Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate-induced colitis

Abstract

Clostridium butyricum (CB), a probiotic, is a gram-positive obligate anaerobic bacillus with acid and heat resistant properties. Previous studies have reported that CB has beneficial effects in intestinal diseases and regulates intestinal function. The aim of the present study was to investigate the protective effects and mechanisms of CB on the intestinal barrier function. Mice were randomly divided into three experimental groups (n=15 mice/group), including control, dextran sodium sulfate (DSS) and DSS + CB. In the DSS and DSS + CB groups colitis was induced with 3% DSS dissolved in drinking water for 7 days. DSS + CB group mice were co-treated daily with 200 µl (2x10⁸ CFU) CB solution via gavage. The intestinal mucosal barrier function in mice was assessed by measuring FITC-labeled 4-kDa dextran (molecular weight, 4,000 Da) flux and by analyzing the expression of tight junction (TJ)-related proteins using western blot analysis. In addition, the secretion levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and IL-13, and the concentration of malondialdehyde, glutathione and superoxide dismutase were detected using ELISAs to determine inflammation and oxidative stress, respectively. The activation status of the Akt/mTOR signaling pathway was also investigated using western blot analysis. The results demonstrated that, in mice with DSS-induced colitis treatment, co-treatment with CB attenuated colitis symptoms and intestinal permeability, increased the expression levels of TJ-related proteins, decreased TNF-α, IL-1β and IL-13 secretion levels but increased those of IL-10, and reduced oxidative stress. Additionally, CB elevated the phosphorylation of Akt, mTOR and p70 ribosomal protein S6 kinase. Collectively, the present results indicated that CB protected intestinal barrier function and decreased intestinal mucosal permeability via upregulating the expression levels of TJ-related proteins in a mouse model of DSS-induced colitis. Moreover, the results suggested that the effects of CB could be mediated by the Akt/mTOR signaling pathway.

Introduction

The main function of the intestinal epithelium is to absorb water and nutrients to maintain life, and the transport of these substances should be tightly regulated in order to maintain homeostasis (1). The intestinal epithelium is an important structure, responsible for transporting water and nutrients, and constitutes a physical barrier regulating the selective paracellular permeability to ions and molecules (2). Adjacent intestinal epithelial cells are tightly packed together and are connected via three types of junctional complexes, namely desmosomes, adherens junctions and tight junctions (TJs) (3). TJs are the most apical junctions along the lateral surface, and are the main structure regulating epithelial barrier function (4). TJs are composed of transmembrane proteins, which include claudins, occludin and tricellulin, and cytosolic scaffold proteins, such as zona occludens proteins (ZO)1-3, Afadin (AF-6) and cingulin (2).

Ulcerative colitis (UC) is a chronic gastrointestinal disorder characterized by diffuse mucosal inflammation of the colon. In UC, pathological lesions are mainly located in the rectum, affecting a part or the whole large intestine (5). Moreover, the clinical course of the disease is characterized by remissions and exacerbations (6). It has been reported that dysregulation of the intestinal mucosal barrier contributes to the development, perpetuation and severity of inflammatory bowel disease (IBD) (7). At the same time, IBD may also contribute to a leaky epithelial barrier due to the inflammatory microenvironment (8). In UC, impaired complexity of TJs and downregulation of TJ-related proteins have been considered as the main mechanisms mediating the dysfunction of the intestinal mucosal barrier (9,10). Commonly used drugs for UC treatment include 5-aminosalicylic acid, immunosuppressants and biological agents (5). Furthermore, given the continuous progress in the research on intestinal microorganisms, probiotics have become a novel treatment strategy for UC.

The gastrointestinal tract contains a large and complex ecology of microorganisms. For example, it is estimated that ~100 trillion microbes reside in the human adult gut, collectively known as the gut microbiota (11). The mutually beneficial relationship between the host and its gut microbiota contributes to host health, as gut microbiota are involved in several biological processes, including the absorption of nutrients, protection against infections, maturation of the immune system and regulation of metabolism (12). The International Symposium of the International Scientific Association of Probiotics and Prebiotics defined probiotics as ‘the living microorganisms that when administered in adequate amounts, confer health benefits on the host’ (13). Clostridium butyricum (CB) is a gram-positive obligate anaerobic bacillus. Compared with other non-sporulating probiotics, CB is resistant to the effects of gastric acid and temperature, thus making its investigation and use easier (14,15). It has been reported that butyrate, an important type of short chain fatty acid (SCFA) produced by CB, exhibits positive effects on the maintenance of intestinal barrier function (16,17). Therefore, CB is effectively used in clinical practice to improve the intestinal barrier function and to treat UC. However, its exact underlying mechanisms require further investigation.

In the present study, a dextran sodium sulfate (DSS)-induced colitis model was established in C57BL/6 mice to investigate the potential effects of CB on the expression levels of TJ-related proteins, intestinal inflammation and oxidative stress. It has been well established that activation of the Akt/mTOR pathway and its downstream molecules, including p70 ribosomal protein S6 kinase (S6K) and eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1), regulate protein synthesis (18). Therefore, the present study further investigated the role of CB on the regulation of TJ-related protein expression levels via the Akt/mTOR pathway.

Materials and methods

Animals and mouse model

A total of 45 C57BL/6 mice (males; age, 6 weeks; weight, 16-20 g) were obtained from the Beijing Vital River Laboratory Animal Technology Co., Ltd. Animals were housed in a specific pathogen-free room at a temperature of 25˚C, a humidity of 45-50% and a 12 h light/dark cycle. All animals had free access to food and water, and were allowed to acclimatize to laboratory conditions for 7 days. All animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals and were approved by the Ethics Committee of Renmin Hospital of the Wuhan University.

Colitis was induced in C57BL/6 mice following treatment with 3% DSS [molecular weight (MW), 35,000-50,000 Da; MP Biomedicals, LLC] dissolved in drinking water for 7 days, as previously described (19). The progression of DSS-induced colitis was monitored daily by measuring the disease activity index (DAI), as previously described (19). A freeze-dried powder containing CB bacteria of 5x10⁹ colony forming units (CFU)/g was purchased from Kexing Biopharm Co., Ltd.

Experimental design

Mice were randomly divided into three experimental groups (n=15 mice/group: Control, DSS and DSS + CB groups. In the DSS and DSS + CB mice groups, animals were treated with 3% DSS dissolved in drinking water for 7 days, while mice in the control group received standard laboratory water ad libitum. During the induction of colitis, mice in the DSS + CB group were co-treated daily with 200 µl (2x10⁸ CFU) CB solution via gavage, as previously described (20). Mice in the control and DSS groups received 200 µl normal saline via gavage. All mice were anesthetized with pentobarbital solution (50 mg/kg) via intraperitoneal injection 7 days after standard laboratory water or DSS treatment. Following anesthesia, 0.5 ml blood was collected from the mice via eyeball enucleation. To isolate colon tissue, mice were sacrificed by cervical dislocation. Following a 2 min observation, mice were confirmed to be dead without respiration and corneal reflex. A 0.5-cm segment of the distal colon was cut and isolated, rinsed with PBS, fixed for 48 h in 4% (w/v) paraformaldehyde at 4˚C, dehydrated and embedded in paraffin.

Intestinal permeability assay

The intestinal permeability of mice with DSS-induced colitis was measured as previously described (21). A total of 44 mg/100 g body weight FITC-labeled 4-kDa dextran (FD-4; MW, 4,000 Da; Sigma-Aldrich; Merck KGaA) was administrated to each mouse via gavage following fasting for 4 h. Following FD-4 administration for 5 h, 400 µl blood was collected and centrifuged at 2,500 x g for 10 min at 4˚C to isolate serum. The serum fluorescence intensity was determined using a multimode reader (PerkinElmer, Inc.) at excitation wavelength of 485 nm and emission wavelength of 520 nm. Subsequently, a standard curve was generated using serial dilutions of FD-4.

Histology

Acolon segment was cut and isolated, its length was measured and analyzed using histology methods. Rectum tissues were fixed for 48 h with 4% (w/v) paraformaldehyde at 4˚C, dehydrated and embedded in paraffin. Subsequently, the tissue samples were sectioned (thickness, 4 µm), stained with hematoxylin and eosin (HE) for 97 min at room temperature and observed under an light microscope, at X100 and X200 magnification. Intestinal inflammation grading was performed as previously described (22). Histologic score was assessed in a blinded manner by two independent researchers.

Western blotting

To investigate the protein expression levels of claudin-1, claudin-2, occludin, and ZO-1, and the activation status of the Akt/mTOR signaling pathway, total protein samples were extracted from murine colon tissues using radioimmunoprecipitation assay lysis buffer (cat. no. P0013B; Beyotime Institute of Biotechnology), containing phenylmethanesulfonyl fluoride (cat. no. P105539; Shanghai Aladdin Bio-Chem Technology Co., Ltd.) and phosphoesterase inhibitors (cat. no. S1873; Beyotime Institute of Biotechnology). The concentration of the extracted proteins was measured using a bicinchoninic acid protein assay kit (Beyotime Institute of Biotechnology). Subsequently, total protein extracts were dissolved in 5X SDS-PAGE loading buffer and heated in a boiling water bath for 10 min. A total of 40 µg proteins/lane were loaded onto an SDS-PAGE (10% gel) and electrophoresis was performed for 1.5 h prior to protein transfer onto PVDF membranes. Following transfer, membranes were blocked with 5% skimmed milk in TBS-0.1% Tween 20 (TBST) buffer for 2 h at room temperature and then incubated with primary antibodies overnight at 4˚C. The following primary antibodies were used: Rabbit polyclonal anti-claudin-1 (cat. no. AF0127; 1:1,500), anti-claudin-2 (cat. no. AF0128; 1:1,500), anti-occludin (cat. no. DF7504; 1:1,500), anti-ZO-1 (cat. no. AF5145; 1:1,000; all from Affinity Biosciences, Inc.), anti-total-Akt (cat. no. 10176-2-AP; 1:1,000; ProteinTech Group, Inc.), anti-phosphorylated (p)-Akt (cat. no. 4060p; 1:1,000), anti-total-mTOR (cat. no. 2983; 1:1,000), anti-total-S6K (cat. no. 2708; 1:1,000), anti-p-S6K (cat. no. 9234; 1:1,000; all from Cell Signaling Technology, Inc.), anti-GAPDH (cat. no. AB-P-R001; 1:1,000; Hangzhou Goodhere Biotechnology Co., Ltd.). After washing five times with TBST for 5 min each, membranes were further immunoblotted with an HRP-conjugated AffiniPure goat anti-rabbit IgG (cat. no. BA1054; 1:50,000; Wuhan Boster Biological Technology, Ltd.) secondary antibody for 2 h at 37˚C. Subsequently, membranes were washed again with TBS-T for five times (5 min/time), treated with an ECL plus kit (cat. no. P1050; Applygen Technologies Inc.) for ~5 min at room temperature and the grayscale values of each band on the blots were analyzed with the BandScan v5.0 software (Glyko Biomedical Ltd.).

Reverse transcription-quantitative PCR (RT-qPCR)

The mRNA expression levels of claudin-1, claudin-2, occludin and ZO-1 were determined using RT-qPCR. Colon tissues were homogenized and total RNA was extracted with TRIzol^® reagent (cat. no. 15596-026; Ambion; Thermo Fisher Scientific, Inc.). Subsequently, total RNA was reverse transcribed into cDNA using a Hiscript RT reagent kit (cat. no. R101-01/02; Vazyme Biotech Co., Ltd.) under the following conditions: 25˚C for 5 min, 50˚C for 15 min, 85˚C for 5 min and 4˚C for 10 min. The qPCR step was performed using a SYBR Green Master Mix kit (cat. no. Q111-02; Vazyme Biotech Co., Ltd.), according to the manufacturer's instructions, on a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) under the following conditions: 50˚C for 2 min, 95˚C for 10 min; 40 cycles of 95˚C for 30 sec and 60˚C for 30 sec. The data was analyzed with the 2^-ΔΔCq method (23). GAPDH served as the endogenous control. The primer sequences and corresponding amplicon sizes are listed in Table I.

Table I.

List of PCR primers and amplicon size.

Gene name	Primer	Sequence	Size, bp
Mus GAPDH	Forward	5'-ATGGGTGTGAACCACGAGA-3'	229
	Reverse	5'-CAGGGATGATGTTCTGGGCA-3'
Mus claudin-1	Forward	5'-GGCTGATCGCAATCTTTGT-3'	131
	Reverse	5'-TAATGTCGCCAGACCTGAA-3'
Mus claudin-2	Forward	5'-CCGTGTTCTGCCAGGATT-3'	199
	Reverse	5'-GCTGAGATGATGCCCAAG-3'
Mus occludin	Forward	5'-TGGATCTATGTACGGCTCAC-3'	203
	Reverse	5'-CCATCTTTCTTCGGGTTT-3'
Mus ZO-1	Forward	5'-CCAGCAACTTTCAGACCACC-3'	154
	Reverse	5'-TTGTGTACGGCTTTGGTGTG-3'

ZO-1, zona occludens protein 1.

ELISA

Murine blood (0.5 ml) was collected from the orbital venous plexus, as aforementioned, and centrifuged at 2,500 x g for 10 min at 4˚C. The supernatants were subsequently collected and stored at -20˚C. The cytokines, TNF-α (cat. no. E-EL-M0049c), IL-1β (cat. no. E-EL-M 0037c), IL-6 (cat. no. E-EL-M0044c), IL-10 (cat. no. E-EL-M0037c) and IL-13 (cat. no. E-EL-M0727c) (all Elabscience, Inc.), were quantitatively measured using ELISA (Multiskan MK3; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocols. The absorbance (optical density value) was measured on a microplate reader and the corresponding concentration of each sample was converted from the standard curve.

Statistical analysis

The experiments were repeated three times. Statistical analyses were performed using SPSS 20.0 software (IBM Corp.). Data are presented as the mean ± SD. All data were analyzed using mixed and one-way ANOVA and followed by Tukey's post-hoc test. P<0.05 was considered to indicate a statistically significant difference.

Results

CB ameliorates DSS-induced colitis

To investigate the therapeutic efficacy of CB treatment in DSS-induced colitis, the alterations in body weight of mice were detected. No significant changes in body weight were observed in the control group during the experiment (Fig. 1). However, mice in the DSS group exhibited significant weight loss from the 5th day, which was reversed following treatment with CB from the 6th day. Subsequently, to assess the symptoms of DSS-induced colitis, the DAI score was determined in each group (Fig. 1B). The DAI score in the healthy control group was ~0 during the 7-day experimental period. However, mice in the DSS and DSS + CB groups developed obvious symptoms of colitis, including activity reduction, diarrhea and rectal bleeding. These symptoms appeared at the 3rd day after colitis induction, reaching peak on the 7th day. Compared with the DSS group, mice in the DSS + CB group also exhibited a significantly lower DAI score from day 6. Furthermore, the colon length was significantly decreased in the DSS group compared with the control group; however, the colon length was significantly increased in the DSS + CB group compared with the DSS group (Fig. 1C and D). These findings indicated that CB administration effectively alleviated DSS-induced colitis.

Figure 1.

CB treatment alleviates intestinal inflammation in DSS-induced colitis. Effect of CB on (A) body weight and (B) DAI score, (C) colon length, which is quantified in (D), and (E) FD-4 permeability. Data are presented as the mean ± SD. ^*P<0.05 vs. control group; ^#P<0.05 vs. DSS group. IBW, initial body weight; CB, Clostridium butyricum; DSS, dextran sodium sulfate; DAI, disease activity index; FD-4, FITC-labeled 4-kDa dextran.

CB restores intestinal barrier injury in the DSS-induced colitis mouse model

FD-4 was used to assess the intestinal permeability and the effects of CB on intestinal barrier function. Mice in the DSS group demonstrated 6-fold increased FD-4 serum levels compared with those in the control group. This finding suggested that the intestinal barrier function was severely impaired in mice with DSS-induced colitis. Additionally, compared with the DSS group, serum FD-4 levels were 1.7-fold lower in the DSS + CB group (Fig. 1E). The aforementioned data indicated that CB improved the intestinal epithelial barrier function in DSS-induced colitis.

Effects of CB on colon histology in the DSS-induced colitis mouse model

HE staining demonstrated that CB ameliorated the histopathologic changes in DSS-induced colitis. In the control group, the colonic mucosa was intact, glandular cells were arranged orderly and no inflammatory cell infiltration in the normal crypts was observed. However, compared with the control group, HE staining in the DSS group revealed epithelial defects, destroyed crypts and extensive inflammatory cell infiltration. Furthermore, compared with the DSS group, in the DSS + CB group, the colonic mucosa injury was mild, with more intact glands and reduced number of inflammatory infiltrate cells apparent in the crypts (Fig. 2A). The histological score among the three groups was significantly different; the score was significantly increased in the DSS group compared with the control group; however, the score was significantly decreased in the DSS + CB group compared with the DSS group (Fig. 2B).

Figure 2.

Effects of CB on colon histology in the DSS-induced colitis mouse model. (A) Representative images of colon tissues from control, DSS and DSS+CB groups (original magnification, x200). (B) Histological scores of colon tissue. Data are presented as the mean ± SD. ^*P<0.05 vs. control group; ^#P<0.05 vs. DSS group. DSS, dextran sodium sulfate; CB, Clostridium butyricum.

Effects of CB on intestinal inflammation and oxidative stress

The ELISA results indicated that treatment with DSS significantly elevated the secretion of proinflammatory cytokines, including TNF-α, IL-1β and IL-13. However, the increased cytokine serum levels were reversed following co-treatment with CB. On the contrary, IL-10 secretion levels were attenuated in the DSS group compared with those in the control group, but its levels were increased in CB-treated mice. Moreover, compared with the control group, IL-6 secretion levels in the DSS group were significantly elevated, but there was no significant difference between the DSS and DSS +CB group (Fig. 3A).

Figure 3.

Effect of CB on cytokines secretion levels and oxidative stress indicators. (Α) Serum levels of TNF-α, IL-1β, IL-6, IL-10 and IL-13 in different groups. Levels of oxidative stress indicators, (B) SOD, (C) MDA and (D) GSH in different groups. Data are presented as the mean ± SD. ^*P<0.05 vs. control group; ^#P<0.05 vs. DSS group. CB, Clostridium butyricum; TNF-α, tumor necrosis factor α; IL, interleukin; DSS, dextran sodium sulfate; MDA, malondialdehyde; GSH, glutathione; SOD, superoxide dismutase.

The results demonstrated that CB treatment attenuated the DSS-induced alterations in lipid peroxidation and antioxidant enzymatic status in colon tissues. Therefore, following treatment with DSS, MDA levels were significantly increased, while those of GSH and SOD were decreased compared with the control group. However, co-treatment with CB significantly restored the levels of MDA and GSH, and the enzymatic activity of SOD (Fig. 3B-D).

Effects of CB on the expression levels of claudin-1, claudin-2, occludin and ZO-1

The effects of CB on the protein and mRNA expression levels of the TJ-related proteins, claudin-1, claudin-2, occludin and ZO-1, were assessed using western blot analysis (Fig. 4A) and RT-qPCR (Fig. 4B), respectively. The protein expression levels of claudin-1, occludin and ZO-1 were decreased, and those of claudin-2 were elevated in the DSS group compared with the control group. However, the expression levels of claudin-1, occludin, ZO-1 and claudin-2 were significantly reversed following co-treatment with CB. Consistent with the western blotting results, the relative mRNA synthesis findings obtained via RT-qPCR, indicated that CB increased the mRNA expression levels of claudin-1, occludin and ZO-1, and decreased those of claudin-2 (P<0.05).

Figure 4.

Effects of CB on the expression levels of claudin-1, claudin-2, occludin and ZO-1. (A) Protein expression levels of claudin-1, claudin-2, occludin and ZO-1 in each group were measured using western blot analysis. (B) mRNA expression levels of claudin-1, claudin-2, occludin and ZO-1 were determined using reverse transcription-quantitative PCR. Data are presented as the mean ± SD. ^*P<0.05 vs. control group; ^#P<0.05 vs. DSS group. ZO-1, zona occludens protein 1; CB, Clostridium butyricum; DSS, dextran sodium sulfate.

Effects of CB on the activation of the Akt/mTOR signaling pathway

Further experiments were performed to investigate whether CB promoted the expression levels of TJ-related proteins via the Akt/mTOR signaling pathway. The results demonstrated that in the DSS-induced colitis group p-Akt protein levels were significantly reduced compared with the control group; however, in the DSS+CB group, p-Akt protein levels were significantly increased compared with the DSS group (Fig. 5). Subsequently, the protein expression levels of p-mTOR and p-S6K, two downstream molecules of the Akt/mTOR signaling pathway, were detected. Mice in the DSS group exhibited significantly decreased p-mTOR and p-S6K protein expression levels compared with the control group. However, pretreatment with CB elevated both p-mTOR and p-S6K protein expression levels, and the p-mTOR/mTOR and p-S6K/S6K ratios compared with the DSS group (Fig. 5). These findings suggested that DSS inhibited the activation of the Akt downstream signaling molecules, p-mTOR and S6K, while CB restored the DSS-induced suppression of protein synthesis.

Figure 5.

Effect of CB on the Akt/mTOR signaling pathway. (A) Representative western blot analysis results of Akt, mTOR and S6K. (B) Comparison of semi-quantitative results. Data are presented as the mean ± SD. ^*P<0.05 vs. control group; ^#P<0.05 vs. DSS group. CB, Clostridium butyricum; DSS, dextran sodium sulfate; S6K, p70 ribosomal protein S6 kinase; p-, phosphorylated.

Discussion

UC and Crohn's disease are the two main forms of IBD which has been shown to have a complex genetic basis and is affected by several risk factors, including environmental factors, the intestinal microbiota and the host immune system (24). Moreover, increasing evidence has revealed that the impaired intestinal barrier is an important feature of colitis (25,26). The gut microbiota and the host immune system constantly communicate with each other to maintain gut homeostasis and host health (24). CB is a strictly anaerobic gram-positive bacterium, which produces acetic and butyric acid, two important components of SCFAs synthetized by gut microbiota (27). Furthermore, CB commonly exists in the intestines of humans and animals, and has been proved effective to treat intestinal inflammation (20,28,29). CB also serves an important role in providing nutrients for the host and maintaining the microbial balance in the intestine, while exhibiting a high tolerance to several antibiotics (30,31). Therefore, present study aimed to investigate the specific molecular mechanisms of CB in protecting the intestinal mucosal barrier function in DSS-induced colitis.

Currently, the DSS-induced colitis model remains the most widely used mouse model of colitis mimicking human UC due to its similarities in terms of symptoms and histopathological changes (19). In the present study, C57BL/6 mice were treated with 3% DSS for 7 days to establish the acute colitis mouse model. Colitis in the DSS-induced model was accompanied by several symptoms, including diarrhea, weight loss, hematochezia and histological damage, such as surface epithelial loss, crypt destruction and increased inflammatory cell infiltration. The present results demonstrated that co-treatment of DSS-treated mice with CB relieved colitis symptoms and protected intestinal barrier function. Determining body weight, DAI and histological grading score in mice in the DSS, DSS + CB and control groups identified that CB not only alleviated colitis-related symptoms, maintained body weight and reduced DAI score, but also prevented histological damage by decreasing neutrophil infiltration, promoting the restoration of epithelial cell damage and reducing the rectal histological score. Furthermore, FD-4 serum levels were significantly decreased in the DSS + CB group compared with the DSS group.

Proinflammatory cytokines are associated with the intestinal barrier dysfunction in UC. For example, it has been reported that TNF-α increases TJ permeability by modulating myosin light chain kinase (MLCK) gene expression via the NF-κB signaling pathway in Caco-2 cells (32). In addition, IL-1β enhances intestinal TJ permeability by regulating p38 kinase activation of activating transcription factor 2 (ATF-2) and via ATF-2 regulation of MLCK gene activity (33). IL-13 is considered an important effector cytokine in patients with UC, which may lead to epithelial barrier dysfunction by regulating epithelial apoptosis, TJs and restitution velocity (34). IL-10, an anti-inflammatory cytokine, serves a key role in the intestinal immune system and is closely associated with IBD. A previous study has shown that IL10^-/- mice spontaneously develop colitis, which may be alleviated by the administration of IL-10(35). Furthermore, a genome-wide association study in humans revealed that a single-nucleotide polymorphism in the IL-10 gene was closely associated with IBD (36). Kuhn et al (37), demonstrated in two murine models of bowel injury that IL-6 was induced soon after injury in multiple cell types, while its inhibition resulted in impaired wound healing. Additionally, these authors found that IL-6 mRNA transcripts were enriched within the surrounding area of colon perforation in human patients, thus suggesting that this IL-6-driven wound healing mechanism could be important in humans. Previous studies have reported that butyrate inhibits the secretion of TNF-α, interferon-γ, IL-12, IL-5 and IL-13 (38,39), while CB promotes IL-10 production via intestinal macrophages in the inflamed mucosa of mice with experimental colitis (28). In the present study, treatment with CB also inhibited the expression levels of the proinflammatory cytokines TNF-α, IL-1β and IL-13, and promoted that of IL-10. Consistent with previous studies, the current results suggested that IL-6 secretion levels in mice with DSS-induced colitis were increased compared with the control group. However, the differences in IL-6 secretion levels between the DSS and DSS + CB groups were not significant, indicating that CB had no significant effect on IL-6 secretion. Therefore, the role of IL-6 in wound healing should be further investigated.

MDA, a marker for lipid peroxidation, is commonly used as an indicator for oxidative damage to cells and tissues (40). The present study demonstrated that in DSS-treated animals, MDA levels were increased, while co-treatment with CB significantly alleviated its levels. Furthermore, GSH, an important indicator of the body's antioxidant capacity, contributes to the antioxidant defense system (41). It has also been reported that GSH eliminates free radicals and protects the gastrointestinal mucosal epithelium by preventing injuries caused by inflammation, local ischemia and oxidants (42). SOD is an important antioxidant enzyme that attenuates tissue oxidative damage and acts as a free radical scavenger in defense against oxidative cell injury (43). In the current study it was identified that co-treatment of DSS-treated mice with CB significantly elevated GSH levels and SOD enzyme activities.

Inflammation and oxidative stress are two closely associated and tightly linked processes that provoke cell injury (44). It has been reported that the anti-inflammatory and antioxidative effects of CB may be associated with the metabolism of SCFAs. These molecules serve a critical role in colonic health by acting as important energetic substrates for epithelial cells (45), modulating immune cell adhesion molecules and affecting chemotaxis, cytokine release and leukocyte migration (46). In addition, SCFAs may modulate oxidative stress via inhibiting histone deacetylases (47). An in vitro study has shown that butyrate protects colonocytes from reactive oxygen species-induced DNA damage (48). The present study demonstrated that CB, as a major source of butyrate, inhibited the secretion of proinflammatory cytokines and reduced oxidative stress.

The present results suggested that CB could protect the intestinal barrier function via upregulating the expression of TJ-related proteins, including claudin-1, occluding and ZO-1, at both protein and mRNA levels. However, the mRNA and protein levels of claudin-2 were decreased in the DSS + CB group. Claudins serve an important role in intestinal barrier function and regulate the permeability of epithelial layers to ions and water by upregulating the expression of ≥1 of the 27 claudin isoforms (49). Each claudin molecule consists of four right helical folded transmembrane domains and two extracellular rings (50). The amino- and carboxyl-terminal groups reside in the cytosol and are linked to ZOs via the post-synaptic density 95/Drosophila discs large/zona-occludens 1 binding domain. Moreover, these two extracellular rings are the structural basis of TJs and affect the selective permeability to ions and water (50). Previous studies have reported that claudin-1, -3, -4, -5 and -8 exhibit sealing functions and protect the intestinal barrier function (51-55). Additionally, claudin-2, -7,-12 and -15 provide paracellular channels to promote the charge-selective passage of small ions and increase the permeability of intestinal mucosa (56-58).

Occludin regulates the localization and function of TJ complex via phosphorylation, which affects TJ permeability. Furthermore, two subtypes of occludin have been identified, characterized by the formation of two outer rings via four transmembrane domains; these extracellular rings are responsible for binding between adjacent cells (59). Amino acid residues in the intracellular domain of occludin directly bind to ZOs and subsequently interact with cytoskeleton proteins via cytoplasmic proteins (59). ZOs belong to the membrane associated guanylate kinase family and consists of three isoforms: ZO-1, -2 and -3. ZOs interact with several transmembrane proteins, and actin cytoskeleton and cytoskeleton-associated proteins via the N- and C-terminal regions, respectively (1). A previous study has revealed that CB upregulated ZO-1, claudin-1 and occludin expression levels in vitro in lipopolysaccharide (LPS)-treated IPEC-J2 cells and inhibited LPS-induced apoptosis (60). Moreover, Daly and Shirazi-Beeche (61) observed that butyrate downregulated claudin-2 expression in butyrate-treated colonic epithelial cells, using microarray analysis. Consistent with previous studies, the present results suggested that the protein and mRNA expression levels of claudin-1, occludin and ZO-1 were significantly increased in the CB-treated group. Furthermore, claudin-2 expression was higher in the DSS group compared with the control group, while CB could reverse this trend.

Akt, a serine/threonine kinase, is an essential intracellular signaling molecule that promotes cell survival, growth, proliferation and metabolism. It has also been reported that activation of Akt may lead to phosphorylation of mTOR, which in turn activates the downstream signaling molecules, S6K and 4E-BP1(62). Furthermore, activated S6K and 4E-BP1 directly regulate protein synthesis (63). Previous studies have revealed that the increased expression of TJ-related proteins in intestinal epithelial cells is closely associated with the activation of the Akt/mTOR signaling pathway (64). Yan et al (65) investigated the effect of butyrate on TJ permeability in a model of LPS-induced inflammation in IPEC-J2 cells, and found that butyrate elevated both mRNA and protein expression levels of TJ-related proteins via activating the Akt/mTOR-mediated protein synthesis. In addition, a recent study showed that microbiota metabolites, SCFA, activated the mTOR signaling pathway and promoted intestinal epithelial cell expression of RegIIIr and β-defensins in a G protein-coupled receptor 43-dependent manner (66). In the present study, p-Akt and total Akt protein expression levels were alleviated in DSS-treated mice, thus resulting in p-mTOR and p-S6K downregulation. This finding indicated that the Akt/mTOR signaling pathway was inhibited in DSS-induced colitis, and as a result, TJ-related protein synthesis was inhibited, leading to the disruption of the epithelial barrier function. However, treatment with CB restored p-Akt, p-mTOR and p-S6K protein expression levels, suggesting that CB could promote TJ-related protein synthesis via activating the Akt/m-TOR signaling pathway.

The current study has certain limitations. It remains to be investigated whether oral administration of CB increased the content of intestinal SCFAs or affected the gut microbiome. The safety of CB also requires further study. Moreover, animal studies have indicated that CB and SCFAs have a beneficial impact on colonic diseases; however, evidence in humans is currently lacking. Therefore, future studies are required to further determine the effects of combination therapy with anti-inflammatory drugs, prebiotics or probiotics in clinical trials. Due to funding constraints, the protective effect of CB on intestinal functional barrier will require further research.

In conclusion, previous studies have shown that butyrate exhibits anti-inflammatory, barrier-protective and anti-carcinogenic effects in colon. The present results further demonstrated that CB alleviated colon inflammation and restored intestinal barrier function in DSS-induced colitis, by promoting TJ-related proteins expression via the activation of the Akt/mTOR-mediated protein synthesis pathway.

Funding

This study was supported by the National Natural Science Foundation of China (grant no. 81800481).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

TD designed the experiments, interpreted and analyzed the data, and revised the manuscript. ML and WX designed and performed the experiments, and wrote the manuscript. XW was responsible for data and statistical analyses. All authors read and approved the final manuscript.

Ethics approval and consent to participate

All experiments in the present study were performed in accordance with the Guidelines on Animal Experiments from The Committee of Medical Ethics, The National Health Department of China. This study and all experimental protocols were approved by the Laboratory Animals Welfare and Ethics Committee of Renmin Hospital of the Wuhan University (approval no. 20190608).

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.