Targeting immunogenic cancer cell death by photodynamic therapy: past, present and future

Abstract

The past decade has witnessed major breakthroughs in cancer immunotherapy. This development has been largely motivated by cancer cell evasion of immunological control and consequent tumor resistance to conventional therapies. Immunogenic cell death (ICD) is considered one of the most promising ways to achieve total tumor cell elimination. It activates the T-cell adaptive immune response and results in the formation of long-term immunological memory. ICD can be triggered by many anticancer treatment modalities, including photodynamic therapy (PDT). In this review, we first discuss the role of PDT based on several classes of photosensitizers, including porphyrins and non-porphyrins, and critically evaluate their potential role in ICD induction. We emphasize the emerging trend of ICD induction by PDT in combination with nanotechnology, which represents third-generation photosensitizers and involves targeted induction of ICD by PDT. However, PDT also has some limitations, including the reduced efficiency of ICD induction in the hypoxic tumor microenvironment. Therefore, we critically evaluate strategies for overcoming this limitation, which is essential for increasing PDT efficiency. In the final part, we suggest several areas for future research for personalized cancer immunotherapy, including strategies based on oxygen-boosted PDT and nanoparticles. In conclusion, the insights from the last several years increasingly support the idea that PDT is a powerful strategy for inducing ICD in experimental cancer therapy. However, most studies have focused on mouse models, but it is necessary to validate this strategy in clinical settings, which will be a challenging research area in the future.

Introduction

The proper functioning of the immune system has a pivotal role in prevention of cancer initiation, progression and therapy. The role of the immune system in cancer therapy has been widely studied, and the modern paradigm of anticancer therapy has accepted the notion that interaction of dying/dead cancer cells with immune cells is a crucial factor determining cancer treatment efficiency. The Nobel Prize in Physiology or Medicine in 2018 reflects the significance of immunotherapy. The prize was awarded to James P. Allison and T. Honjo for revealing the specific molecular players in immune surveillance and formulating a strategy for using checkpoint inhibitors as a potential cancer therapy.1 2

Over the past decade emerged the concept of immunogenic cell death (ICD), a cell death modality that stimulates innate and adaptive immune responses resulting in the generation of long-term immunological memory.3–5 The immunogenicity of cancer cell death is dictated by the antigenicity and adjuvanticity of dying cancer cells.3 6 The antigenicity of tumor cells is determined by the presence of tumor-associated antigens (TAA) and tumor neoantigens (TNA). However, they usually fail to drive efficient immunity in the absence of additional adjuvants required for the recruitment and activation of antigen-presenting cells (APC). ICD has an adjuvant-like effect mediated by the release of damage-associated molecular patterns (DAMPs). These molecules are normally retained within cells and integrated in their normal functioning, but once released outside the cells, they act as danger signals.7 8 DAMPs can be actively secreted, passively released extracellularly or exposed on the dying cell surface. It is believed that emitted DAMPs promote the recruitment and maturation of APCs (eg, dendritic cells) and thereby mediate presentation of TAA and TNA to effector CD8 T cells. The list of DAMPs is still expanding and includes calreticulin (CRT), heat shock proteins (HSPs) 70 and 90, high-mobility group box 1 (HMGB1), ATP, annexin A1, type I interferons (IFNs) and mitochondrial DNA.3 9 10 These molecules differ in origin, function, cell localization, release mechanism and stage of death at which they are released.11–13 The ability of cancer therapy to induce ICD is clinically important because ICD stimulates anticancer immune responses that are critical for the efficacy of the therapy and long-term anticancer immunity.14–17

Recently, much attention has been given to ICD, which can be induced by different stimuli and anticancer treatment modalities, including chemotherapy with anthracyclines and oxaliplatin, radiotherapy, UVC irradiation, oncolytic viruses and photodynamic therapy (PDT).4 10 15 18 19 The ICD induced by various stimuli can differ in the DAMPs’ profile and has also been linked to different cell death modalities such as apoptosis, necroptosis20–22 and ferroptosis.23 24 In this review, we first discuss the role of PDT in the induction of ICD and then assess the advantages and disadvantages of PDT in the induction of ICD. Finally, we discuss possible strategies for enhancing the ICD-inducing potential of PDT-based anticancer therapies.

Main principles of PDT

PDT of cancer involves the systemic, local or topical administration of a non-toxic, light-sensitive dye known as a photosensitizer (PS). After the PS accumulates selectively in the tumor, it is excited by illumination with visible light of appropriate wavelength. In the presence of molecular oxygen in cells and tissues, this leads to the generation of cytotoxic species and stimulation of signaling pathways, which consequently leads to cell death and tumor tissue destruction.25 PDT was first applied in the clinic in 1903 (box 1) and then it became widely used to treat several types of cancer.25–29 It is noteworthy that PDT is currently also used to treat some autoimmune30 and infectious diseases.31 32 Very recently, cetuximab saratolacan was approved by the Japanese government for the treatment of locally advanced or recurrent head and neck cancer.33 This is the first PS conjugated to an antibody; it consists of the water-soluble silicon-phthalocyanine derivative, IRDye700DX (IR700), conjugated to cetuximab. Cetuximab, which is approved by the FDA, targets the epidermal growth factor receptor, which is overexpressed in many types of cancer. In this treatment, after cetuximab saratolacan is injected intravenously, it attaches to head and neck cancer cells expressing high levels of EGFR. Subsequent illumination with red light (690 nm) as part of the PDT leads to induction of ICD in tumors and a potent anticancer immune response.

Box 1. Historical background of photodynamic therapy (PDT): from fundamental studies to clinical practice.

Discovery and development of PDT

A mechanism discovered in 1900 in Munich, Germany by Oscar Raab, who worked under the supervision of Professor Herman von Tappeiner, laid the basis of PDT. Studies on the effect of different dyes on protozoan viability helped him to notice that light irradiation of infusoria in the presence of acridine red dye leads to infusoria’s death. Interestingly, the observed effect was more pronounced in comparison with light irradiation alone and with the dye action in the dark. Oscar Raab and Hermann von Tappeiner initially linked this phenomenon to light energy transfer to the dye, similar to photosynthesis.110 Dr H. Tappeiner published research,111 in which he first suggested the possibility of using the photodynamic effect for medical purposes (the historical name of the mechanism is associated with light action on the dynamics—mobility—of cells; the term was introduced in 1907).

In 1907, Dr Jodlbauer and Dr Tappeiner proved that the development of photodynamic reactions requires the presence of oxygen in their environment.112

PDT in clinical practice

The use of the photodynamic effect in practice started only a few decades later. In 1948, Figge summarized a series of studies showing that exogenously injected porphyrins can selectively accumulate in murine tumors.113 In these years, suggestions emerged for the possibility of using porphyrins to detect malignancies in the body. In 1955, Schwartz obtained a purified mixture of hematoporphyrins known as hematoporphyrin derivative (HpD), the first generation of photosensitizers. In 1978, Thomas Dougherty’s team (Roswell Park Cancer Institute, Buffalo, New York, USA) used HpD to treat tumors of various localizations.114 Later, in 1980, Dougherty synthesized from HpD the drug Photofrin, a mixture of hematoporphyrin oligomers connected to each other by ester and complex ester linkages. At the same time, Photofrin analogs were obtained in different countries, including Photosan (Germany), Photogem (Russia), Hiporfin and Deuteporfin.115–117 Since the 1980s, there has been a rapid development of PDT, including the development of new drugs and capabilities for their application. Photosensitizers of different chemical nature are being developed,118 and areas of PDT application are expanding: anticancer therapy, acne,119 antimicrobial therapy,32 psoriasis,120 atherosclerosis,121 herpes122 and age-related macular degeneration.123

The photodynamic reaction during PDT is based on photophysical and photochemical processes (figure 1). After absorption of light (photons), the PS in its ground state is activated to the short-lived (nanoseconds) excited singlet state ¹PS^·, after which it loses its energy by emitting light (fluorescence) or by internal conversion into heat. The excited singlet state ¹PS^· may also undergo the process known as intersystem crossing to form the relatively long-lived (microseconds) excited triplet state ³PS^·. The excited triplet state ³PS^· can then undergo two kinds of reactions with surrounding molecules.

Figure 1.

Mechanisms of photodynamic reaction during photodynamic therapy (PDT). (1) Following the absorption of photons (hv), one of the electrons of the photosensitizer (PS) is boosted into a high-energy orbital (S₁ or S₂) and activated to the short-lived (nanoseconds) excited singlet state (¹PS^·). ¹PS^· can lose its energy by internal conversion into heat (2) or by emitting light (fluorescence) (3). Alternatively, ¹PS^· transforms into a relatively long-lived (microseconds) excited triplet state (³PS^·) via an intersystem crossing process (4). ³PS^· moves directly from a triplet to a singlet state (¹PS) by emission of light (phosphorescence) (5) or undergoes two kinds of reactions with surrounding molecules. In the type I photochemical reaction (6), ³PS^· reacts directly with a substrate (eg, polyunsaturated fatty acids in cell membrane lipids) and transfers an electron or a proton, forming organic radicals. These radicals may further react with cellular oxygen to produce reactive oxygen species (ROS), such as superoxide anion (O₂^–·), hydroperoxide radical (HOO^·), peroxides (H₂O₂, ROOH) and hydroxyl radical (HO^·), as well initiate free radical chain reactions. In the type II photochemical reaction (7), the triplet ³PS^· can undergo triplet−triplet energy transfer to molecular oxygen (triplet in the ground state) to form excited-state singlet oxygen (¹O₂). Type I and type II photochemical reactions can be simultaneous, and the ratio between them depends mainly on the type of PS used, the concentrations of substrate and the availability of oxygen. As a result of the photodynamic reaction, various molecular mechanisms are activated, leading to different cell death modalities, recruitment and activation of immune cells and vascular damage.

In the type I photochemical reaction, ³PS^· reacts directly with a substrate, such as polyunsaturated fatty acids in cell membrane lipids, and transfers an electron or a proton, leading to the formation of organic radicals (figure 1). These radicals may further react with cellular oxygen to produce reactive oxygen species (ROS) such as superoxide anion (O₂^–·), hydroperoxide radical (HOO^·), peroxides (H₂O₂, ROOH) and hydroxyl radical (HO^·), and initiating free radical chain reactions. The hydroxyl radical, HO^·, forms predominantly in the reaction of peroxides with Fe²⁺ (Fenton reaction). HO^· is the most active oxygen radical, lives no more than hundreds of nanoseconds, and can oxidize almost any organic molecule. Alternatively, in the type II photochemical reaction, the triplet ³PS^· can undergo triplet−triplet energy transfer to molecular oxygen (triplet in the ground state) to form excited-state singlet oxygen (¹O₂), an extremely strong oxidizing agent with a lifetime in biologic media from a few to hundreds of nanoseconds (figure 1). Type I and type II photochemical reactions can occur simultaneously, and the ratio between them depends mainly on the PS type, substrate concentrations and oxygen availability. However, for example, when using tetrapyrrolic PS in a cellular environment, type II photochemical reactions prevail, and ¹O₂ is regarded as the most important ROS in PDT-mediated cytotoxicity. The primary products of the interaction of ¹O₂ with biological molecules (including lipids, proteins and nucleic acids) are hydroperoxides and cyclic endoperoxides, the decomposition of which, as in type I photodynamic reactions, initiates chain reactions of free radical peroxidation.

PSs can be divided according to chemical structure into non-porphyrin and porphyrin (or tetrapyrrole) compounds. The most common non-porphyrin PSs are based on phenothiazine dyes (analogs of methylene blue and toluidine blue), cyanines such as merocyanine 540 and polycyclic aromatic compounds, including hypericin and hypocrellin. PSs with a tetrapyrrole structure are more common. The first clinically approved PSs are hematoporphyrins (HpD, eg, Photofrin) (box 1), which are still being used in the clinic, for example, for treatment of cancer of the cervix,34 esophagus,35 colorectal cancer36 and oral squamous cell carcinomas (SCC).37 Efforts to reduce the skin toxicity of PSs optimize their optical and physico-chemical properties and improve their selective accumulation in tumors led to the production of numerous second-generation photoactive dyes. Active substances that have been clinically approved or are being preclinically tested as second-generation PSs are from the groups of texafirins (Lutrin), phenylporphyrins (m-THPP), chlorins (NPe6, Foscan, Verteporfin, Radachlorin, Photodithazine), bacteriochlorins (Tookad) and porphyrazines (Photosens, Photocyanine, Pc4). Besides this, 5-aminolevulinic acid and its derivatives, which are low-molecular-weight prodrugs, are precursors of endogenous protoporphyrin XI in the heme biosynthetic pathway. To further improve the pharmacokinetics of PSs and thereby reduce their systemic side effects, so-called third-generation PSs are being proposed by various research groups. The main idea of third-generation PSs is based on combining a photoactive chromophore with a targeting moiety or vehicle for directed delivery to cancer cells. In recent years, nanotechnology has been used for this purpose, including polymeric nanoparticles, micelles, nanostructured lipid carriers, liposomes and metal nanoparticles. In addition, targeted PS delivery is also being developed by using the technology for dendrimer preparation and conjugation of PSs with biomolecules, including sugars actively captured by tumor cells or proteins effectively binding to receptors that are hyperexpressed on the tumor cell surface.38 39

The radical ¹O₂, the photochemical production of which underlies the photodynamic effect of almost all PSs, has a short lifetime. Therefore, it is important that PS localization in tumor tissue occurs between PS administration and irradiation (drug-to-light interval). The production of ROS induced by PDT leads to tumor destruction by various mechanisms, depending on the localization of the particular PS. PDT influences the tumor vasculature, causing shutdown of vessels and consequently depriving the tumor of oxygen and nutrients.40 Equally important is the rapid recruitment and activation of immune cells, which leads to tumor elimination and long-term tumor control41 42 (box 2). Importantly, PDT can also directly induce ICD of tumor cells by irreversible light-driven damage, which will be discussed further in this review (figure 2).

Box 2. Role of the photodynamic therapy (PDT) in the modulation of anticancer immunity.

Several studies revealed that PDT effectively modulates both innate and adaptive immunity. Local injuries and oxidative stress in the tumor tissue induced by PDT activate an acute inflammatory process necessary to remove tissue residues and restore homeostasis (direct pathway). On the other hand, immunogenic cell death (ICD) induced by PDT leads to activation of antitumor immunity through danger signaling mechanisms caused by activation of damage-associated molecular patterns (DAMPs), which stimulate innate immunity, resulting in activation of adaptive immune responses (indirect pathway).124

The participation of the immune system in the development of the organism’s response to photodynamic effects (direct pathway) was first mentioned in a paper by Yamamoto et al in 1991, who described the activation of macrophages (mediated by Fc-receptors) due to lipid peroxidation of lymphocyte membranes under the action of reactive oxygen species generated by photodynamic reactions.125 In 1993, Agarwal et al showed that PDT causes rapid and massive release of proinflammatory mediators from the membranes of tumor cells, damaged endothelial cells and tumor stroma cells.126 127 In 1996, Korbelik revealed the induction of inflammatory mediators during PDT, such as arachidonic acid, cytokines, histamine and the complement system.128

In the same period, the works of Gollnick et al and Nseyo et al were the first to mention that PDT-treated cells secrete a number of cytokines, including tumor necrosis factor, interleukin (IL)-1β and IL-6, which participate in the recruitment of neutrophils and other myeloid cells.129 130 A few years later, Gollnick et al demonstrated that tumor cell lysates obtained after PDT can activate dendritic cells and induce an antitumor immune response (indirect pathway).131 A decade later, in 2012, the team led by P Agostinis used a ‘gold standard’ model of immunocompetent mice vaccinated with PDT-treated cancer cells to demonstrate for the first time the immunogenic nature of PDT-induced tumor cell death.43

Figure 2.

Photodynamic therapy (PDT)-induced immunogenic dell death at a glance. Photosensitizers (PSs) used in PDT have various chemical structures and can be divided into non-porphyrin and porphyrin (or tetrapyrrole) compounds. It has been experimentally proven that after accumulation in tumor cells and excitation by light of appropriate wavelength (hv), some PSs in each group of PSs can induce immunogenic cell death (ICD) (1). ICD refers to an immunological feature of cell death and is observed in immunogenic apoptosis and immunogenic necroptosis, as well as in mixed cell death types (2). The role of PDT in the induction of ferroptosis 132 133 in cancer cells needs to be further clarified 134. Importantly, only a fraction of cancer cells can be reached by light during PDT because light can penetrate only to a limited depth. ICD stimulates innate and adaptive immune responses, resulting in long-term immunological memory. Of note, the immunogenicity of ICD is mediated by the antigenicity (3) and adjuvanticity (4) of dying/dead cancer cells. The antigenicity of tumor cells is determined by the presence of tumor-associated antigens and tumor neoantigens (3). However, tumor-associated antigens usually fail to drive efficient immunity in the absence of additional adjuvants required to recruit and activate antigen-presenting cells. It is currently not known how PDT in combination with the above-mentioned PSs can modulate the antigenicity of dying cancer cells. The adjuvanticity of ICD resides in the release of damage-associated molecular patterns (DAMPs) such ATP, HMGB1 and HSP and CRT exposure on the outer cell surface (4). The emitted DAMPs promote the recruitment and maturation of antigen-presenting cells (eg, DCs) (5, 6), which leads to optimal antigen presentation to CD8⁺ T cells (7) and induction of antitumor immunity (8), resulting in significant suppression of tumor growth and/or regression of cancer and decreased risk of metastasis. The activated anticancer immunity aims to eradicate cells deep within the primary tumor and, therefore, significantly enhance PDT efficiency. The ‘gold standard’ for determining the true immunogenicity of cell death requires the conduction of experimental studies in vivo (mouse prophylactic tumor vaccination model) (9). For this, immunocompetent mice are first vaccinated with PDT-treated cancer cells in one flank and 1 week later rechallenged with living cells of the same type in the other flank (10). Protection against tumor growth at the challenge site is interpreted as a sign of successful priming of the adaptive immune system (11). *Examples of PSs with presumed but not fully proven immunogenic properties (lack of DAMPs expression and/or lack of immunogenicity either in vitro or in vivo). CD, cluster of differentiation; CRT, calreticulin; DC, dendritic cell; HMGB1, high-mobility group protein box 1; HSP, heat shock protein; hv, photons, IFN, interferon; IL, interleukin;.

PS features: subcellular localization and dose

Depending on their chemical properties, PSs can accumulate and initiate their damage in different cellular compartments. The main sites of PS localization are mitochondria, lysosomes, the endoplasmic reticulum (ER), Golgi apparatus, plasma membrane or their combinations. As localization plays an important role in determining whether the cell death will be immunogenic, characterization of new PSs should include analysis of their subcellular localization sites. It has been shown that one of the prerequisites of ICD is ROS production induced by ER stress, with subsequent exposure of one of the key DAMPs, CRT and activation of the host immune system against cancer.19 43 Therefore, from the point of view of PDT-induced ICD, it is logical to assume that direct targeting of PSs into the ER will be an effective strategy for cancer eradication. For instance, some studies have demonstrated that hypericin directly accumulates in the ER, and on PDT, it leads to the production of high levels of ROS and subsequent formation of strong immune responses.43 44 However, not all PSs accumulate in the ER. For a PS to accumulate in the ER, it must have hydrophobic or amphiphilic properties, and in the latter case, it also depends on the charge of the PS molecule. Hydrophilic PSs localize primarily in endosomes/lysosomes and are then redistributed in the cytoplasm. If the PS is delivered directly into the ER, both PDT efficiency and immunogenic effects increase.43 Several studies implemented this strategy by double-targeting the ER to trigger robust ER stress. For instance, a nanosystem for synchronous ER-targeting PDT immunotherapy has been developed. The first part of the system ensures delivery of the PS indocyanine green by conjugating it with hollow gold nanospheres and FAL peptides acting as targets for the ER. The second part consists of FAL-modified liposomes linked to hemoglobin as an adjuvant oxygen supply. Although indocyanine green localizes mainly in the cytoplasm, its targeted delivery into the ER in combination with enhancement of oxygen availability induces robust ROS-based ER stress followed by CRT exposure, DC maturation (CD11c⁺CD80⁺CD86⁺), stimulation of CD4⁺ and CD8⁺ T cell proliferation and production of cytotoxic cytokines (tumor necrosis factor (TNF)-α, IFN-γ) in CT26 and B16 tumor models.45 The ER involvement in the development of an immunogenic response was shown for photodithazine-based PDT against murine glioma GL261 and murine fibrosarcoma MCA205 cells.46 These findings indicate that redirecting the PS to localize in the ER promotes effective PDT-induced cancer cell death, followed by development of an adaptive antitumor immune response.

On the other hand, localization of the PS in other cellular compartments may also have immunogenic properties when used in PDT. In this regard, it has been shown that while photosens is localize mainly in lysosomes, its immunogenicity in PDT has been demonstrated. This immunogenicity is characterized by DAMPs emission (CRT, HMGB1 and ATP), DC maturation and effective decrease of tumor growth in the fibrosarcoma MCA205 murine prophylactic tumor vaccination model.46

Moreover, it is conceivable that PSs can simultaneously affect several cellular compartments. For instance, redaporfin specifically accumulates in the ER and Golgi apparatus and induces apoptosis.47 Dispersion of the Golgi apparatus or inhibition of its function significantly reduces the efficiency of redaporfin-based PDT. In the PDT reaction in a prophylactic tumor vaccination model using PDT-treated TC1 lung cancer cells, redaporfin acts as an ICD inducer that triggers eIF2a phosphorylation, DAMPs release (ATP, CRT, HMGB1) and decreased tumor growth.47

Of interest is that an approach that damages both lysosomes and mitochondria can be achieved by the simultaneous application of two PSs. In PDT, this approach has been realized by using the following pairs of PSs: Photofrin or N-aspartyl chlorin E6 (NPe6), which target lysosomes, and a benzoporphyrin derivative (BPD, Verteporfin), which targetets mitochondria.48–50 In contrast to the use of a single PS, this PDT protocol sequentially evoked lysosomal and then mitochondrial photodamage, which provided better tumor eradication. However, the question of whether this approach can activate ICD has not been raised. Sequential application of two PSs with different subcellular localization is a promising ICD-inducing strategy, and as greater understanding is gained, many more interesting and challenging findings are expected.

In addition to the localization characteristics of PSs, it is important to mention that high doses of PSs increases the risk of side effects (eg, pain, erythema, non-scarring skin lesions and death of non-tumor cells in the vicinity of the light-exposed area).51 Therefore, it is important to select an optimal PS dose at which PDT induces ICD with minimal damage to normal cells. The efficacy of ICD induction during PDT may be non-linearly dependent on the PS dose.

It has been shown that a low dose of the non-porphyrinic PS OR141 in PDT induces a slower death of mouse SCC7 and human A431 SCC cells, but it provides a more rapid emission of DAMPs (HMGB1, ATP, annexin A1, Hsp90) and expression of major histocompatibility complex (MHC) I molecules (H2Kk). The subsequent inhibitory effects of the low dose on tumor growth in immunocompetent C3H/HeNRj mice was more pronounced than when a 10-fold higher dose was used.52 PSs might also be able to penetrate into normal cells, so the use of high PS doses can cause significant dark toxicity to normal non-cancerous cells. This can be detrimental for several cell types, and particularly brain cells, as morphofunctional disorders in neuron-glial networks may lead to significant disruption of central nervous functions and aggravate the patient’s condition.53 54

The use of high doses of PS can be avoided by using nanostructures to deliver the PS directly to specific tumor cell compartments to trigger ICD while minimizing contact with normal cells. Therefore, to improve the efficiency of the PDT reaction, there is a need to develop nanosystems with an optimal combination of PSs and ICD inducers that will maintain the oxygen supply at the target location.

Classes of photosensitizers in PDT-induced ICD

ICD refers to an immunological characteristic of cell death that does not correlate with other features, including mechanism and manifestations, and can involve several cell death modalities (apoptosis, necroptosis, ferroptosis, pyroptosis) that stimulate a host immune response against antigens derived from dying/dead cancer cells.19 20 22 24 55 56 The ability of anticancer treatment to efficiently trigger ICD is one of the key prerequisites for successful anticancer therapy. Although many cellular stressors can induce ICD, the specific pattern of the molecular players and particular death mechanisms depend on the treatment modality and probably on the cancer cell type as well.57 For cancer cells to be considered immunogenic, the following criteria must be fulfilled.4 19 58 First, the cancer cells undergoing ICD in vitro must stimulate immune responses that protect mice against challenges with live tumor cells, that is, that they function as a vaccine. In this way, in vivo ICD must trigger a response of the innate and subsequently adaptive immune system that lead to suppression of tumor growth at the site rechallenged with cancer cells. Of note, APCs preloaded with dying/dead cancer cells can also be employed for vaccination.59 Thus, the concept of ICD implies activation of the innate and adaptive immune system components by actively or passively emitted DAMPs. Although the emission of DAMPs from PDT-treated cancer cells has been widely reported, the pattern of DAMPs varies depending on the treatment regimen and the type of cancer cells. The most universal feature of dying PDT-treated cancer cells is exposure of the calcium-binding protein CRT on the outer surface of the plasma membrane.43 52 60 61 CRT is normally localized in the ER lumen, but when it is exposed on the surface, it is recognized by low-density lipoprotein receptor-related protein 1 (LPR1, CD91) and serves as the ‘eat me’ signal for APCs. CRT exposure is attributed to ER stress caused by accumulation of misfolded proteins and the resultant unfolded protein response (UPR).43 62–64 It should be noted that the detailed molecular mechanisms of the PDT-induced CRT exposure can differ depending on the type of PS. For example, CRT exposure on Rose Bengal acetate (RBA) treatment is accompanied by co-translocation of ER protein 57,65 whereas during hypericin-based PDT this co-translocation is not detected.43 66 Also, the phosphorylation of eukaryotic initiation factor 2α plays a crucial role in UPR induction and is commonly regarded as obligatory for CRT exposure.67 However, this phospohorylation can be absent on hypericin-based PDT.43 66 Interestingly, CRT can also be released by activated macrophages, on which it can bind to the surface of viable cells and thereby mediate their clearance.68 69

Other ER chaperones can also be exposed on the plasma membrane surface of dying cancer cells. PDT-induced externalization has been reported for HSPs, including HSP70, HSP90, HSP27, HSP34, HSP60 and HSP72/73.43 65 66 70–74 It is known that these DAMPs are required for presentation of TAA to APC, thus promoting the anticancer immune responses.

Other reported PDT-associated DAMPs include HMGB1 and ATP, which are emitted from PDT-treated dying/dead cells.46 70 75 Recently, it has been shown that photodithazine and photosens are also capable of inducing ICD associated with ATP and HMGB1 emission.46 HMGB1 can induce activation of innate immune responses by interaction with toll-like receptors 2 and 4 and possibly with other pattern recognition receptors on APC. However, ATP promotes the recruitment of APC by binding to their purinergic receptors, which is interpreted by APCs as a ‘find me’ signal. ATP can be either passively released from cells because of the loss of plasma membrane integrity or actively secreted; in the latter case, it is regulated by a specific signaling pathway.19 Like the CRT mechanism, the precise mechanism of ATP release under PDT treatment seems to have its specific features. For example, in contrast to the ICD induced by chemotherapeutics,76 hypericin-based PDT induces secretion of ATP in an autophagy-independent manner.77 All these data suggest that PDT based on different PSs efficiently induces the emission of the key DAMPs from cancer cells (figure 2).

Another factor that may modulate anticancer immune responses to ICD, as well as ICD itself, is the PS dose and the light energy used for irradiation in PDT regimen. In fact, these two parameters affect the strength of cell death induction and determine how quickly cancer cells proceed to the late stages of cell death,24 which has been recently shown to be decisive in the immunogenicity of dying cancer cells. In this regard, it has been shown that the optimal radioimmunotherapy regimens are highly dependent on how the radiotherapy doses and fractionation schedules modulate type I IFN.78 79 Therefore, a better understanding of the correlation between ICD on the one hand and the PS dose and the light energy on the other will provide a deeper understanding of the molecular mechanism of immunogenicity. In turn, that will lead to the development of novel, more efficient therapeutic approaches that may have important implications for the choice of PDT regimens in the clinic to convert immunotherapy unresponsive patients into responders.

Non-porphyrin PSs in ICD induction

The first PS shown to induce ICD is hypericin, an anthraquinone derivative of natural origin with specific ER localization43 66 (figure 2). It is still the most studied PS, and the molecular mechanisms underlying its induction of ICD and subsequent development of ICD have been at least partially elucidated.43 66 70 In their initial work, Garg et al showed that in T24 human bladder carcinoma cells in vitro, hypericin-based PDT can induce ICD with surface exposure of HSP70 and CRT as soon as 30 min after PDT, and that this is associated with the active secretion of ATP and passive release of CRT, HSP90 and HSP70. Co-incubation of PDT-treated dead cells with JAWSII murine DCs resulted in their phenotypic maturation (CD80^high, CD83^high, CD86^high, MHC II^high) and functional stimulation (NO^high, IL-10^absent, IL-1β^high)43 64 66 (table 1). In vivo, they showed that cancer cells undergoing cell death after hypericin-based PDT are immunogenic in two different mouse models by using CT26 colon carcinoma cells and orthotopic glioma cells (GL261). The authors showed that dead/dying CT26 murine colon carcinoma cancer cells protected syngeneic mice against subsequent challenge with the same viable cell line,43 and the prophylactic efficacy of the ICD-based DC vaccine was demonstrated in the orthotopic GL261 murine glioma. It has been shown that the ICD-based DC vaccine induces an increase in brain infiltration with CD3⁺, CD4⁺ and CD8⁺ T-lymphocytes, Th1 cells, CTLs and Th17 cells, along with a significant reduction in regulatory T cells.70 Re-exposure of splenocytic T cells to untreated glioma cells led to enhanced IFN-γ production, which can be regarded as a sign of an immune memory response. These studies demonstrate that hypericin-based PDT efficiently induced ICD in several cancer models in vitro and in vivo.

Table 1.

ICD induction by non-porphyrin photosensitizers

PS	Subcellular localization of PS	Cell line	Markers of cell death and cell death types	Mode of DAMPs release/exposure	Immunogenicity of cancer cells in vitro	Immunogenicity of cancer cells in vivo	References
Hypericin	ER	T24 human bladder carcinoma	N/D	Surface exposure of HSP70 and CRT; no surface exposure of HSP90	N/D	N/D	66
		T24 human bladder carcinoma	Apoptosis (PtdSer exposure)	Surface exposure of CRT; No surface exposure of HSP90; release of ATP, CRT, HSP90, and HSP70	Phenotypic maturation of DCs (CD80high, CD83high, CD86high, MHC IIhigh) and functional stimulation (NOhigh, IL-10absent, IL-1βhigh)	N/D	43
		CT26 murine colon carcinoma	Apoptosis (WB: caspase-3 and PARP cleavage)	Surface exposure of CRT; release of ATP	N/D	Prophylactic vaccination model using hypericin-PDT-treated CT26 cells in immunocompetent BALB/c mice
		GL261 murine glioma	Apoptosis (WB: caspase-3 cleavage)	Surface exposure of CRT, HSP70 and HSP90; release of HMGB1 and ATP	Phenotypic maturation of DCs (CD80high, CD86high, CD40high, MHC Ihigh)	Prophylactic PDT-based DC vaccination model in immunocompetent, syngeneic C57BL/6 mice: ↑ brain infiltration by CD3+ T-lymphocytes, CD4+ and CD8+ T-lymphocytes, TH1 cells, CTLs and TH17 cells. Splenocytic T cells showed significantly higher IFN-γ production on restimulation with GL261 cells	70
OR141	ER	SCC7 murine head and neck carcinoma, A431 human epidermoid carcinoma	Necrosis and late apoptosis (WB: PARP cleavage, PtdSer exposure with a vital dye staining)	Surface exposure of CRT; release of HSP90, annexin A1, HMGB1 and ATP	Phenotypic maturation of DCs (CD80high, CD86high, MHC IIhigh)	Therapeutic vaccination model in C3H/HeNRj mice: PDT-based DC vaccination more effective than PDT-killed SCC7 cells vaccination. CD8+ infiltration in PDT-treated tumors	52
		Ab1, Ab12 murine mesothelioma	N/D	Surface exposure of CRT; release of HSP90 and HMGB1	Phenotypic maturation of DCs (CD80high, CD86high, CD40high, MHC IIhigh)	Therapeutic PDT-based DC vaccination model in BALB/c mice (Ab1 cells): ↑ infiltration with CD8+ T-lymphocytes, and INF-γ in tumor. ↑ migration potential of DCs primed with OR141-killed mesothelioma cells. ↑ CD8+ and CD4+T cells in splenocytes, ↑ IFN-γ production (IFN-γ positive CD8+ population) when re-exposed to Ab1	71
8-Methoxypsoralen (8-MOP), extracorporeal photochemotherapy 8-MOP, extracorporeal photochemotherapy	N/D	YUMM1.7 murine melanoma, MC38 murine colon adenocarcinoma	Apoptosis (APO2-PE, trypan blue, and/or PtdSer and PI staining135)	N/D	Phenotypic maturation of DCs (CD80high, CD83high, CD86high, MHC IIhigh, HLA-DRhigh) TI protocol using human PBMC: DCs activation and maturation (surface upregulation of the HLA-DR, CD80, CD83, and CD86), monocytes activation (↑ ICAM-1, PLAUR and CCL2+ cells)	Therapeutic vaccination model using mixture of PBMCs incubated with PDT-treated tumor cells in tumor-bearing C57BL/6J mice. Depletion of CD4 T cells, CD8 T cells and NK1.1+ cells in YUMM1.7-bearing mice diminished the antitumor effect of TI treatment. Prophylactic vaccination model using isolated splenocytes or enriched splenic T cells from TI-treated YUMM1.7-bearing mice	83 135
		B16 murine melanoma expressing ovalbumin (B16-OVA), YUMMER murine melanoma, MC38 murine colon adenocarcinoma	Apoptosis (PtdSer exposure)	Surface exposure of CRT, release of ATP and HMGB1 (in B16-OVA cells)	Treated B16-OVA cells efficiently engulfed by monocytes to drive the cross-priming of tumor-specific CD8+ lymphocytes	Prophylactic vaccination model using 8-MOP-PDT-treated B16-OVA, YUMMER and MC38 cells in immunocompetent C57BL/6 mice	82
		Activated alloreactive T cells in vitro, peripheral blood mononuclear cells from GVHD patients	Apoptosis (PtdSer exposure)	Surface exposure of CRT, release of HMGB1. No release of ATP	Treated cells efficiently engulfed by macrophages and DC differentiated from monocytes. Absence of phenotypic maturation of DCs (CD80low, CD40low) and absence of IL-12, IL-6 and IFN-γ secretion by DCs. No ability to stimulate and polarize naive T cells by DCs	N/D	84
Rose Bengal acetate	Cytoskeleton; mitochondria; Golgi apparatus; ER	HeLa human cervical carcinoma	Apoptosis and autophagy (staining for PtdSer exposure and MDC)	Surface exposure of CRT, HSP70 and HSP90 on apoptotic cells; exposure of HSP70 and HSP90 on autophagic cells; release of HSP70, HSP90, ATP (only by apoptotic cells) and HMGB1 (by secondary necrotic cells)	N/D	N/D	65

ATP, adenosine triphosphate; CD, cluster of differentiation; CRT, calreticulin; DC, dendritic cell; ER, endoplasmic reticulum; GVHD, graft versus host disease; HLA, human leukocyte antigen; HMGB1, high-mobility group protein box 1; HSP, heat shock protein; ICAM-1, intercellular adhesion molecule 1; IFN, interferon; IL, interleukin; MDC, monodansylcadaverine; MHC, major histocompatibility complex; N/D, not detected; NK, natural killer; NO, nitric oxide; PARP, poly ADP ribose polymerase; PBMC, platelet-containing peripheral blood mononuclear cell; PDT, photodynamic therapy; PI, propidium iodide; PLAUR, urokinase plasminogen activator surface receptor; PS, photosensitizer; PtdSer, phosphatidylserine; TI, transimmunization; WB, western blot analysis.

The other promising non-porphyrin PS is benzophenazine OR141,80 which also localizes specifically in the ER. OR141 induces cell death mainly through the mammalian target of rapamycin signaling pathway and by inhibition of proteasomal deubiquitinases, resulting in ER stress. CRT exposure and release of HSP90, annexin A1, HMGB1 and ATP on treatment with OR141-PDT has been reported for several cell lines.52 71 In a therapeutic vaccination model, dying cells induced marrow-derived dendritic cell maturation (CD80^high, CD86^high, MHC II^high) in vitro as well as in vivo and led to a delay of tumor growth and an increase in survival of syngeneic mice with SCC7 murine head and neck carcinoma52 or Ab1 mesothelioma81 (table 1). Importantly, the DC-based vaccine proved to be even more effective than vaccination with the PDT-treated carcinoma cells themselves.71

ICD can also be induced by PDT based on 8-methoxypsoralen (8-MOP). This PS has a different mechanism of photo-induced toxicity: it does not require oxygen but intercalates into DNA and forms cross-links with one or two DNA strands on UVA irradiation. 8-MOP is applied in extracorporeal photochemotherapy for cutaneous T-cell lymphoma; white blood cells in peripheral blood are exposed to 8-MOP-UVA and then infused into the patient vasculature. 8-MOP-UVA treatment of murine melanoma cells was shown to result in exposure of CRT and emission of ATP, HMGB1 and type I IFN82 (table 1). In addition, a prophylactic vaccination model proved that ICD can be induced by 8-MOP-UVA treatment in several melanoma cell lines.82 In another study, dying YUMM1.7 murine melanoma cells or MC38 murine colon adenocarcinoma cells pretreated with 8-MOP-UVA were co-incubated with platelet-containing peripheral blood mononuclear cells from tumor-bearing mice. Intravenous reinfusion of the cell mixture into the tumor-bearing mice (multiply repeated procedure) induced a significant delay in tumor growth.83 These data indicate that the selective antitumor effects of extracorporeal photochemotherapy are based on the induction of ICD and suggest that extracorporeal photochemotherapy of cutaneous T cell lymphomas is a potential therapeutic approach. The opposite results were obtained for 8-MOP-UVA-treated peripheral blood mononuclear cells from patients with graft versus host disease and alloreactive T cells.84 Despite pronounced expression of several DAMPs by dying cells, including CRT and HMGB1, and their engulfment by APC, these dying cells did not stimulate DC maturation. These data may be explained by the stage of cell death. It is conceivable that the cell death stage at which dying cells were co-cultured with DC was not immunogenic enough to induce activation/maturation of DCs. In this regard it has been shown that only cells in the early death stage are immunogenic.24

Pronounced production of DAMPs has been reported after treatment with the non-porphyrin PSs listed above, and after application of RBA, a fluorescein derivative.65 The authors showed that the pattern of DAMPs differs between cells dying by apoptosis and those undergoing autophagy: apoptotic cells exposed CRT while autophagic cells did not, and they were not able to release ATP. Yet, the immunogenicity of cell death induced by RBA-ICD must be demonstrated in vivo.85 86

ICD induction by porphyrin photosensitizers of the first generation and second generation

Several porphyrin-derived PSs have shown the ability to induce ICD in PDT-treated cells (figure 2). As early as 2004, it was reported that treatment with the first-generation porphyrin PS, photofrin, led to the exposure of a number of HSPs on the surface of cancer cells and promoted their engulfment by DCs, followed by DC maturation manifested in IL-12 production.73 Of interest, intratumorally injected DCs homed to regional and distant lymph nodes and activated both spontaneous (NK cells) and specific (CD8⁺ T cells) cytotoxicity toward tumor cells. This initial work on photofrin was further extended by others.60 74 It has been shown that photofrin-based PDT of Lewis lung carcinoma cells induced release of HSPs, and surface exposure of CRT in vitro and in vivo within 1 hour after PDT, as well as an increase of HMGB1 in plasma.60 These data indicate that photofrin is a potent inducer of ICD.

One of the promising modes of PDT is based on exogenous aminolevulinic acid (ALA), a low-molecular-weight precursor of protoporphyrin IX. This compound does not accumulate in sufficient amounts in cells with normal metabolism, but in cancer cells its concentration rises significantly mainly due to lowered activity of ferrochelatase, which converts protoporphyrin IX into heme. ALA-PDT induced ICD in two vaccination mouse models. First, ALA-PDT-treated murine SCC cells injected into SKH-1 mice provided complete protection in the tumor prophylactic vaccination model75 (table 2). Second, vaccination of mice with DCs prestimulated by ALA-PDT-treated SSC cells was also shown to be effective against rechallenge with cancer cells. This prophylactic vaccination efficacy is in line with the production of DAMPs, including surface exposure of CRT by ALA-PDT-treated cancer cells of various origin,75 87 as well as HSP70,70 75 88 and release of ATP70 and HMGB175 (table 2). An increase in IFN-I transcription was reported for murine melanoma cells treated with 5-methylaminolevulinic acid (Me-ALA), a derivative of ALA with similar biological properties.87 The expression of IFN-α/IFN-β correlated with the doses of Me-ALA and was specific for PDT-treated cells but not for cells treated with doxorubicin, a bona fide chemotherapeutic ICD inducer. It has been proposed that IFN-I acts in an autocrine loop to induce the apoptosis of treated cells, as well as in a paracrine mode stimulating DC migration (table 2).87

Table 2.

ICD induction by porphyrin photosensitizers (PSs) of the first generation and second generation (tetrapyrrole PS)

PS	Subcellular localization of PS	Cell line	Markers of cell death and cell death types	DAMPs expression	Immunogenicity of cancer cells in vitro	Immunogenicity of cancer cells in vivo	References
Photofrin	Mitochondria; cellular membranes	CT26 murine colon carcinoma	Apoptosis and necrosis (DNA fragmentation analysis by TUNEL in situ)	Surface exposure of HSP27, HSP34, HSP60, HSP72/73, HSP90, GRP78; no surface exposure of HSP70 or GRP94	Stimulation of IL-12 production by DCs	Inoculation of DCs into PDT-treated CT26 tumors growing in BALB/c mice stimulated cytotoxic activity in lymph node and spleen. Spleen lymphocytes produced TNF	73
		SCCVII murine squamous cell carcinoma (SCC)	Apoptosis (flow cytometry: caspase three active form)	Surface exposure of HSP70, HSP60, GRP94; release of HSP70; no surface exposure of GRP78	HSP70 and GRP94 exposure on the macrophages surface, production of TNF-α and NF-κB	PDT-treated SCCVII tumors growing in C3H/HeN mice: surface exposure of HSP70 on leukocytes, and HSP60 and GRP94 on tumor-associated neutrophils and macrophages	74
		LLC murine lung carcinoma (Lewis lung carcinoma)	N/D	Surface exposure of CRT (in vitro and in vivo); ↑ HMGB1 levels in serum	↑ levels of intracellular HMGB1 in macrophages co-incubated with PDT-treated LLC cells	PDT-treated LLC tumors growing in syngeneic C57BL/6 mice: exposure of CRT on the surface of tumor-associated macrophages	60
Protoporphyrin IX (PpIX) induced by exogenic 5-aminolevulinic acid	Mitochondria	GL261 murine glioma	Apoptosis (PtdSer exposure)	Surface exposure of HSP70; release of ATP	N/D	N/D	70
		U87 human glioblastoma, U251 human glioblastoma, astrocytoma	Apoptosis (DNA fragmentation analysis by TUNEL staining)	Surface exposure of HSP70	Phenotypic maturation of DCs (CD40high, CD80high, CD83high, CD86high)	N/D	88
		PECA murine SCC	Apoptosis and necrosis (PtdSer exposure with a vital dye staining)	N/D	Phenotypic maturation of DCs (CD80high, CD86high, MHC IIhigh), production of IFN-γ and IL-12	Prophylactic PDT-DC-based vaccination model in SKH-1 mice	136
		PECA murine SSC	Apoptosis (DNA fragmentation analysis by TUNEL staining)	Surface exposure of CRT and HSP70; release of HMGB1 and HSP70 in PECA cells (in vitro and in vivo)	Phenotypic maturation of DCs (CD80high, CD86high, MHC IIhigh), production of IFN-γ and IL-12	Prophylactic vaccination model using ALA-PDT-treated SCC cells in SKH-1 mice	75
PpIX induced by endogenic 5-methylaminolevulinic acid	ER	B16-OVA murine melanoma	Apoptosis (PtdSer exposure)	Surface exposure of CRT; enhanced expression of INF-1	Phenotypic maturation of DCs (CD86high, MHC IIhigh)	N/D	87
Meso-tetrahydroxyphenyl chlorin	ER	EMT6 murine mammary carcinoma	N/D	Surface exposure and release of HSP70 (in vitro and in vivo)	N/D	N/D	72
		SCCVII murine SCC	N/D	N/D	N/D	PDT-treated SCCVII tumors growing in C3H/HeN mice: rapid ↑ blood neutrophils	137
Glycoconjugated chlorin (G-chlorin)	Mitochondria	CT26 murine colon carcinoma	Apoptosis (flow cytometry: caspase three active form)	Surface exposure of CRT and release of HMGB1 (in vitro and in vivo)	N/D	Prophylactic vaccination model using G-chlorin-PDT-treated CT26 cells in BALB/c mice	61
Photodithazine	ER and Golgi apparatus	GL261 murine glioma, MCA205 murine sarcoma	Apoptosis in GL261 (inhibitors analysis, PtdSer exposure with a vital dye staining)	Surface exposure of CRT; release of ATP and HMGB1	Phenotypic maturation of DCs (CD40high, CD86high, MHC IIhigh), production of IL-6	Prophylactic vaccination model using photodithazine-PDT-treated MCA205 cells in immunocompetent C57BL/6J mice	46
Photosens	Lysosomes	GL261 murine glioma, MCA205 murine sarcoma	Apoptosis and ferroptosis in GL261 (inhibitors analysis, PtdSer exposure with a vital dye staining)	Surface exposure of CRT; release of ATP and HMGB1	Phenotypic maturation of DCs (CD40high, CD86high, MHC IIhigh), production of IL-6	Prophylactic vaccination model using Photosens-PDT-treated MCA205 cells in immunocompetent C57BL/6J mice	46
2-[1-Hexyloxyethyl]-2-devinyl pyropheophorbide (HPPH)	Mitochondria	Colon26-HA murine colorectal carcinoma, 4T1 murine mammary carcinoma	Apoptosis (caspase-3 activity and TUNEL assay138)	N/D	N/D	Tumor-bearing BALB/c mice that remained tumor free after the treatment were challenged with Colo26 or 4T1 cells. (A tumor-controlling PDT regimen was combined with an immune-enhancing PDT regimen)	89
Sodium Porfimer (Photofrin)
Hydrophilic bacteriochlorin (F2BOH)	N/D	CT26 murine colorectal carcinoma	Apoptosis and necrosis (PtdSer exposure with a vital dye staining)	N/D	N/D	PDT-F2BOH cured CT26 tumor-bearing BALB/c mice rejected tumor re-inoculation 1 year after the treatment	139

CD, cluster of differentiation; CRT, calreticulin; DC, dendritic cell; ER, endoplasmic reticulum; GRP, glucose-regulated protein; HMGB1, high-mobility group protein box 1; HSP, heat shock protein; IFN, interferon; IL, interleukin; MHC, major histocompatibility complex; N/D, not detected; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B; PDT, photodynamic therapy; PtdSer, phosphatidylserine; TNF, tumor necrosis factor; TUNEL, terminal deoxynucleotidyl transferase-mediated nick end labeling analysis.

Recently, ICD induction was shown for PDT based on several second-generation porphyrin PSs. In this regard, PDT based on glucose-linked tetra(fluorophenyl)chlorin (G-chlorin) induced an ICD in CT26 murine colon carcinoma cells characterized by surface exposure of CRT and release of HMGB161 (table 2). Vaccination of immunocompetent mice with CT26 cells pretreated with G-chlorin-PDT protected them against a subsequent challenge with live CT26 cells. The role of DAMPs production by these dead tumor cells was demonstrated by the absence of a vaccination effect when tumor cells in which the CRT or HMGB1 gene was knocked-down were used in the vaccination experiment.

Another study compared ICD induction by PDT based on chlorin e6 derivative photodithazine with that based on the phthalocyanine dye photosens.46 The authors showed that both PSs induce ICD associated with DAMPs emission in murine MCA205 fibrosarcoma and GL261 glioma cells.46 However, the intensity and timeline of CRT exposure and release of ATP and HMGB1 by cancer cells depended on both the cell line and the PS. Photosens-based PDT led to a more active engulfment of dead/dying cancer cells by BMDCs and, at least for GL261 glioma cells, a larger increase in the expression of CD40 and CD86 co-stimulatory molecules on the surface of BMDCs. However, both PSs were comparably efficient in a mouse tumor prophylactic vaccination model. The most intriguing aspect of the ICD-inducing capability of photosens is that it has strong vesicular localization. The negative charge and hydrophilic properties of photosens hamper its escape from endosomes and lysosomes. In contrast to most of the PSs studied, the primary target of photosens-PDT is not the ER. Importantly, the cell death induced by photosens combines features of apoptosis and ferroptosis, as it was blocked by specific inhibitors of apoptosis (zVAD-fmk) and ferroptosis (ferrostatin-1 and deferoxamine)46 (table 2). This suggests that certain PSs can induce ICD with mixed cell-death phenotypes. This can be particularly interesting when cancer cells develop resistance to a specific type of cell death. In such cases, triggering several cell-death types makes it possible to circumvent cell death resistance and may increase the efficiency of cell death induction in cancer cells.

A new combined treatment strategy has been proposed based on two PSs and on the ability of PDT to directly kill tumor cells and to initiate antitumor immunity.89 The pheophorbide-derivative 2-[1-hexyloxyethyl]−2-devinyl pyropheophorbide-α (HPPH) and photofrin were applied for two-step PDT: an immune-enhancing low-dose PDT treatment was followed by a tumor-controlling high-dose PDT treatment (table 2). This combined PDT regimen led to higher numbers of activated tumor-specific CD8⁺ T cells in the tumor-draining lymph nodes, and this coincided with reduced metastatic ability of the tumor (ie, murine Colon26-HA and mammary 4T1 carcinomas). It was also associated with enhanced long-term control of tumor growth and resistance of the cured mice to tumor rechallenge. This work indicates that combined PDT may provide an effective adjuvant for therapies that fail to stimulate the host antitumor immune response.

There are several intriguing findings supporting a rationale for combination treatments of PDT based on radachlorin (also known as bremachlorin) and immunotherapy. When the lysates of TC-1 cells carrying human papillomavirus 16 E7 were induced by radachlorin-based PDT in combination with the immuno-adjuvant CpG-oligodeoxynucleotide (ODN), tumor growth after both prophylactic and therapeutic vaccination doses in vivo were significantly suppresssed.90 Interestingly, PDT-cell lysates induced release of DAMPs, including HSP70, and in combination with ODN injection, IFN-γ production and cytotoxic T lymphocytes responses (CD8⁺ T cells) were stronger than when ODN or PDT was used alone.90 Similar effects were shown for a combination of radachlorin-based PDT of TC-1 cells and adenoviral delivery of interleukin-12 (AdmIL-12) in a mouse tumor model.91 In that work, combined treatment significantly increased the production of IFN-γ and TNF-α and the expansion of the CTL subset mediated by CD8⁺ T cells, resulting in complete regression of tumors of 9 mm in mice.91 In another study in the mouse model of therapeutic vaccination, RMA cells (aggressive T-cell lymphoma cell line induced by Rauscher murine leukemia virus) were treated with bremachlorin-based PDT in combination with synthetic long peptides containing epitopes from tumor antigens. This procedure resulted in efficient induction a significant CD8⁺ T-cell response against the tumor.92 All these results indicate that contemporary antitumor treatments should be based on a combination of several antitumor strategies where activation of the immune system is crucial.

Third-generation PSs: enhancing immunogenicity by use of nanoconstructs

The concept of targeted PDT has been actively pursued in recent years. This approach is closely linked to the design of the third-generation PSs, namely, liposomal forms of PSs, PSs in combination with nanocarriers and PSs conjugated with sugar molecules, monoclonal antibodies or peptides (figure 2). The advantages of targeted PDT compared with the insufficient selectivity of first-generation and second-generation PSs are improvement of PS pharmacokinetics, significant reduction in the required dose of the PSs and decrease of side effects.38 One of the requirements for an ‘ideal’ PS is that it has absorption in the near infra-red spectrum (NIR) (600–1000 nm). In this range, light is slightly scattered by most tissues and poorly absorbed by endogenous chromophores such as melanin, hemoglobin and cytochromes. Consequently, far-red and NIR light can penetrate well in human tissue and can be selectively absorbed by photosensitizing agents such as porphyrins, chlorins, phthalocyanines and naphthalocyanines.29 In recent decades, researchers in the field of PDT have decided to combine the use of NIR PSs with their delivery to the target organs by antibodies or nanocarriers. This approach has increased the efficiency of PDT and broadened the boundaries for the treatment of tumors at different sites.93 The strategy of using NIR PSs conjugated with the guiding antibody has been called near-infrared photoimmunotherapy (NIR-PIT).94 Notably, NIR-PIT has been shown to induce ICD. For instance, Ogawa et al95 revealed that NIR-PIT with Tra-IR700 induces translocation of DAMPs (CRT, Hsp70 and Hsp90) to the cell surface and is associated with the rapid release of ATP and HMGB1, followed by maturation of DCs (table 3).

Table 3.

ICD induction by targeted and nanoparticle-incorporated photosensitizers (PS) of the third generation

PS	Subcellular localization of PS	Cell line	Markers of cell death and cell death types	DAMPs expression	Immunogenicity of cancer cell in vitro	Immunogenicity of cancer cells in vivo	References
Cetuximab-IR700	Bind to HER1-overexpressed on the plasma membrane of cancer cells	A431 human epidermoid carcinoma	N/D	Surface exposure of HSP70, HSP90 and CRT; release of ATP and HMGB1	Phenotypic maturation of DCs (CD80high, CD86high, MHC IIhigh), production of IL-12	NIR-PIT-treated A431cells growing in homozygotic athymic nude mice: CD86+ population of DCs, ↑ CD11c, CD205 and MHC II positive cells	95
Chlorin-based nanoscale metal-organic framework	N/D	CT26 murine colorectal carcinoma	Apoptosis and necrosis (flow cytometry: PtdSer and PI)	Surface exposure of CRT	N/D	N/D	96
		MC38 murine colon adenocarcinoma	Apoptosis and necrosis (flow cytometry: PtdSer exposure and PI)	Surface exposure of CRT	N/D	PDT+IDO inhibitor-treated MC38 tumors growing in C57BL/6 mice: ↑ tumor-infiltrating neutrophils, B cells, CD45+ leukocytes, CD4+ T cells, NK cells and CD8+ T cells	140
Core-shell nanoscale coordination polymer conjugated with pyropheophorbide lipid (NCP@pyrolipid)	N/D	CT26 murine colorectal carcinoma, MC38 murine colon adenocarcinoma	Apoptosis and necrosis (flow cytometry: PtdSer exposure and PI)	Surface exposure of CRT on CT26 cells	N/D	Prophylactic vaccination model using PDT-treated CT26 cells in BALB/c mice: ↑ TNF-α, IL-6 and IFN-γ in serum. PDT and PD-L1 blockade-treated MC38 tumors growing in C57BL/6 mice: ↑ antigen-specific IFN- γ-producing T cells in splenocytes, ↑ infiltrating CD8+ T cells in primary and distant tumors, ↑ infiltrating CD45+ and CD4+ T cells in distant tumors	97
Zn-pyrophosphate nanoparticles loaded with the PS pyrolipid (ZnP@pyro)	N/D	4T1, TUBO murine mammary carcinoma	Apoptosis and/or necrosis (flow cytometry, confocal imaging: PtdSer exposure and PI)	Surface exposure of CRT (in vitro and in vivo)	N/D	PDT-treated 4T1 tumors growing in orthotopic mice: ↑ TNF-α, IL-6 and IFN-γ in serum. PDT+anti-PD-L1-treated TUBO tumors growing in syngeneic mice: ↑ CD8+, CD45+ and CD4+ T cells and B cells	98
Graphene oxide conjugated with the HK peptide, coated with a PS HPPH (GO(HPPH)-PEG-HK)	N/D	4T1 murine mammary carcinoma	Necrosis (flow cytometry: PtdSer exposure and 7-AAD)	N/D	Phenotypic maturation of DCs (CD80high, CD86 high)	PDT-treated 4T1 cells growing in BALB/c mice: ↑ CD40 and CD70, maturation markers of DCs, ↑ IFN-γ in serum, ↑ CD8+ T cells. Prophylactic vaccination model using PDT-treated 4T1 necrotic cells in BALB/c mice	99
Core-shell gold nanocage coated with manganese dioxide (AuNC@MnO2)	N/D	4T1 murine mammary carcinoma	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT (in vitro and in vivo), release of ATP and HMGB1	Phenotypic maturation of DCs (CD83 high, CD86 high), production of IL-12	PDT-treated 4T1 cells growing in BALB/c mice: ↑ CD11c+CD86+ and CD11c+CD83+ DCs in tumors, ↑ NK cells in tumors and CD8+ and CD4+ in both tumors and TDLNs, ↓ intratumoral Tregs	106
Hybrid protein oxygen nanocarrier with chlorin e6 encapsulated (C@HPOC).	N/D	4T1 murine mammary carcinoma	N/D	Surface exposure of CRT (in vitro and in vivo); release of HMGB1 and ATP	Phenotypic maturation of DCs (surface upregulation of CD86 and MHC II)	PDT-treated 4T1 tumor-bearing BALB/c mice: tumor infiltration of NK cells, CD8+ and CD4+ T cells, ↑ DC maturation, ↑ activated NK cells, CD8+ and CD4+ T cells both in tumors and TDLNs	109
Hyaluronidase-responsive size-reducible biomimetic nanoparticles, coated with RBC membrane and loaded with PS pheophorbide A, ROS-responsive paclitaxel dimer prodrug (PXTK) and anti-PD-L1 peptide dPPA (pPP-mCAuNCs@HA)	HA bound to CD44 overexpressed on 4T1 cells	4T1 murine mammary carcinoma	N/D	Surface exposure of CRT; release of HMGB1	N/D	Treatment of 4T1 tumor-bearing BALB/c mice with pPP-mCAuNCs@HA+laser: intratumoral infiltration of CD8+ and CD4+T cells, proliferation of CD8+ T cells, ↑ NK cells and CD4+ T cells in blood circulation, ↑ TNF-α and IL-12 in serum	141
Redox-activated porphyrin-based liposome remote-loaded with indoximod (IND@RAL)	RAL co-localized in lysosomes, then PS was released and translocated to the whole cytoplasm, including mitochondria	4T1 murine mammary carcinoma	Apoptosis (flow cytometry: positive staining to annexin V-FITC)	Surface exposure of CRT (in vitro and in vivo); release of ATP and HMGB1	N/D	IND@RAL-PDT treated 4T1 cells growing in BALB/c mice: infiltration of cytotoxic T lymphocyte, ↑ CD3+CD8+ leukocytes but not CD3+CD4+ T cells in the tumor tissues, ↑ cytotoxic T cell and ↓ Tregs in the peripheral blood	142
Nanosystem consists of ER-targeting pardaxin (FAL) peptides modified, indocyanine green (ICG)-conjugated hollow gold nanospheres (FAL-ICG-HAuNS), together with an oxygen-delivering hemoglobin liposome (FAL-Hb lipo)	ER	CT26 murine colon carcinoma, B16 murine melanoma	Apoptosis in CT26 cells (WB: caspase-3 cleavage and CHOP expression)	Surface exposure of CRT in CT26 and B16 cells	N/D	PDT+PTT-treated CT26 cells growing in BALB/c mice: DC maturation in lymph nodes, ↑ MHC I and MHC II on tumor surface, ↑ CD8+ T cells, ↓ Tregs in spleen tissue, ↑ TNF-α and IFN-γ levels in blood. PDT+PTT-treated B16 cells growing in BALB/c mice: ↑ CD11c+/CD80+/CD86+ DCs in lymph nodes, ↑ CD4+ T cells and CD8+ T cells in spleen tissue, infiltration of CD3+ T cells, ↑ CD8+ T cells and ↓ Tregs in tumor tissue, ↑ IL6, TNF-α, IFN-γ and ↓ IL-10 levels in tumor	45
The covering macrophage membrane with shape changeable carriers (chlorin e6/BR-FFVLK-PEG) co-incorporated with paclitaxel (PTX) and indoximod (IND) in the core (I-P@NPs@M)	N/D	4T1 murine mammary carcinoma	Apoptosis (flow cytometry: positive staining to annexin V-FITC)	Surface exposure of CRT and HMGB1; release of ATP and HMGB1 (in vitro); expression of HMGB1 and CRT (in vivo)	N/D	Therapeutic and prophylactic vaccination model using PDT-treated 4T1 cells in BALB/c mice: ↑ CTLs and ↓ Tregs in tumors, ↑ circulating CD4+, CTLs and NKs, ↑ IL-2, IL-12, IFN-γ and TNF-α levels, ↓ IL-10 level, ↑ mature DCs in spleen (CD11c+ CD80high, CD11c+ CD83high or CD11c+ CD86high)	143
pH-responsive nanovesicles self-assembled from block copolymer polyethylene glycol b-cationic polypeptide co-encapsulated with a PS HPPH and indoximod (pRNVs/HPPH/IND)	Endosome/lysosome then drug released in cytoplasm and ER	B16F10 murine melanoma	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT	N/D	pRNVs/HPPH/IND-PDT treated B16F10 cells growing in C57BL/6 mice: ↑ IL-6 and TNF-α levels in blood, reduction both primary and distant tumor growth by infiltration of CD8+ T cells	144
Phase-transition nanoparticles loaded with perfluoropentane, indocyanine green and oxaliplatin (OI_NPs)	N/D	ID8 murine ovarian surface epithelium	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT; release of ATP; translocation and release of HMGB1	N/D	Prophylactic vaccination model using OI_NPs-PSD treated ID8 cells in C57BL/6 mice; T lymphocytes from the spleens of OI_NPs+PSDT immunized mice were capable of lysing ID8 cells in an E/ T-dependent pattern	145
Janus nanobullets integrating chlorin e6 loaded, disulfide-bridged mesoporous organosilica bodies with magnetic heads, cloaked with breast cancer cell membrane (CM@M-MON@Ce6)	N/D	MCF-7 human ductal carcinoma, 4T1 murine mammary carcinoma	N/D	Surface exposure of CRT; release of HMGB1 (in MCF-7 cells); release of HMGB1 (for 4T1 cells in vivo)	Phenotypic maturation of DCs (CD11c, CD80, CD86)	CM@M-MON@Ce6-magnetic hyperthermia-PDT treated 4T1 tumors growing in BALB/c mice: ↑ IL-6, IFN-γ and TNF-α levels. The ratio of CD8+ T cells to CD4+ T cells reduced the percentage of Tregs in the tumor	146
The metal–organic framework-based nanoparticles self-assembled from H2TCPP and zirconium ions with hypoxia inducible factor signaling inhibitor (ACF) and immunogenic adjuvant (CpG) loading, and hyaluronic acid coating on the surface (PCN-ACF-CpG@HA)	Hyaluronic acid bind to CD44-overexpressed on the plasma membrane of H22 cancer cells	H22 murine hepatocellular carcinoma	N/D	N/D	Phenotypic maturation of DCs (CD83high, CD86 high, CD317 high, MHC II high), production of IL-12p70, IFN-γ and TNF-α	PDT-treatment of H22 tumor-bearing BALB/c mice: DC maturation in TDLNs, ↑ IL-12p70, IFN-γ and TNF-α at the tumor site, infiltrating CD8+ and CD4+ T cells at the tumor site	147
Nanoparticles for targeting mitochondria via co-assembling doxorubicin with triphenylphosphonium-tailored IR780 derivative (T780) along with bovine serum albumin as biomimetic corona (BSA@T780/DOX NPs)	Mitochondria	4T1 murine mammary carcinoma	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT; release of HSP70	Proliferation of T cells co-incubated with photo-chemotherapeutic-treated 4T1 cells	Treatment of 4T1 tumor-bearing BALB/c mice with BSA@T780/DOX NPs: ↑ CD4+ and CD8+ T cells, ↑ ratios of CD8+ T cells or CD4+ T cells to Tregs in splenic lymphocytes and distant tumors; ↑ CD8+CD44+CD122+ memory T cell ratio in splenic lymphocytes, ↓ Tregs in splenic lymphocytes and distant tumors, ↑ IL-6 and IFN-γ levels in serum	148
Serum albumin-coated boehmite (B; aluminum hydroxide oxide) organic-inorganic scaffold, loaded with chlorin e6, and a honeybee venom melittin (MLT) peptide (Ce6/MLT@SAB)	Cytoplasm (for Ce6/MLT@SA), endosomal/lysosomal distribution for Ce6	4T1 murine mammary carcinoma	Apoptosis/secondary necrosis (flow cytometry: PI)	Surface exposure of CRT; release of ATP	Maturation of BMDCs (upregulation of CD80 and CD86)	Photo-treatment of 4T1 tumor-bearing BALB/c mice with anti-PD-1+Ce6/MLT@SAB: ↑ CD4+ and CD8+ T cells. No effect on the number of myeloid-derived suppressor cells	149
Polydopamine nanoparticles coated with an upconversion layer of NaGdF4:Yb/Er shell and surface-loaded chlorin e6 (PDA@UCNP-PEG/Ce6)	N/D	4T1 murine mammary carcinoma	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT	N/D	Treatment of 4T1 tumor-bearing BALB/c mice with PDT/PTT: DCs maturation in TDNLs (CD80high, CD86 high); ↑ CD4+ and CD8+ T cells in the spleen; ↑ IL-6, MCP-1, IFN-γ, TNF-α and IL-12p70 levels in serum, but ↓ IL-10 level. Treating 4T1 tumor-bearing BALB/c mice with PDA@UCNP-PEG/Ce6 nanoprobes+PD-1 blockade antibody: ↑ CD8+ T cells and IFN-γ level (expressed by CD4 and CD8 cells) but ↓ Tregs in the spleen and lymph node; ↑ macrophages, B cells and effector memory T cells in the spleen; ↑ IL-6 and TNF-α levels in serum	150
Iridium photocatalyst	Mitochondria	A549 human lung carcinoma	Apoptosis (fluorescent microscopy: PtdSer exposure)	Surface exposure of CRT; release of HMGB1	N/D	N/D	151
Liposomes encapsulated in protoporphyrin IX conjugated with NLG919 (IDO inhibitor) (PpIX-NLG@Lipo)	N/D	4T1 murine mammary carcinoma	Apoptosis/secondary necrosis (flow cytometry: PtdSer exposure and PI)	Surface exposure of CRT; release of ATP	N/D	Treatment of 4T1 tumor-bearing BALB/c mice with PpIX-NLG@Lipo-PDT: ↑ CD8+ T cells infiltration into distant tumor sites	152
Smart nano-enabled platform, in which IR780 and tirapazamine were coloaded in poly(ε-caprolactone)-poly(ethylene glycol) (PEG-PCL) (PEG-PCL-IR780-TPZ NPs)	N/D	4T1 murine mammary carcinoma	N/D	Surface exposure of CRT (in vitro and in vivo); release of ATP and HMGB1	BMDCs maturation (surface upregulation of CD83 and CD86), production of IL-12.	Treatment of BALB/c mice with PEG-PCL-IR780-TPZ NPs+laser-treatment of the 4T1 tumors: ↑ CD11c+CD86+ DCs; infiltration of activated CD8+ T cells into tumors	153
Light-inducible nanocargo (LINC) (reduction-responsive heterodimer of PS pheophorbide A (PPa)+NLG919 (IDO-1 inhibitor+oxaliplatin). LINC were preirradiated with 671 nm laser to cleave the PEG corona (LINCL)	N/D	4T1 murine mammary carcinoma	Apoptosis (for 4T1 treated with LINC+laser) (identified by H&E and TUNEL staining)	Surface exposure of CRT and release of ATP and HMGB1 (for both LINC +laser and LINCL +laser). Surface exposure of CRT and release of HMGB1 of LINCL/ NC@PPt (NLG919-free)+laser treatment in vivo.	BMDCs maturation (surface upregulation of CD80 and CD86).	Treatment of BALB/c mice bearing 4T1 tumors with LINCL +laser irradiation: DC maturation in TDLNs, intratumoral secretion of IFN-γ and TNF-α, intratumoral infiltration of CD8+ T cells, ↓ intratumoral Treg, ↑ CD8+ T cells to Tregs ratio in the tumor mass, ↑ frequency of TEM but ↓ frequency of TCM, ↑ IFN-γ and TNF-α levels in serum. Treatment of 4T1 tumors growing in BALB/c mice with LINCL+laser inhibited tumor growth after rechallenge with 4T1 cells	154
Cu-5,10,15,20-tetrabenzoatoporphyrin (Cu-TBP) nanoscale metal-organic framework (nMOF)	N/D	B16F10 murine melanoma	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT	N/D	Treatment of B16F10 tumor-bearing C57BL/6 mice with Cu-TBP+light irradiation+a-PD-L1: ↑ antigen-specific IFN-γ-producing T cells in spleen; infiltration of CD4+ and CD8+T cells, ↑ CD45+ cells, macrophages and DCs in primary and distant tumors, infiltration of neutrophils to primary tumors. Cu-TBP+light irradiation+a-PD-L1-cured B16F10 tumor-bearing C57BL/6 mice showed resistance to subsequent challenge with B16F10 cells	155
cRGD target liposome with the thymidine conjugate (2 Cl compound) (cRGD-lipo 2 Cl)	cRGD binds to αvβ3 integrin receptor, overexpressed on the plasma membrane of cancer cells	MCF-7 human breast cancer, H22 murine hepatocellular carcinoma	N/D	Increased CRT+ cell population; release of ATP and HMGB1	N/D	Prophylactic vaccination model using cRGD-lipo 2Cl+UVA activation treated H22 cells in BALB/c mice	144
Core-shell gold nanocage coated with manganese dioxide and hyaluronic acid (AMH)	Hyaluronic acid binds to CD44-overexpressed on the plasma membrane of CT26. WT cancer cells	CT26 WT murine colon carcinoma	Apoptosis (flow cytometry: PtdSer exposure)	Surface exposure of CRT; release of ATP	Phenotypic maturation of DCs (CD83high, CD86 high, MHC II high)	N/D	107
PEG-s-s-1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(amino(polyethylene glycol) 2000)nanoparticles loaded with TCPP-TER (4,4',4'',4'''-(porphyrin-5,10,15,20-tetrayl)tetrakis(N-(2-((4methylphenyl)sulfonamido)ethyl)benzamide) (Ds-sP/TCPP-TER NPs)	ER	4T1 murine mammary carcinoma	N/D	Surface exposure of CRT; release of HMGB1 (↓ expression of HMGB1 after release); expression of HMGB1 and CRT (in vivo)	Maturation of BMDCs (surface upregulation of CD80 and CD86), production of TNF-α and IL-12p40	PDT-treated 4T1 tumor-bearing mice: ↑ CD8+ T cells in the primary and distant tumors, ↑ IL-12P40, TNF-α and INF-γ levels in peripheral blood serum	156

BMDC, bone marrow-derived dendritic cell; CD, cluster of differentiation; CHOP, C/EBP-homologous protein-10; CRT, calreticulin; DC, dendritic cell; ER, endoplasmic reticulum; HAuNS, hollow gold nanospheres; HMGB1, high-mobility group protein box 1; HSP, heat shock protein; ICD, immunogenic cell death; IFN, interferon; IL, interleukin; MHC, major histocompatibility complex; N/D, not detected; NK, natural killer; PDT, photodynamic therapy; PI, propidium iodide; PS, photosensitizer; PtdSer, phosphatidylserine; TDLNs, tumor draining lymph nodes; TNF, tumor necrosis factor; T_regs, regulatory T cells; TUNEL, terminal deoxynucleotidyl transferase-mediated nick end labeling analysis; WB, western blot analysis.

Increased absorption in the NIR spectrum range can also be achieved by using a nanoscale metal-organic framework (NMOF). For instance, Lu et al demonstrated that the application of porphyrin-based NMOF, DBC-UiO, leads to an increase in the extinction coefficient96 (table 3). This indicates that these compounds may be useful for medical procedures based on light absorption in NIR tissue transparency window. In addition to changes in the photophysical properties of such structures, the increase in PDT efficiency was associated with CRT surface exposure and ICD induction.

Another approach for ICD induction in PDT is the use of checkpoint inhibitors such as antibodies inhibiting programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1). He et al reported the use of nanoscale coordination polymer core-shell nanoparticles carrying oxaliplatin in the core and a PS pyropheophorbide-lipid conjugate (pyrolipid) in the shell (NCP@pyrolipid) in combination with anti-PD-L1 therapy. This induced CRT surface exposure, effective antitumor vaccination and an abscopal effect.97 A similar effect was described in another study in which Zn-pyrophosphate (ZnP) nanoparticles were loaded with pyrolipid (ZnP@pyro) in combination with anti-PD-L1 therapy98 (table 3).

Besides combining PDT with checkpoint inhibitors, several studies describe the use of peptide-conjugated PSs. For example, graphene oxide coated with a photosensitizer (HPPH) and conjugated with a HK peptide (tumor integrin αvβ6-targeting peptide) was able to destroy the primary tumor site and residual tumor cells, prevent the occurrence of remote metastases, activate the host’s antitumor immunity and suppress tumor relapse by stimulating immunological memory in mice.99 All these results indicate that third-generation PSs hold promise for enhancing the immunogenicity of dying cancer cell therapy and for combining PDT with checkpoints inhibitors. Nevertheless, most studies have been done on mouse models, and this strategy requires validation in clinical studies. It should be noted that nanocarriers can also be used to increase the immunogenicity of dying cancer cells,39 and this is an exciting area for future research.

Challenges and future trends in PDT-induced ICD

The efficiency of PDT induction of ICD can be compromised by hypoxia, which often occurs in the tumor microenvironment. The hypoxic process is mediated by the rapid proliferation of cancer cells and leads to a significant imbalance between oxygen supply and demand and pronounced metabolic alterations.100 The development of oxygen deficiency in the tumor microenvironment is also facilitated by pathophysiological changes such as deformation of tumor blood vessels due to an imbalance between pro-angiogenic and anti-angiogenic signaling, physical compression and disruption of the functioning of the lymphatic system.101 102 Since PDT treatment relies on an oxygen supply for induction of cytotoxic ROS production, hypoxia significantly diminishes PDT efficiency for solid tumors. Therefore, it is important to develop strategies to overcome this hypoxia-related limitation of PDT. The effectiveness of PDT in triggering ICD can be improved by the introduction of agents that increase the oxygen concentration in the tumor microenvironment, a strategy named oxygen-boosted PDT. One approach is to develop versatile oxygen carriers or generators, such as nanoparticles based on perfluocarbon, which is used in artificial blood in clinical applications.103 Thanks to its high oxygen capacity, perfluocarbon provides a long ¹O₂ lifetime, which results in long-lasting photodynamic effects.104 However, in that study, though the therapeutic efficacy of PDT was established in experiments on tumor-bearing mice, the immunogenicity of tumor cell death was not examined. Therefore, additional studies are needed to understand whether this strategy can induce ICD.

Other strategies are linked to the creation of nanoparticles based on manganese dioxide (MnO₂). MnO₂ degradation in the acidic and H₂O₂-rich tumor microenvironment generates sufficient oxygen and increases ROS production, which in turn increases PDT efficacy. In addition, the Mn(ΙΙ) ion reduced from Mn(ΙV) in response to increased acidic H₂O₂ provides an opportunity to carry out selective MRI in vivo.105 Of interest, core-shell gold nanocages encapsulated in MnO₂ (AuNC@MnO₂) altered the hypoxic and immunosuppressive tumor microenvironment and demonstrated reliable PDT and ICD effects. Oxygen-boosted PDT based on such nanoparticles is characterized by the emission of DAMPs such as CRT, ATP and HMGB1, followed by DC maturation and subsequent effector cell activation, including CD8⁺ and CD4⁺ T cells and NK cells. It has been shown that this provoked an antitumor immune response and effectively inhibited tumor growth and recurrence in two different tumor models (mice with CT26 colorectal and 4T1 mammary carcinomas).106 107

Oxygen supply to the tumor microenvironment can also be augmented by using hemoglobin-based nanostructures. Directed delivery of hemoglobin to tumor cells via hybrid nanostructures ensures gradual O₂ release in a hypoxic environment and significantly enhances ROS production during PDT.108 109 It was also shown that chlorin e6-based PDT can trigger an ICD characterized by DAMPs release (CRT, HMGB1, ATP), DC maturation and activation of CD4⁺ and CD8⁺ T lymphocytes and NK cells in vivo. Activation of the immune system by hemoglobin-based nanostructures was shown to eradicate primary tumors and effectively suppress distant tumor growth and lung metastasis in a murine metastatic triple-negative breast cancer model.109 Thus, overcoming hypoxia in the tumor microenvironment is essential for increasing PDT efficiency. Development of hybrid systems based on nanoconstructs that can relieve hypoxia and include an immunological component is the most promising strategy, and we may see a new milestone for PDT in anticancer treatment in the near future.

In conclusion, the insights from the last several years increasingly support the idea that PDT is a powerful strategy for inducing ICD in experimental cancer therapy. However, most studies have focused on mouse models, but it is necessary to validate this strategy in clinical settings. Moreover, further insights into the interplay between PDT and oxygen-boosted therapy may provide new ground for the development of novel cancer immunotherapy. PDT and ICD represent a challenging research area with many possible promising future applications in the treatment of cancer.

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