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. 1985 Jan;77(1):74–78. doi: 10.1104/pp.77.1.74

Acclimation to Low Temperature by Microsomal Membranes from Tomato Cell Cultures

Frances M Dupont 1,1, J Brian Mudd 1
PMCID: PMC1064459  PMID: 16664031

Abstract

Sealed vesicles were prepared from microsomal membranes from cell suspension cultures of tomato (Lycopersicon esculentum Mill cv VF36). ATP-dependent proton transport activity by the vesicles was measured as quenching of fluorescence of acridine orange. Measurements of proton transport were correlated with the activity of a nitrate-inhibitable ATPase. The initial rate of proton influx into the vesicles was strongly temperature dependent with a Q10 of 2 and a maximum rate near 35°C. The data suggest that passive permeability did not increase at chilling temperatures but did increase rapidly with temperatures above 30°C. A comparison was made between membranes from cell cultures grown at 28°C and 9°C. The temperature optimum for proton transport broadened and shifted to a lower temperature range in membranes from cells maintained at 9°C.

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Selected References

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