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. 1992 Dec 1;288(Pt 2):553–564. doi: 10.1042/bj2880553

Expression of a cardiac Ca(2+)-release channel isoform in mammalian brain.

F A Lai 1, M Dent 1, C Wickenden 1, L Xu 1, G Kumari 1, M Misra 1, H B Lee 1, M Sar 1, G Meissner 1
PMCID: PMC1132046  PMID: 1334409

Abstract

Mammalian brain possesses ryanodine-sensitive Ca2+ channels, which in muscle cells mediate rapid Ca2+ release from intracellular stores during excitation-contraction coupling. Analysis of bovine brain ryanodine receptor (RyR) channels suggests specific expression of the cardiac-muscle RyR isoform in mammalian brain. Localization using cardiac-muscle RyR-specific antibodies and antisense RNA revealed that brain RyRs were present in dendrites, cell bodies and terminals of rat forebrain, and highly enriched in the hippocampus. Activity of skeletal-muscle RyR channels is coupled to sarcolemmal voltage sensors, in contrast with cardiac-muscle RyR channels, which are known to be Ca(2+)-induced Ca(2+)-release channels. Thus Ca(2+)-induced Ca2+ release from intracellular stores mediated by brain RyR channels may be a major Ca(2+)-signalling pathway in specific regions of mammalian brain, and hence may play a fundamental role in neuronal Ca2+ homoeostasis.

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