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. 1987 Apr 1;243(1):267–271. doi: 10.1042/bj2430267

Effects of lactation and removal of pups on the rate of triacyglycerol/fatty acid substrate cycling in white adipose tissue of the rat.

P Hansson, E A Newsholme, D H Williamson
PMCID: PMC1147842  PMID: 3300638

Abstract

The rate of the triacylglycerol/fatty acid substrate cycle was measured in vivo in adipose tissue of virgin and lactating rats with pups removed. The rate decreased by 70% in adipose tissue of lactating rats and increased 9-fold on removal of the pups. Similar differences in cycling rate were seen in adipose tissue incubated in vitro in the presence of isoprenaline.

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Selected References

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  1. Agius L., Robinson A. M., Girard J. R., Williamson D. H. Alterations in the rate of lipogenesis in vivo in maternal liver and adipose tissue on premature weaning of lactating rats: a possible regulatory role of prolactin. Biochem J. 1979 Jun 15;180(3):689–692. doi: 10.1042/bj1800689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks B. J., Arch J. R., Newsholme E. A. Effect of some hormones on the rate of the triacylglycerol/fatty-acid substrate cycle in adipose tissue of the mouse in vivo. Biosci Rep. 1983 Mar;3(3):263–267. doi: 10.1007/BF01122458. [DOI] [PubMed] [Google Scholar]
  3. Brooks B., Arch J. R., Newsholme E. A. Effects of hormones on the rate of the triacylglycerol/fatty acid substrate cycle in adipocytes and epididymal fat pads. FEBS Lett. 1982 Sep 20;146(2):327–330. doi: 10.1016/0014-5793(82)80945-9. [DOI] [PubMed] [Google Scholar]
  4. Burnol A. F., Leturque A., Ferré P., Girard J. Glucose metabolism during lactation in the rat: quantitative and regulatory aspects. Am J Physiol. 1983 Oct;245(4):E351–E358. doi: 10.1152/ajpendo.1983.245.4.E351. [DOI] [PubMed] [Google Scholar]
  5. Crabtree B., Newsholme E. A. A quantitative approach to metabolic control. Curr Top Cell Regul. 1985;25:21–76. doi: 10.1016/b978-0-12-152825-6.50006-0. [DOI] [PubMed] [Google Scholar]
  6. Cubero A., Ros M., Lobato M. F., Carcía-Ruiz J. P., Moreno F. J. Coordination of glucose metabolism and NADPH formation in the adipose tissue and mammary gland during the lactation-weaning transition. Enzyme. 1983;30(1):38–47. doi: 10.1159/000469543. [DOI] [PubMed] [Google Scholar]
  7. Eggstein M., Kreutz F. H. Eine neue Bestimmung der Neutralfette im Blutserum und Gewebe. I. Prinzip, Durchführung und Besprechung der Methode. Klin Wochenschr. 1966 Mar 1;44(5):262–267. doi: 10.1007/BF01747716. [DOI] [PubMed] [Google Scholar]
  8. FELL B. F., SMITH K. A., CAMPBELL R. M. Hypertrophic and hyperplastic changes in the alimentary canal of the lactating rat. J Pathol Bacteriol. 1963 Jan;85:179–188. doi: 10.1002/path.1700850117. [DOI] [PubMed] [Google Scholar]
  9. Flatt J. P. Conversion of carbohydrate to fat in adipose tissue: an energy-yielding and, therefore, self-limiting process. J Lipid Res. 1970 Mar;11(2):131–143. [PubMed] [Google Scholar]
  10. Flint D. J., Clegg R. A., Vernon R. G. Prolactin and the regulation of adipose-tissue metabolism during lactation in rats. Mol Cell Endocrinol. 1981 May;22(2):265–275. doi: 10.1016/0303-7207(81)90096-4. [DOI] [PubMed] [Google Scholar]
  11. Flint D. J., Sinnett-Smith P. A., Clegg R. A., Vernon R. G. Role of insulin receptors in the changing metabolism of adipose tissue during pregnancy and lactation in the rat. Biochem J. 1979 Aug 15;182(2):421–427. doi: 10.1042/bj1820421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Halperin M. L., Denton R. M. Regulation of glycolysis and L-glycerol 3-phosphate concentration in rat epididymal adipose tissue in vitro. Role of phosphofructokinase. Biochem J. 1969 Jun;113(1):207–214. doi: 10.1042/bj1130207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hamosh M., Clary T. R., Chernick S. S., Scow R. O. Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochim Biophys Acta. 1970 Sep 8;210(3):473–482. doi: 10.1016/0005-2760(70)90044-5. [DOI] [PubMed] [Google Scholar]
  14. Illingworth P. J., Jung R. T., Howie P. W., Leslie P., Isles T. E. Diminution in energy expenditure during lactation. Br Med J (Clin Res Ed) 1986 Feb 15;292(6518):437–441. doi: 10.1136/bmj.292.6518.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jones R. G., Ilic V., Williamson D. H. Physiological significance of altered insulin metabolism in the conscious rat during lactation. Biochem J. 1984 Jun 1;220(2):455–460. doi: 10.1042/bj2200455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jungas R. L. Fatty acid synthesis in adipose tissue incubated in tritiated water. Biochemistry. 1968 Oct;7(10):3708–3717. doi: 10.1021/bi00850a050. [DOI] [PubMed] [Google Scholar]
  17. Moore B. J., Brasel J. A. One cycle of reproduction consisting of pregnancy, lactation or no lactation, and recovery: effects on fat pad cellularity in ad libitum-fed and food-restricted rats. J Nutr. 1984 Sep;114(9):1560–1565. doi: 10.1093/jn/114.9.1560. [DOI] [PubMed] [Google Scholar]
  18. Naismith D. J., Richardson D. P., Pritchard A. E. The utilization of protein and energy during lactation in the rat, with particular regard to the use of fat accumulated in pregnancy. Br J Nutr. 1982 Sep;48(2):433–441. doi: 10.1079/bjn19820125. [DOI] [PubMed] [Google Scholar]
  19. Newsholme E. A., Crabtree B. Substrate cycles in metabolic regulation and in heat generation. Biochem Soc Symp. 1976;(41):61–109. [PubMed] [Google Scholar]
  20. Oller do Nascimento C. M., Williamson D. H. Evidence for conservation of dietary lipid in the rat during lactation and the immediate period after removal of the litter. Decreased oxidation of oral [1-14C]triolein. Biochem J. 1986 Oct 1;239(1):233–236. doi: 10.1042/bj2390233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Robinson A. M., Girard J. R., Williamson D. H. Evidence for a role of insulin in the regulation of lipogenesis in lactating rat mammary gland. Measurements of lipogenesis in vivo and plasma hormone concentrations in response to starvation and refeeding. Biochem J. 1978 Oct 15;176(1):343–346. doi: 10.1042/bj1760343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SPRAY C. M. A study of some aspects of reproduction by means of chemical analysis. Br J Nutr. 1950;4(4):354–360. doi: 10.1079/bjn19500059. [DOI] [PubMed] [Google Scholar]
  23. Saggerson E. D., Greenbaum A. L. The regulation of triglyceride synthesis and fatty acid synthesis in rat epididymal adipose tissue. Biochem J. 1970 Sep;119(2):193–219. doi: 10.1042/bj1190193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Scanu A. M. Factors affecting lipoprotein metabolism. Adv Lipid Res. 1965;3:63–138. [PubMed] [Google Scholar]
  25. Scow R. O., Chernick S. S., Fleck T. R. Lipoprotein lipase and uptake of triacylglycerol, cholesterol and phosphatidylcholine from chylomicrons by mammary and adipose tissue of lactating rats in vivo. Biochim Biophys Acta. 1977 May 25;487(2):297–306. doi: 10.1016/0005-2760(77)90006-6. [DOI] [PubMed] [Google Scholar]
  26. Smith R. W., Walsh A. Effect of lactation on lipolysis in rat adipose tissue. Lipids. 1976 May;11(5):418–420. doi: 10.1007/BF02532850. [DOI] [PubMed] [Google Scholar]
  27. Steinberg D. Fatty acid mobilization--mechanisms of regulation and metabolic consequences. Biochem Soc Symp. 1963;24:111–143. [PubMed] [Google Scholar]
  28. Steingrimsdottir L., Greenwood M. R., Brasel J. A. Effect of pregnancy, lactation and a high-fat diet on adipose tissue in Osborne-Mendel rats. J Nutr. 1980 Apr;110(4):600–609. doi: 10.1093/jn/110.4.600. [DOI] [PubMed] [Google Scholar]
  29. Trayhurn P., Douglas J. B., McGuckin M. M. Brown adipose tissue thermogenesis is 'suppressed' during lactation in mice. Nature. 1982 Jul 1;298(5869):59–60. doi: 10.1038/298059a0. [DOI] [PubMed] [Google Scholar]
  30. Vernon R. G., Finley E. Lipolysis in rat adipocytes during recovery from lactation. Response to noradrenaline and adenosine. Biochem J. 1986 Feb 15;234(1):229–231. doi: 10.1042/bj2340229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Vernon R. G., Flint D. J. Control of fatty acid synthesis in lactation. Proc Nutr Soc. 1983 Jun;42(2):315–331. doi: 10.1079/pns19830035. [DOI] [PubMed] [Google Scholar]
  32. WOLLENBERGER A., RISTAU O., SCHOFFA G. [A simple technic for extremely rapid freezing of large pieces of tissue]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:399–412. [PubMed] [Google Scholar]
  33. Windmueller H. G., Spaeth A. E. Perfusion in situ with tritium oxide to measure hepatic lipogenesis and lipid secretion. Normal and orotic acid-fed rats. J Biol Chem. 1966 Jun 25;241(12):2891–2899. [PubMed] [Google Scholar]

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