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. 1986 May 15;236(1):273–278. doi: 10.1042/bj2360273

Intralipid administration induces a lipoprotein lipase-like activity in the livers of starved adult rats.

S Vilaró, M Reina, I Ramírez, M Llobera
PMCID: PMC1146816  PMID: 3790076

Abstract

The administration of Intralipid to starved adult rats induces the appearance of lipoprotein lipase (LPL)-like activity in the liver, whereas the so-called hepatic triacylglycerol lipase is unaffected. This LPL-like activity is eluted by 1.5 M-NaCl from heparin-Sepharose columns. This partially purified fraction is inhibited by 1.0 M-NaCl (91%) and by 1.0 mg of protamine sulphate/ml (79%), whereas it is stimulated 69-fold by the presence of 8.0 micrograms of apolipoprotein C-II/ml and inhibited by anti-LPL antibodies. We conclude that Intralipid administration induces the appearance of LPL activity in livers of starved adult rats. Its possible origin is discussed.

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Selected References

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  1. Assmann G., Krauss R. M., Fredrickson D. S., Levy R. I. Characterization, subcellular localization, and partial purification of a heparin-released triglyceride lipase from rat liver. J Biol Chem. 1973 Mar 25;248(6):1992–1999. [PubMed] [Google Scholar]
  2. Belfrage P., Vaughan M. Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J Lipid Res. 1969 May;10(3):341–344. [PubMed] [Google Scholar]
  3. Bensadoun A., Koh T. L. Identification of an adipose tissue-like lipoprotein lipase in perfusates of chicken liver. J Lipid Res. 1977 Nov;18(6):768–773. [PubMed] [Google Scholar]
  4. Chajek T., Stein O., Stein Y. Pre- and post-natal development of lipoprotein lipase and hepatic triglyceride hydrolase activity in rat tissues. Atherosclerosis. 1977 Apr;26(4):549–561. doi: 10.1016/0021-9150(77)90122-8. [DOI] [PubMed] [Google Scholar]
  5. Chung J., Scanu A. M. Isolation, molecular properties, and kinetic characterization of lipoprotein lipase from rat heart. J Biol Chem. 1977 Jun 25;252(12):4202–4209. [PubMed] [Google Scholar]
  6. Cryer A. Tissue lipoprotein lipase activity and its action in lipoprotein metabolism. Int J Biochem. 1981;13(5):525–541. doi: 10.1016/0020-711x(81)90177-4. [DOI] [PubMed] [Google Scholar]
  7. Ehnholm C., Kinnunen P. K., Huttunen J. K. Heterogeneity of salt-resistant lipase from human postheparin plasma. FEBS Lett. 1975 Apr 1;52(2):191–194. doi: 10.1016/0014-5793(75)80804-0. [DOI] [PubMed] [Google Scholar]
  8. Fielding C. J., Fielding P. E. Mechanism of salt-mediated inhibition of lipoprotein lipase. J Lipid Res. 1976 May;17(3):248–256. [PubMed] [Google Scholar]
  9. Garfinkel A. S., Schotz M. C. Separation of molecular species of lipoprotein lipase from adipose tissue. J Lipid Res. 1972 Jan;13(1):63–68. [PubMed] [Google Scholar]
  10. Grinberg D. R., Ramírez I., Vilaró S., Reina M., Llobera M., Herrera E. Starvation enhances lipoprotein lipase activity in the liver of the newborn rat. Biochim Biophys Acta. 1985 Feb 8;833(2):217–222. doi: 10.1016/0005-2760(85)90193-6. [DOI] [PubMed] [Google Scholar]
  11. Groot P. H., Jansen H., Van Tol A. Selective degradation of the high density lipoprotein-2 subfraction by heparin-releasable liver lipase. FEBS Lett. 1981 Jul 6;129(2):269–272. doi: 10.1016/0014-5793(81)80181-0. [DOI] [PubMed] [Google Scholar]
  12. Jahn C. E., Osborne J. C., Jr, Schaefer E. J., Brewer H. B., Jr Activation of the enzymic activity of hepatic lipase by apolipoprotein A-II. Characterization of a major component of high density lipoprotein as the activating plasma component in vitro. Eur J Biochem. 1983 Mar 1;131(1):25–29. doi: 10.1111/j.1432-1033.1983.tb07227.x. [DOI] [PubMed] [Google Scholar]
  13. Jensen G. L., Baly D. L., Brannon P. M., Bensadoun A. Synthesis and secretion of lipolytic enzymes by cultured chicken hepatocytes. J Biol Chem. 1980 Dec 10;255(23):11141–11148. [PubMed] [Google Scholar]
  14. Jensen G. L., Bensadoun A. Purification, stabilization, and characterization of rat hepatic triglyceride lipase. Anal Biochem. 1981 May 15;113(2):246–252. doi: 10.1016/0003-2697(81)90073-7. [DOI] [PubMed] [Google Scholar]
  15. KORN E. D. The kinetics of the inhibition of lipoprotein lipase by polyanions and polycations. J Biol Chem. 1962 Nov;237:3423–3429. [PubMed] [Google Scholar]
  16. Kinnunen P. K., Ehnolm C. Effect of serum and C-apoproteins from very low density lipoproteins on human postheparin plasma hepatic lipase. FEBS Lett. 1976 Jun 15;65(3):354–357. doi: 10.1016/0014-5793(76)80145-7. [DOI] [PubMed] [Google Scholar]
  17. Knobler H., Chajek-Shaul T., Stein O., Etienne J., Stein Y. Modulation of lipoprotein lipase in the intact rat by cholera toxin--an irreversible agonist of cyclic AMP. Biochim Biophys Acta. 1984 Sep 12;795(2):363–371. doi: 10.1016/0005-2760(84)90087-0. [DOI] [PubMed] [Google Scholar]
  18. Kuusi T., Schröder T., Bång B., Lempinen M., Ehnholm C. Selective measurement of triacylglycerol lipase activities in pig post-heparin plasma. Biochim Biophys Acta. 1979 Jun 21;573(3):443–450. doi: 10.1016/0005-2760(79)90219-4. [DOI] [PubMed] [Google Scholar]
  19. LaRosa J. C., Levy R. I., Herbert P., Lux S. E., Fredrickson D. S. A specific apoprotein activator for lipoprotein lipase. Biochem Biophys Res Commun. 1970 Oct 9;41(1):57–62. doi: 10.1016/0006-291x(70)90468-7. [DOI] [PubMed] [Google Scholar]
  20. Landin B., Nilsson A., Twu J. S., Schotz M. C. A role for hepatic lipase in chylomicron and high density lipoprotein phospholipid metabolism. J Lipid Res. 1984 Jun;25(6):559–563. [PubMed] [Google Scholar]
  21. Llobera M., Montes A., Herrera E. Lipoprotein lipase activity in liver of the rat fetus. Biochem Biophys Res Commun. 1979 Nov 14;91(1):272–277. doi: 10.1016/0006-291x(79)90613-2. [DOI] [PubMed] [Google Scholar]
  22. Masuno H., Tsujita T., Nakanishi H., Yoshida A., Fukunishi R., Okuda H. Lipoprotein lipase-like activity in the liver of mice with Sarcoma 180. J Lipid Res. 1984 May;25(5):419–427. [PubMed] [Google Scholar]
  23. Nilsson-Ehle P., Schotz M. C. A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res. 1976 Sep;17(5):536–541. [PubMed] [Google Scholar]
  24. Ogata F., Hirasawa Y. Heparin-released triglyceride lipase from Chang liver cells. Biochem Biophys Res Commun. 1982 May 31;106(2):397–399. doi: 10.1016/0006-291x(82)91123-8. [DOI] [PubMed] [Google Scholar]
  25. Olivecrona T., Chernick S. S., Bengtsson-Olivecrona G., Paterniti J. R., Jr, Brown W. V., Scow R. O. Combined lipase deficiency (cld/cld) in mice. Demonstration that an inactive form of lipoprotein lipase is synthesized. J Biol Chem. 1985 Feb 25;260(4):2552–2557. [PubMed] [Google Scholar]
  26. Paterniti J. R., Jr, Brown W. V., Ginsberg H. N., Artzt K. Combined lipase deficiency (cld): a lethal mutation on chromosome 17 of the mouse. Science. 1983 Jul 8;221(4606):167–169. doi: 10.1126/science.6857276. [DOI] [PubMed] [Google Scholar]
  27. Ramírez I., Llobera M., Herrera E. Circulating triacylglycerols, lipoproteins, and tissue lipoprotein lipase activities in rat mothers and offspring during the perinatal period: effect of postmaturity. Metabolism. 1983 Apr;32(4):333–341. doi: 10.1016/0026-0495(83)90040-9. [DOI] [PubMed] [Google Scholar]
  28. Ramírez J. I., Llobera M., Herrera E. Method for triglyceride measurement in small amounts of plasma. Rev Esp Fisiol. 1983 Sep;39(3):327–331. [PubMed] [Google Scholar]
  29. Twu J. S., Garfinkel A. S., Schotz M. C. Purification and characterization of lipoprotein lipase from human heart. Atherosclerosis. 1976 Jul-Aug;24(1-2):119–128. doi: 10.1016/0021-9150(76)90069-1. [DOI] [PubMed] [Google Scholar]
  30. Twu J. S., Garfinkel A. S., Schotz M. C. Rat heart lipoprotein lipase. Atherosclerosis. 1975 Nov-Dec;22(3):463–472. doi: 10.1016/0021-9150(75)90025-8. [DOI] [PubMed] [Google Scholar]
  31. Vainio P., Virtanen J. A., Kinnunen P. K., Gotto A. M., Jr, Sparrow J. T., Pattus F., Bougis P., Verger R. Action of lipoprotein lipase on mixed triacylglycerol/phosphatidylcholine monolayers. Activation by apolipoprotein C-II. J Biol Chem. 1983 May 10;258(9):5477–5482. [PubMed] [Google Scholar]
  32. Waite M., Sisson P., El-Maghrabi R. A comparison of the lipolytic activities in liver perfusates and liver plasma membranes from rats. Biochim Biophys Acta. 1978 Aug 25;530(2):292–298. doi: 10.1016/0005-2760(78)90014-0. [DOI] [PubMed] [Google Scholar]
  33. Wallinder L., Bengtsson G., Olivecrona T. Guinea pig very low density lipoproteins are a good substrate for lipoprotein lipase. Biochim Biophys Acta. 1979 Dec 18;575(3):458–462. doi: 10.1016/0005-2760(79)90115-2. [DOI] [PubMed] [Google Scholar]
  34. Wallinder L., Peterson J., Olivecrona T., Bengtsson-Olivecrona G. Hepatic and extrahepatic uptake of intravenously injected lipoprotein lipase. Biochim Biophys Acta. 1984 Oct 4;795(3):513–524. doi: 10.1016/0005-2760(84)90181-4. [DOI] [PubMed] [Google Scholar]
  35. ZAK B., COHEN J. Automatic analysis of tissue culture proteins with stable Folin reagents. Clin Chim Acta. 1961 Sep;6:665–670. doi: 10.1016/0009-8981(61)90112-7. [DOI] [PubMed] [Google Scholar]

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