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. 2020 Mar 24;22(7):e13199. doi: 10.1111/cmi.13199

Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA

Agnieszka Sałamaszyńska‐Guz 1,✉, Izabela Serafińska 1, Paweł Bącal 2,3, Stephen Douthwaite 4,✉
PMCID: PMC7317525  PMID: 32134554

Abstract

Campylobacter jejuni is a bacterial pathogen that is generally acquired as a zoonotic infection from poultry and animals. Adhesion of C. jejuni to human colorectal epithelial cells is weakened after loss of its cj0588 gene. The Cj0588 protein belongs to the type I group of TlyA (TlyAI) enzymes, which 2′‐O‐methylate nucleotide C1920 in 23S rRNA. Slightly longer TlyAII versions of the methyltransferase are found in actinobacterial species including Mycobacterium tuberculosis, and methylate not only C1920 but also nucleotide C1409 in 16S rRNA. Loss of TlyA function attenuates virulence of both M. tuberculosis and C. jejuni. We show here that the traits impaired in C. jejuni null strains can be rescued by complementation not only with the original cj0588 (tlyA I ) but also with a mycobacterial tlyA II gene. There are, however, significant differences in the recombinant phenotypes. While cj0588 restores motility, biofilm formation, adhesion to and invasion of human epithelial cells and stimulation of IL‐8 production in a C. jejuni null strain, several of these properties are further enhanced by the mycobacterial tlyA II gene, in some cases to twice the original wild‐type level. These findings strongly suggest that subtle changes in rRNA modification patterns can affect protein synthesis in a manner that has serious consequences for bacterial pathogenicity.

Keywords: bacterial motility, biofilms, capreomycin resistance, epithelial cell invasion, rRNA 2′‐O‐methylation

1. INTRODUCTION

Orthologs of TlyA proteins are expressed in a diverse range of bacterial pathogens including Campylobacter jejuni and Mycobacterium spp. and have been linked to various roles in pathogenesis including bacterial colonisation (Sałamaszyńska‐Guz et al., 2008; Hyatt, ter Huurne, van der Zeijst, & Joens, 1994; Martino et al., 2001; Zhang, Dorrell, Wren, & Farthingt, 2002), influence on the immune response of the host (Rahman et al., 2015), haemolysis (Monshupanee, 2013; Wren et al., 1998) and antibiotic resistance (Maus, Plikaytis, & Shinnick, 2005).

In actinobacterial species, which include Mycobacterium tuberculosis, TlyA enzymes belong to the type II group (TlyAII) and have the function of 2′‐O‐methylating nucleotide C1409 in 16S rRNA and nucleotide C1920 in 23S rRNA (Johansen, Maus, Plikaytis, & Douthwaite, 2006). Slightly shorter versions of TlyA truncated at their N‐ and C‐termini are found in C. jejuni, Helicobacter pylori and Brachyspira hyodysenteriae, and belong to the type I group of enzymes (TlyAI) that methylate only at 23S rRNA nucleotide C1920 (Monshupanee, Johansen, Dahlberg, & Douthwaite, 2012; Sałamaszyńska‐Guz et al., 2018).

Loss of TlyA in C. jejuni cells results in a wide range of defects including decreased ribosome subunit association, impeded motility and reduced biofilm formation, which collectively reduce virulence (Sałamaszyńska‐Guz et al., 2018). In addition, sensitivity to the antibiotic capreomycin is altered. Complementation with natively folded variants of TlyA containing point mutations that abolish methyltransferase activity showed that all the physiological defects were caused by loss of rRNA methylation rather than absence of the protein itself (Sałamaszyńska‐Guz et al., 2018). These findings indicate that TlyA influences the physiology and pathogenicity of C. jejuni solely through its rRNA methylation activity.

Studies on the mycobacterial TlyAII and mutant derivatives of this enzyme showed that both the C1409 and C1920 methylations contribute to capreomycin binding (Monshupanee et al., 2012). These nucleotides are respectively located on the interface of the small and large ribosomal subunits (Yusupov et al., 2001) at the extremities of the binding site for capreomycin and the related tuberactinomycin drug, viomycin (Stanley, Blaha, Grodzicki, Strickler, & Steitz, 2010). While the connection between TlyA‐directed methylation and capreomycin/viomycin binding is immediately evident (Johansen et al., 2006), it remains less clear how the presence or absence of rRNA methylation would affect protein synthesis in a manner that alters pathogenic traits. Here, we address this question by equipping a tlyA‐null strain of C. jejuni with either an authentic copy its own tlyA I gene (cj0588) or the mycobacterial tlyA II gene. The influence of the different methylation patterns in the C. jejuni recombinants are shown to be linked to a range of parameters including cell motility, biofilm formation, adhesion to human epithelial cells, cell invasion and the ability to elicit an innate immune response in the host cell.

2. MATERIALS AND METHODS

2.1. Bacterial strains

The C. jejuni strains used in this study (Table 1) were grown under microaerobic conditions (BD GasPak EZ CO2 sachets, Becton Dickinson) at 37°C on brain–heart infusion (BHI) agar containing 5% (v/v) sheep blood, and in some cases supplemented with chloramphenicol at 20 μg/ml and/or kanamycin at 30 μg/ml.

Table 1.

C. jejuni strains used in this study

Strains Relevant characteristics Source/reference
C. jejuni 81–176 Wild type strain (WT) Korlath, Osterholm, Judy, Forfang, & Robinson, 1985

C. jejuni 81–176

Δcj0588

Cmr, cj0588 (tlyA I) deletion mutant Sałamaszyńska‐Guz et al., 2018

C. jejuni 81–176

Δcj0588::cj0588

Cmr, Kmr, cj0588 deleted, complemented with C. jejuni cj0588 Sałamaszyńska‐Guz et al., 2018

C. jejuni 81–176

Δcj0588::cj0588K188A

 Cmr, Kmr cj0588 deleted, complemented with K188A mutant cj0588 Sałamaszyńska‐Guz et al., 2018

C. jejuni 81–176

Δcj0588::Myco_tlyA II

Cmr, Kmr, cj0588 deleted, complemented with Mycobacterim smegmatis wild‐type tlyA II This study
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2.2. Complementation of C. jejuni cj0588‐null strain

The C. jejuni 81–176 null mutant was complemented by inserting the Mycobacterium smegmatis wild‐type tlyA II into the 121‐bp intergenic region between cj0652 and cj0653c (Javed et al., 2012; Wösten, Boeve, Koot, van Nuenen, & van der Zeijst, 1998) under control of the C. jejuni cj0183 gene promoter (Sałamaszyńska‐Guz, Grodzik, & Klimuszko, 2013). This created strain 81‐176Δcj0588::Myco_tlyA II (Table 1). All strain constructions were verified by polymerase chain reaction (PCR) and sequencing.

2.3. Primer extension

Primer extension analyses of the rRNAs were used to determine whether the tlyA I‐type and tlyA II gene products were expressed and retained their activity. RNA was prepared from C. jejuni as described previously by Douthwaite, Powers, Lee, and Noller (1989). 5′‐32P‐end‐labeled deoxynucleotide primers were hybridised to complementary regions of 16S rRNA nucleotides 1411–1429 (primer 5′‐GTGAAATCAACTCCCATGG) and 23S nucleotides 1924–1941 (primer 5′‐GAATTTCGCTACCTTAGG); Escherichia coli rRNA numbering is used throughout. Primers were extended with AMV reverse transcriptase (Roche), and the extension products were run on denaturing polyacrylamide/urea gels to detect sites of 2′‐O‐methylation (Johansen et al., 2006; Maden, Corbett, Heeney, Pugh, & Ajuh, 1995).

2.4. Minimal inhibitory concentration (MIC) determination

Overnight cultures of the C. jejuni strains were diluted to a turbidity of 0.5 McFarland standard, and 3 μl were plated onto BHI agar plates with two‐fold increases in the capreomycin concentration. The MIC values are the lowest concentration of antibiotic at which no growth was observed after incubation under microaerobic conditions for 48 hr at 37°C.

2.5. Motility

The motility of C. jejuni cells was assessed by adding 3 μl of culture (OD600 0.5) onto BHI with 0.25% agar. Plates were left to dry and were incubated under microaerobic conditions for 48 hr at 37°C before measuring cell migration.

2.6. Biofilm assays

Three‐dimension confocal microscope images of biofilms were produced from C. jejuni grown on glass slides (Millicell EZ, Milllipore). Strains were diluted in BHI broth to OD600 0.05 before aliquoting to the Millicell dishes and incubating at 37°C for 48 hr. Broth was removed, and biofilms were washed twice with water and dried at 55°C for 15 min before staining with acridine orange solution (1 μg/ml) for 30 min and rinsing twice with PBS. Biofilms were visualised at an excitation wavelength of 490 nm using a Leica white laser scanning confocal microscope (Leica TCS SP8‐WWL) with a 63× oil‐immersion lens. Three‐dimensional images were created from Z‐stacks images collected from top down to obtain an overall view of the biofilm volume and converted to TIFF files with depth‐coding using LAS X software (Leica Microsystems).

2.7. Field Emission Scanning Electron Microscopy

Visualisation of cell morphology was carried out using field emission scanning electron microscopy (FESEM). C. jejuni cells were grown on Columbia agar plates for 24 hr before harvesting and suspending in 5 ml BHI broth (at OD600 of 0.05) and cultivating for 48 hr at 37°C in 5% CO2 on glass cover slides. Cells were fixed for 24 hr in 0.1 M cacodylate buffer (pH 7.3) with 3% glutaraldehyde followed by washing for 60 min in cacodylate buffer without glutaraldehyde, and then four times for 30 min in fresh buffer followed by dehydration for 6 hr in 96% ethanol. Cells were air dried and coated with gold–palladium (2–4 nm thick) and analysed at nanometer image resolution by FESEM (MERLIN Carl Zeiss Germany) at 2–5 kV range accelerating voltage.

2.8. Adhesion and invasion assays

Caco‐2 epithelial cells, derived from a human colonic carcinoma, were seeded into a 24‐well tissue culture dishes and grown overnight at 37°C to a cell density of 105 cells per well in Eagle's minimum essential medium containing Earle's salts, 2 mM l‐glutamine, 10% fetal bovine serum, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate in a 5% CO2 (CO2 incubator, Thermo Scientific).

The C. jejuni strains were added into the wells at a multiplicity of infection (MOI) of one hundred bacteria to one epithelial cell and incubated for 2 hr to allow adhesion and invasion of the Caco‐2 cells. The Caco‐2 monolayers were washed three times with PBS to remove unattached bacteria. A portion of the Caco‐2 cells was then lysed with 0.1% Triton X‐100 to estimate the total complement of bacterial cells. The remaining Caco‐2 cells were incubated for a further 2 hr in modified minimal essential medium with 100 μg gentamicin ml−1 to kill extracellular bacteria, while retaining viable internalised bacteria. Bacteria adhering to and internalised by the Caco‐2 cells were tallied by serial dilution in phosphate‐buffered saline (PBS) and plating on BHI agar.

2.9. Survival assay

C. jejuni survival was quantified in RAW 264.7 macrophages cultured in RPMI medium with 10% fetal bovine serum at 37°C in 5% CO2 atmosphere (CO2 incubator, Thermo Scientific). Tissue culture trays (24‐well) were seeded with 2 × 105 macrophages per ml and incubated for 24 hr prior to inoculating with C. jejuni at an approximate MOI of 100. Infected macrophage monolayers were incubated for 2 hr before killing extracellular bacteria as described above. Surviving bacteria were monitored (as above) at 3, 6, 12, 24 and 48 hr post‐infection.

2.10. Innate immune response in epithelial cells

Production of interleukin of IL‐8 by Caco‐2 cells was taken as an indicator of the extent to which by C. jejuni strains provoked an innate immune response. Caco‐2 cells were seeded in 24‐well plates and infected with bacteria as described above. Cell supernatants were assayed after one day using a human IL‐8 ELISA kit (Merck). Optical densities were measure with a microplate reader (Epoch spectrophotometer, BioTek Instruments) and normalised relative to negative controls (no C. jejuni cells) using the instrument supplier's software.

3. RESULTS

3.1. Mycobacterial TlyAII specifically methylates two nucleotides in C. jejuni rRNAs

The wild type tlyA II gene from M. smegmatis was introduced into the C. jejuni null strain 81‐176Δcj0588. Screening the rRNAs from this recombinant by primer extension showed that expression of the mycobacterial TlyAII enzyme effectively modified nucleotide C1409 in C. jejuni 16S rRNA and C1920 in the 23S rRNA (E. coli rRNA numbering) (Figure 1).

Figure 1.

Figure 1

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In vivo activity of mycobacterial TlyAII in C. jejuni. Gel autoradiograms of primer extensions on rRNA from C. jejuni strains. Extensions on 16S and 23S rRNAs from the mutant strain C. jejuni 81–176 Δcj0588 and from the same strain complemented with mycobacterial tlyA II (C. jejuni 81–176 Δcj0588::Myco_tlyA II). Decreasing the dGTP concentrations (100, 10 and 1 μM, marked with wedges) intensifies reverse transcription termination at 16S rRNA C1409 and 23S rRNA C1920 when these nucleotides are 2′‐O‐methylated. Lanes C, U, A and G are dideoxy‐sequencing reactions on unmodified C. jejuni rRNAs. Control lanes represent primers and reaction mixture without rRNA template

These modifications resulted in a concomitant increase in the sensitivity of C. jejuni to capreomycin. The MIC values for capreomycin in strains without a tlyA gene (81176Δcj0588) or with an inactivated version of the gene (81176Δcj0588::K188A) were consistently 64 μg/ml. The capreomycin MIC was lowered to 32 μg/ml by expression of the mycobacterial tlyA II gene that was the same value seen for cells expressing the original tlyA I gene cj0588 that methylates only at 23S rRNA nucleotide C1920.

3.2. Motility and rRNA methylation

Loss or inactivation of its natural tlyA I gene results in decreased C. jejuni motility. Under the microaerobic conditions at 37°C employed here, C. jejuni null strains exhibit only one‐third of the mobility of the wild‐type (Figure 2). Complementation with an active copy of cj0588 goes some way to restoring mobility to wild‐type levels. Similarly, introduction of the mycobacterial tlyA II gene into the null strain partially rescues the cell's motility (Figure 2).

Figure 2.

Figure 2

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Motility of the C. jejuni strains. Agar plates with (a) WT‐wild type strain, (b) Δcj0588, (c) Δcj0588::cj0588, (d) Δcj0588::cj0588K188A and (e) Δcj0588::Myco_tlyA II strains of C. jejuni grown for 48 hr. The morphologies of the corresponding stains (including flagella) were visualised by Field Emission Scanning Electron Microscopy (FESEM). Strain motility is summarised in the histogram, where values represent the means ± SEM of three independent experiments measuring distances migrated over 48 hr. There was no significant difference for migration of the WT compared to Δcj0588::M.smeg_tlyA II, whereas significant differences were observed for Δcj0588 versus Δcj0588::M.smeg_tlyA II (p < .05), and for Δcj0588 versus Δcj0588::cj0588 (p < .05)

3.3. Biofilm formation is influenced by the rRNA methylation pattern

Biofilms were visualised using confocal laser microscopy producing three‐dimensional images of these structures (Figures 3 and 4). Inactivation of the cj0588 gene reduces the cell's ability to form biofilms, and this effect is rescued by introduction of a functional copy of this gene. The null strain formed a thin biofilm that failed cover the whole surface and reached a depth of only 3.9 μm in its thickest region. Complementation with an active cj0588 gene fully restored biofilm density to 5.9 μm, comparable to that of the wild‐type strain (5.2 μm). Surprisingly, transformation of the null strain with the mycobacterial tlyA II gene (forming the 81176Δcj0588::Myco_tlyA II strain) not only rescued the phenotype but supported uniform biofilm formation at a density of 7.5 μm, significantly surpassing that the wild‐type strain (Figure 4).

Figure 3.

Figure 3

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Images of biofilm structures produced by C. jejuni (a) WT‐wild type strain, (b) Δcj0588, (c) Δcj0588::cj0588, (d) Δcj0588::cj0588K188A and (e) Δcj0588::Myco_tlyA II strains visualised by confocal laser microscopy. Relative biofilm depths are color‐coded as shown

Figure 4.

Figure 4

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Analysis of the Figure 3 images showing the biofilm density produced by C. jejuni (a) WT‐wild type strain, (b) Δcj0588, (c) Δcj0588::cj0588, (d) Δcj0588::cj0588K188A and (e) Δcj0588::Myco_tlyA II strains. (f) Histogram summarising the biofilm data, color‐coded as in Figure 2. Experiments were carried out in triplicate and representative images are shown here. p < .001 for WT versus Δcj0588; p < .005 for WT versus Δcj0588::M.smeg_tlyA II

3.4. Adhesion and invasion of the C. jejuni strains on Caco‐2 cells

After inactivation of TlyA‐directed methylation, the capacity of C. jejuni to adhere to the surface and to invade Caco‐2 human colon epithelial cells was reduced to less than half that of the wild‐type (Figure 5). Both adhesion and internalisation were restored (to 93 and 103% wild‐type levels, respectively) by complementation of the null strain with cj0588. This effect was more marked after complementation with the mycobacterial gene where the C. jejuni tlyA II recombinants became about one‐third more adept than the wild‐type at sticking to Caco‐2 cells enabling the pathogen to invade the epithelial cells 25% more effectively (Figure 5). The bacterial strains' ability to enter the Caco‐2 cells was roughly proportional to their adhesive properties and thus the invasive index, which is the proportion of the surface‐adhered bacteria that actually enter the eukaryotic cell, was fairly constant (i.e., varied less than 25%) for the different C. jejuni recombinants.

Figure 5.

Figure 5

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(a) Adhesion onto, and (b) invasion into Caco‐2 cells by C. jejuni strains. Values represent means ± SEM of three independent experiments. Adhesion p < .05 for WT versus Δcj0588 and Δcj0588::Myco_tlyA II; invasion p < .005 for WT versus Δcj0588 and Δcj0588::Myco_tlyA II

Subsequent to invasion, the virulence of the C. jejuni attack was inferred from the IL‐8 response within the Caco‐2 epithelial cell line. Consistent with the adhesion/invasion data, the mildest reaction was seen with the cj0588‐deletion strain and the inactive K188A variant, where IL‐8 levels were barely above background (Figure 6). The wild‐type and complemented strains with an active cj0588 gene produced a clearer response, approximately doubling the amount of IL‐8. The highest level of IL‐8 was observed with C. jejuni expressing the mycobacterial tlyA II gene, reflecting the augmented adhesion/invasion properties of this strain in the Caco‐2 cell model.

Figure 6.

Figure 6

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IL‐8 secretion by Caco‐2 induced by C. jejuni strains. The negative control shows the background level of IL‐8 secretion measured in the absence of C. jejuni cells. Values represent means ± SEM of three independent experiments. p < .05 for WT versus Δcj0588; p < .01 for WT versus Δcj0588::Myco_tlyA II

3.5. Influence of the rRNA methylation pattern on bacterial survival in macrophages

Another important aspect of C. jejuni virulence is its ability to survive within host cells, and this was tested here using a RAW264.7 macrophage model system. While C. jejuni strains expressing functional TlyA proteins attached to and invaded the macrophages significantly more avidly than null strains, their survival within macrophages was not improved (Table 2), and all the C. jejuni strains within macrophages were killed by 48 hr.

Table 2.

Survival of C. jejuni strains within macrophages

Hours after infection Colony forming units (cfu) of C. jejuni strains surviving
WT Δcj0588 Δcj0588::cj0588 Δcj0588::cj0588 K188A Δcj0588::Myco tlyA II
3 281,035 ± 29,856 45,886 ± 30,052 274,700 ± 69,296 23,669 ± 10,394 284,701 ± 69,296
6 142,570 ± 3,110 35,375 ± 7,443 155,000 ± 34,156 14,525 ± 2,227 92,600 ± 10,465
12 3,002 ± 87 1,268 ± 381 3,446 ± 702 481 ± 99 2,713 ± 636
24 300 ± 36 125 ± 77 200 ± 13 35 ± 23 36 ± 23
48 0 0 0 0 0
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Note: Macrophage RAW264.7 cell line samples were each infected with 107 cfu of the various C. jejuni strains (time zero). Viable intracellular C. jejuni cells are tabulated with shading to indicate >200,000; 50,000 to 200,000; 2,000 to 50,000; 200 to 2,000; and 20 to 200 surviving cells over 24 hr. No viable C. jejuni cells were detected at 48 hr. The cfu values are means ± SEM of three independent experiments; p < .05 for WT versus Δcj0588; p < .01 for WT versus Δcj0588::Myco_tlyA II.

4. DISCUSSION

The natural version of the TlyA methyltransferase found in C. jejuni is a type I (TlyAI) enzyme encoded by the cj0588 gene, and stoichiometrically methylates the 2´‐O‐ribose of 23S rRNA nucleotide C1920 (Sałamaszyńska‐Guz et al., 2018). Type II (TlyAII) variants, found in some Gram‐positive bacteria including the Actinobacteria, methylate not only at nucleotide C1920 but also at 16S rRNA nucleotide C1409 (Johansen et al., 2006; Monshupanee et al., 2012). Both these nucleotides are effectively modified in the natural M. smegmatis host (Monshupanee et al., 2012), and the mycobacterial tlyA II gene product retains the same specificity when transferred to and expressed from the C. jejuni chromosome (Figure 1).

We have previously shown that a possession of an active TlyAI (Cj0588) methyltransferase is a prerequisite for C. jejuni to function effectively as a pathogen (Sałamaszyńska‐Guz & Klimuszko, 2008; Sałamaszyńska‐Guz et al., 2014). The role of TlyAI in pathogenicity is linked to a series of factors including ribosomal subunit interaction, cell motility and biofilm formation, all of which were depressed in cj0588 null strains (Sałamaszyńska‐Guz et al., 2018). These properties are rescued by complementation with an active copy of cj0588, although no rescue occurs after eliminating the catalytic activity of the enzyme by introducing point mutations into cj0588. These methyltransferase mutants retained their tertiary structure and their substrate/cofactor binding affinities, and thus the reduction in C. jejuni virulence was solely a consequence of the mutant enzymes' inability to methylate the rRNA (Sałamaszyńska‐Guz et al., 2018).

Homologs of this enzyme have also been linked to virulence in other bacterial pathogens. Mutation of the tlyA I homolog in B. hyodysenteriae reduces virulence (Hyatt et al., 1994), while loss of the enzyme's function in H. pylori lowers adhesion to human gastric adenocarcinoma (AGS) cells and prevents colonisation of the gastric mucosa (Martino et al., 2001; Zhang et al., 2002). The TlyAII variant of this enzyme promotes survival of M. tuberculosis in macrophages (Rahman et al., 2015) and aids the binding of capreomycin to ribosomes (Maus et al., 2005). Several other endogenous rRNA methylations (reviewed in Purta, O'Connor, Bujnicki, & Douthwaite, 2009) have also been noted to promote ribosome–antibiotic interactions within bacterial pathogens (LaMarre, Howden, & Mankin, 2011; Sergeeva, Bogdanov, & Sergiev, 2015). In the present study, we demonstrate that the defective virulence traits exhibited in C. jejuni tlyA null strains can be rescued by a mycobacterial tlyA II ortholog, and that some phenotypic traits of the recombinant strain are distinctly different from the original wild‐type and recombinants rescued with the original cj0588 (tlyA I ) gene.

The lower mobility of the C. jejuni null strain was restored to roughly the same extent by the wild‐type C. jejuni tlyA I gene and the mycobacterial tlyA II gene (Figure 2). However, the recombinants differed in their capacity to form biofilms. Complementation with tlyA I re‐establishes wild‐type levels, while cells transformed with tlyA II form significantly denser biofilms (Figures 3 and 4). Visualisation of the cells using FESEM revealed that the cell morphology and flagella structure of the tlyA I (Sałamaszyńska‐Guz et al., 2018) and the tlyA II recombinants (Figure 2) were indistinguishable from that of wild‐type C. jejuni cells. Surprisingly, therefore, loss of TlyA function does not affect flagella morphology despite the central role of flagella in C. jejuni pathogenesis (Guerry, 2007; Svensson, Pryjma, & Gaynor, 2014).

The tlyA II homolog of M. tuberculosis, which is 84% similar in amino acid sequence and functionally identical to the M. smegatis tlyA II (Monshupanee et al., 2012), plays an important role in the survival of the bacillus during infection (Rahman et al., 2015). M. tuberculosis cells lacking tlyA II become more susceptible to autophagy, and animals infected with this mutant strain exhibit increased immune response, reduced bacillary load and improved survival rates than when infected with wild‐type bacilli (Rahman et al., 2015). Our findings here suggest that the mycobacterial tlyA II gene supports a comparable set of virulence traits in C. jejuni.

The ability of C. jejuni to attach to and invade human epithelial cells is central to its pathogenicity and was notably impaired by loss of TlyA‐directed methylation (Figure 5). Restoring TlyAI methylation by complementation of C. jejuni with cj0588 rescued its adhesion to and invasion of Caco‐2 cells. Surprisingly, these features were not only rescued by the mycobacterial tlyA II but this recombinant clung to and entered the epithelial cells significantly more effectively than the original wild‐type strain C. jejuni (Figure 5).

When under attack by pathogenic bacteria, epithelial cells secrete chemotactic mediators (Eckmann, Kagnoff, & Fierer, 1993) and consistent with this, C. jejuni induces human‐derived epithelial cell lines to release pro‐inflammatory chemokines including the interleukin, IL‐8 (Hickey, Baqar, Bourgeois, Ewing, & Guerry, 1999; Watson & Galan, 2005). In related studies of Campylobacter invasion, cytolethal distending toxin, outer membrane vesicles and the flagella activate the host cell's toll‐like receptors to elicit secretion of IL‐8 (Zheng, Meng, Zhao, Singh, & Song, 2008). The absence of tlyA activity is shown here to reduce the ability of C. jejuni to trigger the IL‐8 innate immune response in Caco‐2 cells (Figure 6). Induction of IL‐8 was restored by complementing null strains with an active tlyA gene, where the tlyA II gene produced the most marked stimulation increasing IL‐8 production to twice that with wild‐type C. jejuni.

These observations raise a number of questions. First, why a single ribose methylation (at 23S rRNA nucleotide C1920) would be a prerequisite for successful infection by C. jejuni and how an additional methylation (at 16S rRNA nucleotide C1409) would further improve its capacity to infect. The two TlyAII methylations are located approximately 20 Å apart on opposite sides of the ribosomal subunit interface and lie adjacent to the capreomycin binding site (Johansen et al., 2006). Both methylations have been shown to contribute individually to drug binding (Monshupanee et al., 2012). From the crystal structure of capreomycin‐bound ribosomes (Stanley et al., 2010), the methylations are slightly too far apart to make contact with the drug. However, they are nevertheless positioned where they might lubricate the relative rotational movement of the subunits during translation (Yusupov et al., 2001), a process where one of the pivoted subunit conformations is favoured for drug binding (Ermolenko et al., 2007). Each of the methylations thus contributes to ribosome function, and our working hypothesis (presently being tested) is that changes in the methylation pattern subtly skew the relative synthesis rates of different proteins in the bacterium, with this being ultimately reflected in altered virulence properties.

Another, and potentially more important, question is whether it would make a difference in the real world if C. jejuni were to attain both methylations through changes in its own tlyA gene or via transfer of a tlyA II ortholog from another bacterium. The ability of C. jejuni to adhere to epithelial cells is dependent on having a functional tlyA gene and is enhanced with a tlyA II‐type gene, and these adhesive properties determine the degree of cell invasion (Figure 5). An additional aspect to be taken into consideration is that C. jejuni pathogenicity depends on its ability to survive subsequent to phagocytosis. On the one hand, the RAW 264.7 macrophage data (Table 2) show that significantly fewer C. jejuni survive when they lack an active cj0588 gene and that complementing the cells with an active copy of cj0588 or the mycobacterial tlyA II gene restores their initial survival rates. This observation is consistent with the role of tlyA II mentioned above, where it supports the survival within macrophages of its authentic host, M. tuberculosis (Rahman et al., 2015). However, when extending the time frame of observations past the initial phagocytotic event, we find that the C. jejuni‐tlyA II cells are no more resilient after 12 hours (and in fact appear slightly more frail) than strains expressing cj0588 (Table 2). In this case, the wild‐type cj0588 gene affords better protection against the host's defences, and it thus remains an open question to what extent the superior adhesion properties conferred by tlyA II represent a route towards increased C. jejuni virulence.

CONFLICT OF INTEREST

The authors have no conflicts of interest to declare.

ACKNOWLEDGEMENTS

Support from the National Science Centre [2018/30/M/NZ6/00429], KNOW (the Leading National Research Centre) Scientific Consortium “Healthy Animal–Safe Food”, decision of Polish Ministry of Science and Higher Education, no. 05‐1/KNOW2/2015 to AS‐G, and from the Danish Research Agency (FNU‐rammebevilling 10–084554) to SD is gratefully acknowledged. The authors thank Prof. Zdzisław Gajewski Warsaw University of Live Sciences – SGGW, Warsaw, Poland, for providing access to the Leica TCS SP8‐WWL confocal microscope.

Sałamaszyńska‐Guz A, Serafińska I, Bącal P, Douthwaite S. Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA. Cellular Microbiology. 2020;22:e13199 10.1111/cmi.13199

Funding information Krajowy Naukowy Ośrodek Wiodący, Grant/Award Number: 05‐1/KNOW2/2015; Narodowe Centrum Nauki, Grant/Award Number: 2018/30/M/NZ6/00429; Natur og Univers, Det Frie Forskningsråd, Grant/Award Number: 10‐084554

Contributor Information

Agnieszka Sałamaszyńska‐Guz, Email: agnieszka_salamaszynska_guz@sggw.pl.

Stephen Douthwaite, Email: srd@bmb.sdu.dk.

REFERENCES

  1. Douthwaite, S. , Powers, T. , Lee, J. Y. , & Noller, H. F. (1989). Defining the structural requirements for a helix in 23S ribosomal RNA that confers erythromycin resistance. Journal of Molecular Biology, 209(4), 655–665. [DOI] [PubMed] [Google Scholar]
  2. Eckmann, L. , Kagnoff, M. F. , & Fierer, J. (1993). Epithelial cells secrete the chemokine interleukin‐8 in response to bacterial entry. Infection and Immunity, 61(11), 4569–4574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ermolenko, D. N. , Spiegel, P. C. , Majumdar, Z. K. , Hickerson, R. P. , Clegg, R. M. , & Noller, H. F. (2007). The antibiotic viomycin traps the ribosome in an intermediate state of translocation. Nature Structural & Molecular Biology, 14(6), 493–497. [DOI] [PubMed] [Google Scholar]
  4. Guerry, P. (2007). Campylobacter flagella: Not just for motility. Trends in Microbiology, 15, 456–461. [DOI] [PubMed] [Google Scholar]
  5. Hickey, T. E. , Baqar, S. , Bourgeois, L. , Ewing, C. P. , & Guerry, P. (1999). Campylobacter jejuni stimulated secretion of interleukin‐8 by INT407 cells. Infection and Immunity, 67(1), 88–93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hyatt, D. R. , ter Huurne, A. A. , van der Zeijst, B. A. , & Joens, L. A. (1994). Reduced virulence of Serpulina hyodysenteriae hemolysin‐negative mutants in pigs and their potential to protect pigs against challenge with a virulent strain. Infection and Immunity, 62(6), 2244–2248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Javed, M. A. , Cawthraw, S. A. , Baig, A. , Li, J. , McNally, A. , Oldfield, N. J. , … Manning, G. (2012). Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni . Infection and Immunity, 80(7), 2361–2370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johansen, S. K. , Maus, C. E. , Plikaytis, B. B. , & Douthwaite, S. (2006). Capreomycin binds across the ribosomal subunit interface using tlyA‐encoded 2′‐O‐methylations in 16S and 23S rRNAs. Molecular Cell, 23(2), 173–182. [DOI] [PubMed] [Google Scholar]
  9. Korlath, J. A. , Osterholm, M. T. , Judy, L. A. , Forfang, J. C. , & Robinson, R. A. (1985). A point‐source outbreak of campylobacteriosis associated with consumption of raw milk. Journal of Infectious Diseases, 152(3), 592–596. [DOI] [PubMed] [Google Scholar]
  10. LaMarre, J. M. , Howden, B. P. , & Mankin, A. S. (2011). Inactivation of the indigenous methyltransferase RlmN in Staphylococcus aureus increases linezolid resistance. Antimicrob Agents and Chemotherapy, 55(6), 2989–2991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maden, B. E. H. , Corbett, M. E. , Heeney, P. A. , Pugh, K. , & Ajuh, P. M. (1995). Classical and novel approaches to the detection and localization of the numerous modified nucleotides in eukaryotic ribosomal RNA. Biochimie, 77(1–2), 22–29. [DOI] [PubMed] [Google Scholar]
  12. Martino, M. C. , Stabler, R. A. , Zhang, Z. W. , Farthing, J. G. , Wren, B. W. , & Dorrell, N. (2001). Helicobacter pylori pore‐forming orthologue TlyA possesses in vitro hemolytic activity and has a role in colonization of gastric mucosa. Infection and Immunity, 69(3), 1697–1703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maus, C. E. , Plikaytis, B. B. , & Shinnick, T. M. (2005). Mutation of tlyA confers capreomycin resistance in Mycobacterium tuberculosis . Antimicrobial Agents and Chemotherapy, 49(2), 571–577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Monshupanee, T. (2013). Increased bacterial hemolytic activity is conferred by expression of TlyA methyltransferase but not by its 2′‐O‐methylation of the ribosome. Current Microbiology, 67, 61–68. [DOI] [PubMed] [Google Scholar]
  15. Monshupanee, T. , Johansen, S. K. , Dahlberg, A. E. , & Douthwaite, S. (2012). Capreomycin susceptibility is increased by TlyA‐directed 2′‐O‐methylation on both ribosomal subunits. Molecular Microbiology, 85(6), 1194–1203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Purta, E. , O'Connor, M. , Bujnicki, J. M. , & Douthwaite, S. (2009). YgdE is the 2′‐O‐ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA. Molecular Microbiology, 72, 1147–1158. [DOI] [PubMed] [Google Scholar]
  17. Rahman, M. A. , Sobia, P. , Dwivedi, V. P. , Bhawsar, A. , Singh, D. K. , Sharma, P. , … Das, G. (2015). Mycobacterium tuberculosis TlyA negatively regulates Th1 and Th17 differentiation and promotes tuberculosis pathogenesis. Journal of Biological Chemistry, 5(23), 14407–14417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sałamaszyńska‐Guz, A. , Grodzik, M. , & Klimuszko, D. (2013). Mutational analysis of cj0183 Campylobacter jejuni promoter. Current Microbiology, 67(6), 696–702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sałamaszyńska‐Guz, A. , & Klimuszko, D. (2008). Functional analysis of the Campylobacter jejuni cj0183 and cj0588 genes. Current Microbiology, 56(6), 592–596. 10.1007/s00284-008-9130-z [DOI] [PubMed] [Google Scholar]
  20. Sałamaszyńska‐Guz, A. , Rose, S. , Lykkebo, C. A. , Taciak, B. , Bącal, P. , Uśpieński, T. , & Douthwaite, S. (2018). Biofilm Formation and Motility Are Promoted by Cj0588‐Directed Methylation of rRNA in Campylobacter jejuni . Frontiers in Cellular and Infection Microbiology, 7, 533 10.3389/fcimb.2017.00533 [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sałamaszyńska‐Guz, A. , Taciak, B. , Kwiatek, A. , & Klimuszko, D. (2014). The Cj0588 protein is a Campylobacter jejuni RNA methyltransferase. Biochemical and Biophysical Research Communications, 448(3), 298–302. [DOI] [PubMed] [Google Scholar]
  22. Sergeeva, O. V. , Bogdanov, A. A. , & Sergiev, P. V. (2015). What do we know about ribosomal RNA methylation in Escherichia coli? Biochimie, 117, 110–118. [DOI] [PubMed] [Google Scholar]
  23. Stanley, R. E. , Blaha, G. , Grodzicki, R. L. , Strickler, M. D. , & Steitz, T. A. (2010). The structures of the anti‐tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome. Nature Structural & Molecular Biology, 17(3), 289–293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Svensson, S. L. , Pryjma, M. , & Gaynor, E. C. (2014). Flagella‐mediated adhesion and extracellular DNA release contribute to biofilm formation and stress tolerance of Campylobacter jejuni . PLoS One, 9, e106063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Watson, R. O. , & Galan, J. E. (2005). Signal transduction in Campylobacter jejuni induced cytokine production. Cellular Microbiology, 7(5), 655–665. [DOI] [PubMed] [Google Scholar]
  26. Wösten, M. M. , Boeve, M. , Koot, M. G. , van Nuenen, A. C. , & van der Zeijst, B. A. (1998). Identification of Campylobacter jejuni promoter sequences. Journal of Bacteriology, 180(3), 594–599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wren, B. W. , Stabler, R. A. , Das, S. S. , Butcher, P. D. , Mangan, J. A. , Clarke, J. D. , … Stoker, N. G. (1998). Characterization of a haemolysin from Mycobacterium tuberculosis with homology to a virulence factor of Serpulina hyodysenteriae . Microbiology, 144(Pt5), 1205–1211. [DOI] [PubMed] [Google Scholar]
  28. Yusupov, M. M. , Yusupova, G. Z. , Baucom, A. , Lieberman, K. , Earnest, T. N. , Cate, J. H. , & Noller, H. F. (2001). Crystal structure of the ribosome at 5.5 Å resolution. Science, 292(5518), 883–896. [DOI] [PubMed] [Google Scholar]
  29. Zhang, Z. W. , Dorrell, N. , Wren, B. W. , & Farthingt, M. J. (2002). Helicobacter pylori adherence to gastric epithelial cells: A role for non‐adhesin virulence genes. Journal of Medical Microbiology, 51(6), 495–502. [DOI] [PubMed] [Google Scholar]
  30. Zheng, J. , Meng, J. , Zhao, S. , Singh, R. , & Song, W. (2008). Campylobacter‐induced interleukin‐8 secretion in polarized human intestinal epithelial cells requires Campylobacter‐secreted cytolethal distending toxin‐ and Toll‐like receptor‐mediated activation of NF‐kappaB. Infection and Immunity, 76(10), 4498–4508. [DOI] [PMC free article] [PubMed] [Google Scholar]

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