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. 1990 Jun;93(2):584–587. doi: 10.1104/pp.93.2.584

Induction of Pyrophosphate:Fructose 6-Phosphate 1-Phosphotransferase by Anoxia in Rice Seedlings 1

Emmanuel Mertens 1,2, Yvan Larondelle 1,2, Henri-Géry Hers 1,2
PMCID: PMC1062554  PMID: 16667507

Abstract

Rice (Oryza sativa) seeds were imbibed for 3 days and the seedlings were further incubated for 8 days in the presence of either air or nitrogen. In aerobiosis, the specific activity of pyrophosphate:fructose 6-phosphate 1-phosphotransferase and that of the ATP-dependent phosphofructokinase increased about fourfold. In anaerobiosis, the specific activity of ATP-dependent phosphofructokinase remained stable, whereas that of pyrophosphate:fructose 6-phosphate 1-phosphotransferase increased as much as in the presence of oxygen and there was also a fourfold increase in the concentration of fructose 2,6-bisphosphate, a potent stimulator of that enzyme. These data suggest a preferential involvement of pyrophosphate:fructose 6-phosphate 1-phosphotransferase rather than of ATP-dependent phosphofructokinase in glycolysis during anaerobiosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APP A. A., MEISS A. N. Effect of aeration on rice alcohol dehydrogenase. Arch Biochem Biophys. 1958 Sep;77(1):181–190. doi: 10.1016/0003-9861(58)90054-7. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Carnal N. W., Black C. C. Phosphofructokinase activities in photosynthetic organisms : the occurrence of pyrophosphate-dependent 6-phosphofructokinase in plants and algae. Plant Physiol. 1983 Jan;71(1):150–155. doi: 10.1104/pp.71.1.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carnal N. W., Black C. C. Pyrophosphate-dependent 6-phosphofructokinase, a new glycolytic enzyme in pineapple leaves. Biochem Biophys Res Commun. 1979 Jan 15;86(1):20–26. doi: 10.1016/0006-291x(79)90376-0. [DOI] [PubMed] [Google Scholar]
  5. Hers H. G., Hue L. Gluconeogenesis and related aspects of glycolysis. Annu Rev Biochem. 1983;52:617–653. doi: 10.1146/annurev.bi.52.070183.003153. [DOI] [PubMed] [Google Scholar]
  6. Huber S. C., Akazawa T. A novel sucrose synthase pathway for sucrose degradation in cultured sycamore cells. Plant Physiol. 1986 Aug;81(4):1008–1013. doi: 10.1104/pp.81.4.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kruger N. J., Beevers H. Synthesis and degradation of fructose 2,6-bisphosphate in endosperm of castor bean seedlings. Plant Physiol. 1985 Feb;77(2):358–364. doi: 10.1104/pp.77.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Larondelle Y., Mertens E., Van Schaftingen E., Hers H. G. Fructose 2,6-bisphosphate hydrolyzing enzymes in higher plants. Plant Physiol. 1989 Jul;90(3):827–834. doi: 10.1104/pp.90.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Larondelle Y., Mertens E., Van Schaftingen E., Hers H. G. Purification and properties of spinach leaf phosphofructokinase 2/fructose 2,6-bisphosphatase. Eur J Biochem. 1986 Dec 1;161(2):351–357. doi: 10.1111/j.1432-1033.1986.tb10454.x. [DOI] [PubMed] [Google Scholar]
  10. Mertens E., Van Schaftingen E., Müller M. Presence of a fructose-2,6-bisphosphate-insensitive pyrophosphate: fructose-6-phosphate phosphotransferase in the anaerobic protozoa Tritrichomonas foetus, Trichomonas vaginalis and Isotricha prostoma. Mol Biochem Parasitol. 1989 Dec;37(2):183–190. doi: 10.1016/0166-6851(89)90150-3. [DOI] [PubMed] [Google Scholar]
  11. Mocquot B., Prat C., Mouches C., Pradet A. Effect of anoxia on energy charge and protein synthesis in rice embryo. Plant Physiol. 1981 Sep;68(3):636–640. doi: 10.1104/pp.68.3.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sabularse D. C., Anderson R. L. D-Fructose 2,6-bisphosphate: a naturally occurring activator for inorganic pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase in plants. Biochem Biophys Res Commun. 1981 Dec 15;103(3):848–855. doi: 10.1016/0006-291x(81)90888-3. [DOI] [PubMed] [Google Scholar]
  13. Van Schaftingen E. Fructose 2,6-bisphosphate. Adv Enzymol Relat Areas Mol Biol. 1987;59:315–395. doi: 10.1002/9780470123058.ch7. [DOI] [PubMed] [Google Scholar]
  14. Van Schaftingen E., Hers H. G. Formation of fructose 2,6-bisphosphate from fructose 1,6-bisphosphate by intramolecular cyclisation followed by alkaline hydrolysis. Eur J Biochem. 1981 Jul;117(2):319–323. doi: 10.1111/j.1432-1033.1981.tb06339.x. [DOI] [PubMed] [Google Scholar]
  15. Van Schaftingen E., Lederer B., Bartrons R., Hers H. G. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. Eur J Biochem. 1982 Dec;129(1):191–195. doi: 10.1111/j.1432-1033.1982.tb07039.x. [DOI] [PubMed] [Google Scholar]
  16. Wood H. G. Some reactions in which inorganic pyrophosphate replaces ATP and serves as a source of energy. Fed Proc. 1977 Aug;36(9):2197–2206. [PubMed] [Google Scholar]
  17. Xu D. P., Sung S. J., Loboda T., Kormanik P. P., Black C. C. Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway. Plant Physiol. 1989 Jun;90(2):635–642. doi: 10.1104/pp.90.2.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ap Rees T., Green J. H., Wilson P. M. Pyrophosphate:fructose 6-phosphate 1-phosphotransferase and glycolysis in non-photosynthetic tissues of higher plants. Biochem J. 1985 Apr 1;227(1):299–304. doi: 10.1042/bj2270299. [DOI] [PMC free article] [PubMed] [Google Scholar]

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