Figure 5.

Depletion of circulating monocytes abolishes memory deficit and gliosis induced by SNI (A) Recognition index accessed by NORT in mice in the sham group (Sh) and in groups 9 d after SNI with clodronate or vehicle (Vehi) injection are shown (n = 7 in vehicle and CLL sham groups, n = 8 in other groups). (B, C) Effect of clodronate on PECAM-1 expression and activation of microglia (CD11b) and astrocytes (GFAP) at 9 d after SNI. Scale bar = 100 µm. n = 3 mice/group, 3-4 images/mice, **P < 0.01, ***P < 0.001 vs. vehicle sham group, ^##P < 0.01, ^###P < 0.001 vs. vehicle SNI group, one-way ANOVA with Bonferroni's post hoc test (A) and two-way ANOVA with Bonferroni's post hoc test (C).