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. 1994 Jan 1;474(1):35–41. doi: 10.1113/jphysiol.1994.sp020000

G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes.

K Nakamura 1, T Nukada 1, T Haga 1, H Sugiyama 1
PMCID: PMC1160293  PMID: 8014896

Abstract

1. Metabotropic glutamate receptor subtype 1 (mGluR1), when expressed in Xenopus oocytes, activates phosphoinositide-specific phospholipase C (PLC) in a G protein-dependent manner. This reaction results in the activation of chloride channels in the oocytes, and can be monitored electrophysiologically. We expressed different G protein alpha-subunits together with mGluR1 in oocytes, and examined the effects of these G protein subunits on the PLC-mediated reaction. 2. The expression of the alpha-subunit of GL2, a bovine version of G11, which is a member of the Gq subgroup, potentiated the mGluR1-evoked reaction, whereas the alpha-subunit of GL1, a bovine G14, which is also a member of the Gq subgroup, strongly suppressed it. The expression of Gs alpha also suppressed this reaction. 3. We then expressed G beta 1 gamma 2-subunits in addition to the G alpha-subunits, and examined the mGluR1-evoked reactions. Both the potentiation and suppression by GL2 alpha and GL1 alpha, respectively, were more pronounced in the presence of the G beta 1 gamma 2-subunits. In contrast, the suppression by Gs alpha was completely reversed by G beta 1 gamma 2. 4. The direct activation of G proteins by the intracellular injection of either fluoride ions or guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) causes similar PLC-mediated reactions. The expression of GL2 alpha, GL1 alpha or Gs alpha caused potentiation, suppression and no change, respectively, on the fluoride- (or GTP gamma S-) evoked reactions.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Camps M., Carozzi A., Schnabel P., Scheer A., Parker P. J., Gierschik P. Isozyme-selective stimulation of phospholipase C-beta 2 by G protein beta gamma-subunits. Nature. 1992 Dec 17;360(6405):684–686. doi: 10.1038/360684a0. [DOI] [PubMed] [Google Scholar]
  2. Dascal N., Ifune C., Hopkins R., Snutch T. P., Lübbert H., Davidson N., Simon M. I., Lester H. A. Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA. Brain Res. 1986 Dec;387(3):201–209. doi: 10.1016/0169-328x(86)90026-4. [DOI] [PubMed] [Google Scholar]
  3. Enjalbert A., Guillon G., Mouillac B., Audinot V., Rasolonjanahary R., Kordon C., Bockaert J. Dual mechanisms of inhibition by dopamine of basal and thyrotropin-releasing hormone-stimulated inositol phosphate production in anterior pituitary cells. Evidence for an inhibition not mediated by voltage-dependent Ca2+ channels. J Biol Chem. 1990 Nov 5;265(31):18816–18822. [PubMed] [Google Scholar]
  4. Gautam N., Baetscher M., Aebersold R., Simon M. I. A G protein gamma subunit shares homology with ras proteins. Science. 1989 May 26;244(4907):971–974. doi: 10.1126/science.2499046. [DOI] [PubMed] [Google Scholar]
  5. Ito I., Hirono C., Yamagishi S., Nomura Y., Kaneko S., Sugiyama H. Roles of protein kinases in neurotransmitter responses in Xenopus oocytes injected with rat brain mRNA. J Cell Physiol. 1988 Jan;134(1):155–160. doi: 10.1002/jcp.1041340120. [DOI] [PubMed] [Google Scholar]
  6. Kaneko S., Kato K., Yamagishi S., Sugiyama H., Nomura Y. GTP-binding proteins Gi and Go transplanted onto Xenopus oocyte by rat brain messenger RNA. Brain Res. 1987 Dec;427(1):11–19. doi: 10.1016/0169-328x(87)90039-8. [DOI] [PubMed] [Google Scholar]
  7. Kato K., Kaneko S., Nomura Y. Phorbol ester inhibition of current responses and simultaneous protein phosphorylation in Xenopus oocyte injected with brain mRNA. J Neurochem. 1988 Mar;50(3):766–773. doi: 10.1111/j.1471-4159.1988.tb02980.x. [DOI] [PubMed] [Google Scholar]
  8. Katz A., Wu D., Simon M. I. Subunits beta gamma of heterotrimeric G protein activate beta 2 isoform of phospholipase C. Nature. 1992 Dec 17;360(6405):686–689. doi: 10.1038/360686a0. [DOI] [PubMed] [Google Scholar]
  9. Lee C. H., Park D., Wu D., Rhee S. G., Simon M. I. Members of the Gq alpha subunit gene family activate phospholipase C beta isozymes. J Biol Chem. 1992 Aug 15;267(23):16044–16047. [PubMed] [Google Scholar]
  10. Linden J., Delahunty T. M. Receptors that inhibit phosphoinositide breakdown. Trends Pharmacol Sci. 1989 Mar;10(3):114–120. doi: 10.1016/0165-6147(89)90209-5. [DOI] [PubMed] [Google Scholar]
  11. Masu M., Tanabe Y., Tsuchida K., Shigemoto R., Nakanishi S. Sequence and expression of a metabotropic glutamate receptor. Nature. 1991 Feb 28;349(6312):760–765. doi: 10.1038/349760a0. [DOI] [PubMed] [Google Scholar]
  12. Minakami R., Hirose E., Yoshioka K., Yoshimura R., Misumi Y., Sakaki Y., Tohyama M., Kiyama H., Sugiyama H. Postnatal development of mRNA specific for a metabotropic glutamate receptor in the rat brain. Neurosci Res. 1992 Oct;15(1-2):58–63. doi: 10.1016/0168-0102(92)90017-7. [DOI] [PubMed] [Google Scholar]
  13. Nakamura F., Ogata K., Shiozaki K., Kameyama K., Ohara K., Haga T., Nukada T. Identification of two novel GTP-binding protein alpha-subunits that lack apparent ADP-ribosylation sites for pertussis toxin. J Biol Chem. 1991 Jul 5;266(19):12676–12681. [PubMed] [Google Scholar]
  14. Nukada T., Tanabe T., Takahashi H., Noda M., Hirose T., Inayama S., Numa S. Primary structure of the alpha-subunit of bovine adenylate cyclase-stimulating G-protein deduced from the cDNA sequence. FEBS Lett. 1986 Jan 20;195(1-2):220–224. doi: 10.1016/0014-5793(86)80164-8. [DOI] [PubMed] [Google Scholar]
  15. Simon M. I., Strathmann M. P., Gautam N. Diversity of G proteins in signal transduction. Science. 1991 May 10;252(5007):802–808. doi: 10.1126/science.1902986. [DOI] [PubMed] [Google Scholar]
  16. Smrcka A. V., Hepler J. R., Brown K. O., Sternweis P. C. Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq. Science. 1991 Feb 15;251(4995):804–807. doi: 10.1126/science.1846707. [DOI] [PubMed] [Google Scholar]
  17. Sugimoto K., Nukada T., Tanabe T., Takahashi H., Noda M., Minamino N., Kangawa K., Matsuo H., Hirose T., Inayama S. Primary structure of the beta-subunit of bovine transducin deduced from the cDNA sequence. FEBS Lett. 1985 Oct 28;191(2):235–240. doi: 10.1016/0014-5793(85)80015-6. [DOI] [PubMed] [Google Scholar]
  18. Sugiyama H., Ito I., Hirono C. A new type of glutamate receptor linked to inositol phospholipid metabolism. Nature. 1987 Feb 5;325(6104):531–533. doi: 10.1038/325531a0. [DOI] [PubMed] [Google Scholar]
  19. Wilkie T. M., Scherle P. A., Strathmann M. P., Slepak V. Z., Simon M. I. Characterization of G-protein alpha subunits in the Gq class: expression in murine tissues and in stromal and hematopoietic cell lines. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10049–10053. doi: 10.1073/pnas.88.22.10049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wu D. Q., Lee C. H., Rhee S. G., Simon M. I. Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. J Biol Chem. 1992 Jan 25;267(3):1811–1817. [PubMed] [Google Scholar]

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