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. 1992 Apr 15;283(Pt 2):531–535. doi: 10.1042/bj2830531

Comparative study of asparagine-linked glycans of plasma T-kininogen in normal rats and during acute inflammation.

T Baussant 1, C Alonso 1, J M Wieruszeski 1, G Strecker 1, J Montreuil 1, F Alhenc-Gelas 1, J C Michalski 1
PMCID: PMC1131068  PMID: 1575697

Abstract

Rat T-kininogen has been separated into two molecular variants by affinity chromatography on concanavalin A (ConA): a ConA-reactive (ConA+) and a ConA-non-reactive (ConA-) fraction, from which carbohydrate chains were quantitatively released by hydrazinolysis. On the basis of high-resolution 400 MHz 1H-n.m.r. spectroscopy of the re-N-acetylated hydrazinolysates, the carbohydrate structures of the two ConA molecular variants of rat T-kininogen were established. The ConA-non-reactive species contains a single type of carbohydrate chain with the following structure: [formula: see text] The ConA-reactive fraction contains the same structure and the following additional one: [formula: see text] The relative abundance of the two molecular forms is profoundly affected during inflammation (ratio ConA+/ConA-: 44% in normal and 95% in inflamed T-kininogen), but no structural modification of the carbohydrate chains was observed.

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Selected References

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