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. 1986 Oct;114(2):347–361. doi: 10.1093/genetics/114.2.347

Frequency and Directionality of Gene Conversion Events Involving the CYC7-H3 Mutation in SACCHAROMYCES CEREVISIAE

Patricia J Pukkila 1,2,3, Michael D Stephens 1,2,3, David M Binninger 1,2,3, Beverly Errede 1,2,3
PMCID: PMC1202944  PMID: 3021569

Abstract

The CYC7-H3 mutation is a 5-kb deletion that causes overproduction of iso-2 cytochrome c. Unlike most mutations in yeast, the CYC7-H3 mutation is preferentially lost when it is involved in a gene conversion event. We have shown that cloned copies of CYC7-H3 DNA that are inserted into the yeast genome are associated with a high frequency of recombination and aberrant segregation events. Since parity in conversion frequency was observed when the extensive insertion/deletion heterozygosity at this locus was eliminated, we conclude that the CYC7-H3 sequences are inherently capable of acting as donors or recipients in gene conversion events, although they are unlikely to act as donors when they are located opposite a large heterology. DNA sequence comparisons revealed similarities between the CYC7-H3 junction region and the 2-µm circle DNA region that is involved in site-specific recombination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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