D., Harris P.R. Eradication of Helicobacter pylori in children restores the structure of the gastric bacterial community to that of non-infected children. Gastroenterology. 2019;157:1673–1675. doi: 10.1053/j.gastro.2019.08.017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 50.Li S., Huang X.L., Sui J.Z. Meta-analysis of randomized controlled trials on the efficacy of probiotics in Helicobacter pylori eradication therapy in children. Eur J Pediatr. 2014;173:153–161. doi: 10.1007/s00431-013-2220-3. [DOI] [PubMed] [Google Scholar]
  • 51.Feng J., Wang F., Qiu X. Efficacy and safety of probiotic-supplemented triple therapy for eradication of Helicobacter pylori in children: a systematic review and network meta-analysis. Eur J Clin Pharmacol. 2017;73:1199–1208. doi: 10.1007/s00228-017-2291-6. [DOI] [PubMed] [Google Scholar]
  • 52.Yang Y., Sheu B. Probiotics-Containing yogurts suppress Helicobacter pylori load and modify immune response and intestinal microbiota in the Helicobacter pylori-Infected children. Helicobacter. 2012;17:297–304. doi: 10.1111/j.1523-5378.2012.00941.x. [DOI] [PubMed] [Google Scholar]
  • 53.Pilotto A. Helicobacter pylori infection in older people. World J Gastroenterol. 2014;20:6364. doi: 10.3748/wjg.v20.i21.6364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 54.Liu W.Z., Xie Y., Cheng H. Fourth Chinese national consensus report on the management of Helicobacter pylori infection. J Dig Dis. 2013;14:211–221. doi: 10.1111/1751-2980.12034. [DOI] [PubMed] [Google Scholar]
  • 55.Wong B.C., Lam S.K., Wong W.M. Helicobacter pylori eradication to prevent gastric cancer in a high-risk region of China: a randomized controlled trial. J Am Med Assoc. 2004;291:187–194. doi: 10.1001/jama.291.2.187. [DOI] [PubMed] [Google Scholar]
  • 56.Leung W.K., Wong I.O.L., Cheung K.S. Effects of Helicobacter pylori treatment on incidence of gastric cancer in older individuals. Gastroenterology. 2018;155:67–75. doi: 10.1053/j.gastro.2018.03.028. [DOI] [PubMed] [Google Scholar]
  • 57.Li W., Zhang J., Ma J. Effects of Helicobacter pylori treatment and vitamin and garlic supplementation on gastric cancer incidence and mortality: follow-up of a randomized intervention trial. BMJ. 2019;366:l5016. doi: 10.1136/bmj.l5016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Chey W.D., Leontiadis G.I., Howden C.W., Moss S.F. ACG clinical guideline: treatment of Helicobacter pylori infection. Am J Gastroenterol. 2017;112:212–239. doi: 10.1038/ajg.2016.563. [DOI] [PubMed] [Google Scholar]
  • 59.Doulberis M., Kotronis G., Thomann R. Review: impact of Helicobacter pylori on Alzheimer's disease: what do we know so far? Helicobacter. 2018;23 doi: 10.1111/hel.12454. [DOI] [PubMed] [Google Scholar]
  • 60.Huang H.K., Wang J.H., Lei W.Y., Chen C.L., Chang C.Y., Liou L.S. Helicobacter pylori infection is associated with an increased risk of Parkinson's disease: a population-based retrospective cohort study. Park Relat Disord. 2018;47:26–31. doi: 10.1016/j.parkreldis.2017.11.331. [DOI] [PubMed] [Google Scholar]
  • 61.Kountouras J., Boziki M., Gavalas E. Eradication of Helicobacter pylori may be beneficial in the management of Alzheimer's disease. J Neurol. 2009;256:758–767. doi: 10.1007/s00415-009-5011-z. [DOI] [PubMed] [Google Scholar]
  • 62.Hashim H., Azmin S., Razlan H. Eradication of Helicobacter pylori infection improves levodopa action, clinical symptoms and quality of life in patients with Parkinson's disease. PLoS One. 2014;9 doi: 10.1371/journal.pone.0112330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 63.Malfertheiner P., Kandulski A., Venerito M. Proton-pump inhibitors: understanding the complications and risks. Nat Rev Gastroenterol Hepatol. 2017;14:697–710. doi: 10.1038/nrgastro.2017.117. [DOI] [PubMed] [Google Scholar]
  • 64.Wedemeyer R., Blume H. Pharmacokinetic drug interaction profiles of proton pump inhibitors: an update. Drug Saf. 2014;37:201–211. doi: 10.1007/s40264-014-0144-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 65.Zhang M., Qian W., Qin Y., He J., Zhou Y. Probiotics in Helicobacter pylori eradication therapy: a systematic review and meta-analysis. World J Gastroenterol. 2015;21:4345. doi: 10.3748/wjg.v21.i14.4345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 66.Lu C., Sang J., He H. Probiotic supplementation does not improve eradication rate of Helicobacter pylori infection compared to placebo based on standard therapy: a meta-analysis. Sci Rep. 2016;6:23522. doi: 10.1038/srep23522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 67.Yang J., Zhang Y., Fan L. Eradication efficacy of modified dual therapy compared with Bismuth-Containing quadruple therapy as a First-Line treatment of helicobacter pylori. Am J Gastroenterol. 2019;114:437–445. doi: 10.14309/ajg.0000000000000132. [DOI] [PubMed] [Google Scholar]
  • 68.Jiang X., Jiang C., Huang C. Berberine combined with triple therapy versus triple therapy for Helicobacter pylori eradication: a meta-analysis of randomized controlled trials. Evid Based Complement Alternat Med. 2018;2018 doi: 10.1155/2018/8716910. 8716910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 69.Zhang D., Ke L., Ni Z. Berberine containing quadruple therapy for initial Helicobacter pylori eradication: an open-label randomized phase IV trial. Medicine (Baltim) 2017;96 doi: 10.1097/MD.0000000000007697. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Chronic Diseases and Translational Medicine are provided here courtesy of Chinese Medical Association

RESOURCES