Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Feb;138(2):317–320. doi: 10.1042/bj1380317

Effects of collagen and of aspirin on the concentration of guanosine 3′:5′-cyclic monophosphate in human blood platelets: measurement by a prelabelling technique (Short Communication)

R J Haslam 1, M D McClenaghan 1
PMCID: PMC1166211  PMID: 4362746

Abstract

A method is described for isolating cyclic [3H]GMP from platelets preincubated with [3H]guanine. Collagen increased and aspirin decreased the cyclic [3H]GMP concentration in platelets. The results are consistent with a role for cyclic GMP in the platelet release reaction.

Full text

PDF
319

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball G., Brereton G. G., Fulwood M., Ireland D. M., Yates P. Effet of prostaglandin E1 alone and in combination with theophylline or aspirin on collagen-induced platelet aggregation and on platelet nucleotides including adenosine 3':5'-cyclic monophosphate. Biochem J. 1970 Dec;120(4):709–718. doi: 10.1042/bj1200709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Evans G., Packham M. A., Nishizawa E. E., Mustard J. F., Murphy E. A. The effect of acetylsalicyclic acid on platelet function. J Exp Med. 1968 Nov 1;128(5):877–894. doi: 10.1084/jem.128.5.877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Haslam R. J. Interactions of the pharmacological receptors of blood platelets with adenylate cyclase. Ser Haematol. 1973;6(3):333–350. [PubMed] [Google Scholar]
  4. Haslam R. J., Rosson G. M. Aggregation of human blood platelets by vasopressin. Am J Physiol. 1972 Oct;223(4):958–967. doi: 10.1152/ajplegacy.1972.223.4.958. [DOI] [PubMed] [Google Scholar]
  5. Haslam R. J., Taylor A. Effects of catecholamines on the formation of adenosine 3':5'-cyclic monophosphate in human blood platelets. Biochem J. 1971 Nov;125(1):377–379. doi: 10.1042/bj1250377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ignarro L. J. Neutral protease release from human leukocytes regulated by neurohormones and cyclic nucleotides. Nat New Biol. 1973 Oct 3;245(144):151–154. doi: 10.1038/newbio245151a0. [DOI] [PubMed] [Google Scholar]
  7. KREBS H. A., HEMS R. Some reactions of adenosine and inosine phosphates in animal tissues. Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):172–180. doi: 10.1016/0006-3002(53)90136-x. [DOI] [PubMed] [Google Scholar]
  8. Kaliner M., Orange R. P., Austen K. F. Immunological release of histamine and slow reacting substance of anaphylaxis from human lung. J Exp Med. 1972 Sep 1;136(3):556–567. doi: 10.1084/jem.136.3.556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kuo J. F., De Renzo E. C. A comparison of the effects of lipolytic and antilipolytic agents on adenosine 3',5'-monophosphate levels in adipose cells as determined by prior labeling with adenine-8-14C. J Biol Chem. 1969 May 10;244(9):2252–2260. [PubMed] [Google Scholar]
  10. Mustard J. F., Packham M. A. Factors influencing platelet function: adhesion, release, and aggregation. Pharmacol Rev. 1970 Jun;22(2):97–187. [PubMed] [Google Scholar]
  11. Ramwell P. W., Shaw J. E. Biological significance of the prostaglandins. Recent Prog Horm Res. 1970;26:139–187. doi: 10.1016/b978-0-12-571126-5.50008-x. [DOI] [PubMed] [Google Scholar]
  12. Salzman E. W. Cyclic AMP and platelet function. N Engl J Med. 1972 Feb 17;286(7):358–363. doi: 10.1056/NEJM197202172860708. [DOI] [PubMed] [Google Scholar]
  13. Scales B. A new scintillator for liquid scintillation counting. Int J Appl Radiat Isot. 1967 Jan;18(1):1–6. doi: 10.1016/0020-708x(67)90165-2. [DOI] [PubMed] [Google Scholar]
  14. Shimizu H., Daly J. Formation of cyclic adenosine 3',5'-monophosphate from adenosine in brain slices. Biochim Biophys Acta. 1970 Nov 24;222(2):465–473. doi: 10.1016/0304-4165(70)90137-6. [DOI] [PubMed] [Google Scholar]
  15. Smith J. B., Willis A. L. Aspirin selectively inhibits prostaglandin production in human platelets. Nat New Biol. 1971 Jun 23;231(25):235–237. doi: 10.1038/newbio231235a0. [DOI] [PubMed] [Google Scholar]
  16. Turtle J. R., Kipnis D. M. A new assay for adenosine 3',5'-cyclic monophosphate in tissue. Biochemistry. 1967 Dec;6(12):3970–3976. doi: 10.1021/bi00864a044. [DOI] [PubMed] [Google Scholar]
  17. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]
  18. Weiss H. J., Aledort L. M. Impaired platelet-connective-tissue reaction in man after aspirin ingestion. Lancet. 1967 Sep 2;2(7514):495–497. doi: 10.1016/s0140-6736(67)91658-3. [DOI] [PubMed] [Google Scholar]
  19. Zucker M. B., Peterson J. Effect of acetylsalicylic acid, other nonsteroidal anti-inflammatory agents, and dipyridamole on human blood platelets. J Lab Clin Med. 1970 Jul;76(1):66–75. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES