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. 1984 Jun;75(2):502–504. doi: 10.1104/pp.75.2.502

A Rapid Reverse Phase Evaporation Method for the Reconstitution of Uncharged Thylakoid Membrane Lipids That Resist Hydration 1

Sallie G Sprague 1, L Andrew Staehelin 1
PMCID: PMC1066939  PMID: 16663653

Abstract

Comparison of several lipid reconstitution methods showed that they were not equally efficient at transferring the predominant thylakoid lipid, monogalactosyldiglyceride (MG), to the aqueous phase. We report a reverse phase evaporation method that employs Freon 11 as a lipid solvent and is capable of successfully hydrating MG in spinach (Spinacia oleracea L.) at room temperature within minutes. Using this method it is possible to force an equal weight mixture of MG and digalactosyldiglyceride into small bilayer vesicles without the formation of inverted micellar `lipidic particles' in the membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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