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. 1986 Nov;82(3):840–845. doi: 10.1104/pp.82.3.840

Cytoplasmic pH Regulation in Acer pseudoplatanus Cells

I. A 31P NMR Description of Acid-Load Effects

Jean Guern 1,2, Yves Mathieu 1,2, Michel Pean 1,2,1, Corinne Pasquier 1,2, Jean-Claude Beloeil 1,2, Jean-Yves Lallemand 1,2
PMCID: PMC1056216  PMID: 16665119

Abstract

Modifications of cytoplasmic pH in Acer pseudoplatanus L. cells cultivated in suspension have been induced by acid-loads and studied by using 31P nuclear magnetic resonance spectroscopy. The initial drop of cytoplasmic pH, observed in the first minutes of exposure to weak lipophilic acids, was followed by a slow recovery to reach a plateau phase with a pH value lower than the initial one. Conversely, removal of the acid led to a sharp increase of cytoplasmic pH with in most cases an overshoot toward more alkaline values than the initial one and a subsequent decrease to more acidic values. This shows that A. pseudoplatanus cells powerfully regulate their cytoplasmic pH both on the acid side of their normal pH, during the acid-load, and on the alkaline side, after removal of acid. Similar results were obtained with different types of acid-loads, i.e. treatments with propionic or benzoic acid or bubbling with CO2-enriched air. This indicates that the occurrence of pH regulation does not depend upon the method used to acid-load the cells. The time courses of cytoplasmic pH observed for A. pseudoplatanus and also Catharanthus roseus cells are similar to those recorded for animal cells but different from those described for other plant materials for which no recovery phase was observed. This can be explained by different balances between the initial rate of proton influx brought in by the acids, and the capacity of proton consumption by the regulatory mechanisms. The existence of the recovery phase offers a unique possibility to study the regulation of the cytoplasmic pH of plant cells, as it has been done in animal systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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