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. 1981 Apr 1;195(1):171–176. doi: 10.1042/bj1950171

Preparation and characterization of histone H1 from the sperm of the sea-urchin Sphaerechinus granularis.

V Giancotti, S Cosimi, P D Cary, C Crane-Robinson, G Geraci
PMCID: PMC1162868  PMID: 7306043

Abstract

The separation and purification of histone H1 from the sperm of the sea-urchin Sphaerechinus granularis is described. Physical studies were used to compare this histone H1 molecule with H1 histones from other species. C.d. and 270 MHz n.m.r. spectroscopy indicate that, despite significant compositional differences from other sea-urchin sperm H1 histones, their secondary and tertiary structures are very similar. A large difference in helicity was, however, found between S. granularis histone H1 and calf thymus histone H1, and their n.m.r. and fluorescence spectra also differ considerably. It is concluded that secondary structure and tertiary structure have not been conserved in the evolution of the H1 histone family.

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Selected References

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