Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1988 Jul;87(3):551–554. doi: 10.1104/pp.87.3.551

Simultaneous Transport of CO2 and HCO3− by the Cyanobacterium Synechococcus UTEX 625 1

George S Espie 1, Anthony G Miller 1, Douglas G Birch 1, David T Canvin 1
PMCID: PMC1054795  PMID: 16666182

Abstract

A mass spectrometer was used to simultaneously follow the time course of photosynthetic O2 evolution and CO2 depletion of the medium by cells of the cyanobacterium Synechococcus leopoliensis UTEX 625. Analysis of the data indicated that both CO2 and HCO3− were simultaneously and continuously transported by the cells as a source of substrate for photosynthesis. Initiation of HCO3− transport by Na+ addition had no effect on ongoing CO2 transport. This result is interpreted to indicate that the CO2 and HCO3− transport systems are separate and distinctly different transport systems. Measurement of CO2-dependent photosynthesis indicated that CO2 uptake involved active transport and that diffusion played only a minor role in CO2 acquisition in cyanobacteria.

Full text

PDF
551

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger M. R., Andrews T. J. Photosynthesis and Inorganic Carbon Usage by the Marine Cyanobacterium, Synechococcus sp. Plant Physiol. 1982 Aug;70(2):517–523. doi: 10.1104/pp.70.2.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Espie G. S., Canvin D. T. Evidence for Na-Independent HCO(3) Uptake by the Cyanobacterium Synechococcus leopoliensis. Plant Physiol. 1987 May;84(1):125–130. doi: 10.1104/pp.84.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOCH G., KOK B. A mass spectrometer inlet system for sampling gases dissolved in liquid phases. Arch Biochem Biophys. 1963 Apr;101:160–170. doi: 10.1016/0003-9861(63)90546-0. [DOI] [PubMed] [Google Scholar]
  4. Miller A. G., Colman B. Active transport and accumulation of bicarbonate by a unicellular cyanobacterium. J Bacteriol. 1980 Sep;143(3):1253–1259. doi: 10.1128/jb.143.3.1253-1259.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miller A. G., Colman B. Evidence for HCO(3) Transport by the Blue-Green Alga (Cyanobacterium) Coccochloris peniocystis. Plant Physiol. 1980 Feb;65(2):397–402. doi: 10.1104/pp.65.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Miller A. G., Espie G. S., Canvin D. T. Active Transport of CO(2) by the Cyanobacterium Synechococcus UTEX 625 : Measurement by Mass Spectrometry. Plant Physiol. 1988 Mar;86(3):677–683. doi: 10.1104/pp.86.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller A. G., Turpin D. H., Canvin D. T. Growth and Photosynthesis of the Cyanobacterium Synechococcus leopoliensis in HCO(3)-Limited Chemostats. Plant Physiol. 1984 Aug;75(4):1064–1070. doi: 10.1104/pp.75.4.1064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller A. G., Turpin D. H., Canvin D. T. Na+ requirement for growth, photosynthesis, and pH regulation in the alkalotolerant cyanobacterium Synechococcus leopoliensis. J Bacteriol. 1984 Jul;159(1):100–106. doi: 10.1128/jb.159.1.100-106.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Volokita M., Zenvirth D., Kaplan A., Reinhold L. Nature of the Inorganic Carbon Species Actively Taken Up by the Cyanobacterium Anabaena variabilis. Plant Physiol. 1984 Nov;76(3):599–602. doi: 10.1104/pp.76.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES