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[Preprint]. 2020 Oct 12:2020.10.07.20208520. [Version 1] doi: 10.1101/2020.10.07.20208520

Variability of Salivary and Nasal Specimens for SARS-CoV-2 Detection

Yukari C Manabe, Carolyn Reuland, Tong Yu, Razvan Azamfirei, Taylor Church, Diane M Brown, Thelio T Sewell, Justin P Hardick, Paul W Blair, Christopher D Heaney, Andrew Pekosz, David L Thomas
PMCID: PMC7553172  PMID: 33052355

Abstract

In a large cohort of ambulatory confirmed COVID-19 patients with multiple self-collected sample time points, we compared 202 matched nasal-oropharyngeal swabs and oral salivary fluid sample pairs by RT-PCR. Nasal-oropharyngeal swabs were more sensitive than this salivary sample type (oral crevicular fluid) suggesting that not all saliva sample types have equivalent sensitivity. However, all samples that were Vero E6-TMPRSS2 cell culture positive (e.g., infectious virus) were also oral fluid RT-PCR positive suggesting that oral fluid may find the patients most likely to transmit disease to others.

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