Allosteric Regulation of the Carbohydrate-binding Ability of a Novel Conger Eel Galectin by d-Mannoside

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure S1 (.pdf, 188 KB) - RT-PCR analysis of RNA from the peritoneal cells of conger eel using common primers for congerins (A) and RNAs from various tissues using specific primers for individual RNAs of Con-P, Con I and ConII (B).
• Supplemental Table S1 (.pdf, 90 KB) - Ka value of Con I, Con II, and Con-P for each PA sugar.
• Supplemental Table S2 (.pdf, 90 KB) - Amount of the conjugated protein on Con-P-conjugated and Con II-conjugated columns used for FAC analysis.
• Supplemental Figure S2 (.pdf, 376 KB) - Purification of Con II-tagged Con-P by affinity chromatography on an HCl-treated sepharose 4B column.
• Supplemental Figure S3 (.pdf, 5.9 MB) - Nano LC-MS/MS analysis of Con II-tagged Con-P.
• Supplemental Figure S4 (.pdf, 105 KB) - Stability of recombinant Con-P.
• Supplemental Figure S5 (.pdf, 150 KB) - Distribution of apparent molecular weight of Con-P (A) and Con II-tagged Con-P (B).
• Supplemental Figure S6 (.pdf, 137 KB) - Elution profiles of FAC analysis of Con-P (A) and Con II-tagged Con-P (B) for PA-sugar No. 3, NA3 (type1), with and without mannose.
• Supplemental Figure S7 (.pdf, 155 KB) - Determination of the column binding capacity (Bt) for Con-P conjugated (Con-P) and Con II conjugated (Con II) columns by Lineweaver-Burk plots (A) and Woolfe-Hofstee-type plots (B).
• Supplemental Figure S8 (.pdf, 115 KB) - Fluorescence spectra of Con-P with mannose titration.
• Supplemental Figure S9 (.pdf, 111 KB) - Fluorescence spectra of Con-P (A) and the titration curve of yeast mannan (B).
• Supplementary data (.doc, 40 KB) - SUPPLEMENTARY DATA SUPPLEMENTAL METHODS and LEGENDS FOR SUPPLEMENTARY FIGURES