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. 1981 Jan 1;193(1):371–374. doi: 10.1042/bj1930371

Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel electrophoretic analysis.

J M Finger, K H Choo
PMCID: PMC1162610  PMID: 7305933

Abstract

Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  2. Choo K. H., Cotton R. G., Danks D. M. Double-labeling and high precision comparison of complex protein patterns on two-dimensional polyacrylamide gels. Anal Biochem. 1980 Mar 15;103(1):33–38. doi: 10.1016/0003-2697(80)90231-6. [DOI] [PubMed] [Google Scholar]
  3. Choo K. H., Cotton R. G., Danks D. M., Jennings I. G. Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuria. Biochem J. 1979 Aug 1;181(2):285–294. doi: 10.1042/bj1810285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dottavio-Martin D., Ravel J. M. Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride. Anal Biochem. 1978 Jul 1;87(2):562–565. doi: 10.1016/0003-2697(78)90706-6. [DOI] [PubMed] [Google Scholar]
  5. Kumarasamy R., Symons R. H. The tritium labeling of small amounts of protein for analysis by electrophoresis on sodium dodecyl sulfate--polyacrylamide slab gels. Anal Biochem. 1979 Jun;95(2):359–363. doi: 10.1016/0003-2697(79)90739-5. [DOI] [PubMed] [Google Scholar]
  6. McConkey E. H. Double-label autoradiography for comparison of complex protein mixtures after gel electrophoresis. Anal Biochem. 1979 Jul 1;96(1):39–44. doi: 10.1016/0003-2697(79)90551-7. [DOI] [PubMed] [Google Scholar]
  7. Means G. E., Feeney R. E. Reductive alkylation of amino groups in proteins. Biochemistry. 1968 Jun;7(6):2192–2201. doi: 10.1021/bi00846a023. [DOI] [PubMed] [Google Scholar]
  8. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  9. Rice R. H., Means G. E. Radioactive labeling of proteins in vitro. J Biol Chem. 1971 Feb 10;246(3):831–832. [PubMed] [Google Scholar]

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