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. 1983 Oct;73(2):398–406. doi: 10.1104/pp.73.2.398

Purification and Separation of Plant Gibberellins from Their Precursors and Glucosyl Conjugates 1

Masaji Koshioka 1, Kiyotashi Takeno 1, Frederick D Beall 1, Richard P Pharis 1,2
PMCID: PMC1066473  PMID: 16663228

Abstract

A procedure using two small preparative columns (in sequence) of C18 reverse phase Bondapak B material with methanolic extracts of plant tissue (Pisum sativum L., Malus domestica Borkh., Pimpinella anisum L.) yields two fractions: (i) gibberellin (GA) precursors, and (ii) free GA/GA methyl esters (GA-Me)/GA glucosyl conjugates. The discrete separation of (iii) free GA/GA-Me from (iv) GA glucosyl conjugates is then accomplished by a combination of differential solvent solubility and SiO2 partition chromatography. All fractions are almost pigment free, and appreciable dry weight purification was accomplished for the GA precursor and free GA/GA-Me fractions. Solvent volumes can be kept low, no buffer salts are introduced, and each fraction (i, iii, iv) can be subjected directly to preparative or analytical reverse phase C18 high performance liquid chromatography without recourse to solvent partitioning, and often without further purification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coombe B. G. GA32: A Polar Gibberellin with High Biological Potency. Science. 1971 May 21;172(3985):856–857. doi: 10.1126/science.172.3985.856. [DOI] [PubMed] [Google Scholar]
  2. Jones M. G., Metzger J. D., Zeevaart J. A. Fractionation of gibberellins in plant extracts by reverse phase high performance liquid chromatography. Plant Physiol. 1980 Feb;65(2):218–221. doi: 10.1104/pp.65.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Metzger J. D., Zeevaart J. A. Identification of six endogenous gibberellins in spinach shoots. Plant Physiol. 1980 Apr;65(4):623–626. doi: 10.1104/pp.65.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Sembdner G., Schneider G., Weiland J., Schreiber K. Ueber ein gebundenes Gibberellin aus Phaseolus coccineus L. Experientia. 1964 Feb 15;20(2):89–90. doi: 10.1007/BF02151258. [DOI] [PubMed] [Google Scholar]
  5. Zeevaart J. A. Effects of photoperiod on growth rate and endogenous gibberellins in the long-day rosette plant spinach. Plant Physiol. 1971 Jun;47(6):821–827. doi: 10.1104/pp.47.6.821. [DOI] [PMC free article] [PubMed] [Google Scholar]

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