Structural Characterization of Streptococcus pneumoniae Serotype 9A Capsule Polysaccharide Reveals Role of Glycosyl 6-O-Acetyltransferase wcjE in Serotype 9V Capsule Biosynthesis and Immunogenicity

Supplemental Data

Files in this Data Supplement:

• Supplemental Table (.pdf, 91 KB) - Assignment of signals in 9VdO 1H-13C HMQC
• Supplemental Figure 1 (.pdf, 154 KB) - 1H NMR spectra of native and de-O-acetylated (dO) 9V and 9A PS
• Supplemental Figure 2 (.pdf, 194 KB) - 1H-13C HMQC spectra of 9VdO
• Supplemental Figure 3 (.pdf, 219 KB) - NMR spectra of JC01 (9V) and JC02 (9A) capsule PS
• Supplemental Figure 4 (.pdf, 255 KB) - Dose-dependent binding of serotyping antibodies to 9V (black square, solid line), 9A (gray triangle, solid line), 9VdO (black square, dotted line), and 9AdO (gray triangle, dotted line) PS-coated plates
• Supplemental Figure 5 (.pdf, 87 KB) - Dose-dependent binding of human serum antibodies to 9A and 9V capsule PS