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. 2016 Feb 19;47(3):243–249. doi: 10.1097/PAT.0000000000000240

Molecular diagnosis of respiratory viruses

Lucy K Somerville 1,*, V Mala Ratnamohan 1, Dominic E Dwyer 1,2,3, Jen Kok 1,2,3
PMCID: PMC7130554  PMID: 25764205

Abstract

The increasing availability of nucleic acid amplification tests since the 1980s has revolutionised our understanding of the pathogenesis, epidemiology, clinical and laboratory aspects of known and novel viral respiratory pathogens. High-throughput, multiplex polymerase chain reaction is the most commonly used qualitative detection method, but utilisation of newer techniques such as next-generation sequencing will become more common following significant cost reductions. Rapid and readily accessible isothermal amplification platforms have also allowed molecular diagnostics to be used in a ‘point-of-care’ format. This review focuses on the current applications and limitations of molecular diagnosis for respiratory viruses.

Key words: Diagnostics, molecular, PCR, respiratory viruses

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