Isolation and Characterization of Reticuline N-Methyltransferase Involved in Biosynthesis of the Aporphine Alkaloid Magnoflorine in Opium Poppy

Supplemental Data

• Supplemental Data File 1 (.pdf, 675 KB) - NMR spectra
• Supplemental Figure 1 (.pdf, 270 KB) - Cobalt-affinity purification of recombinant His6-tagged RNMT (NMT3) and NMT4.
• Supplemental Figure 2 (.pdf, 114 KB) - Steady state enzyme kinetics, and pH and temperature optima for recombinant RNMT using (S)-reticuline and (R)-reticuline as substrates.
• Supplemental Figure 3 (.pdf, 619 KB) - Reverse transcription (RT)-PCR and transcript-metabolite correlation analysis of RNMT-silenced (pTRV2-RNMT) and control (pTRV2) plants.
• Supplemental Figure 4 (.pdf, 808 KB) - Extracted ion chromatograms (EICs) for alkaloids identified in root alkaloid extracts from RNMT-silenced (pTRV2-RNMT) and control (pTRV2) plants.
• Supplemental Table 1 (.pdf, 72 KB) - Summary of NMR data for tembetarine.
• Supplemental Table 2 (.pdf, 92 KB) - Chromatographic and mass spectral data for authentic benzylisoquinoline alkaloid standards.