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. 1973 Oct;135(2):315–325. doi: 10.1042/bj1350315

The occurrence of two types of synthesis of deoxyribonucleic acid during normal growth in Bacillus subtilis

William J Harris 1
PMCID: PMC1165825  PMID: 4202939

Abstract

A study of the relative utilization of thymine and thymidine as precursors for DNA synthesis during normal growth in Bacillus subtilis showed that thymine serves preferentially as a precursor for `repair' synthesis, whereas thymidine is used preferentially for `replicative' synthesis. Further, evidence was obtained which suggests that during normal growth both `replicative' and `repair' DNA syntheses occur simultaneously. `Repair' synthesis is distinguished not only on the basis of its preferential utilization of thymine but also by its selective inhibition by caffeine. `Replicative' synthesis, however, is selectively inhibited by 6-(p-hydroxyphenylazo)-uracil. `Repair' synthesis would seem to be a `pre-fork' phenomenon and its inhibition is highly lethal to the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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