Abstract
A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by 14C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.
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Selected References
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- Forsberg C., Rogers H. J. Autolytic enzymes in growth of bacteria. Nature. 1971 Jan 22;229(5282):272–273. doi: 10.1038/229272a0. [DOI] [PubMed] [Google Scholar]
- Hughes R. C. The cell wall of Bacillus licheniformis N.C.T.C. 6346. Isolation of low-molecular-weight fragments from the soluble mucopeptide. Biochem J. 1968 Jan;106(1):49–59. doi: 10.1042/bj1060049. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirelman D., Bracha R., Sharon N. Role of the penicillin-sensitive transpeptidation reaction in attachment of newly synthesized peptidoglycan to cell walls of Micrococcus luteus. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3355–3359. doi: 10.1073/pnas.69.11.3355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirelman D., Sharon N. Biosynthesis of peptidoglycan by a cell wall preparation of Staphylococcus aureus and its inhibition by penicillin. Biochem Biophys Res Commun. 1972 Mar 10;46(5):1909–1917. doi: 10.1016/0006-291x(72)90069-1. [DOI] [PubMed] [Google Scholar]
- Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
- Perkins H. R. Specificity of combination between mucopeptide precursors and vancomycin or ristocetin. Biochem J. 1969 Jan;111(2):195–205. doi: 10.1042/bj1110195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Siewert G., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. XI. Formation of the isoglutamine amide group in the cell walls of Staphylococcus aureus. J Biol Chem. 1968 Feb 25;243(4):783–790. [PubMed] [Google Scholar]
- Ward J. B., Perkins H. R. The direction of glycan synthesis in a bacterial peptidoglycan. Biochem J. 1973 Dec;135(4):721–728. doi: 10.1042/bj1350721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward J. B., Perkins H. R. The purification and properties of two staphylolytic enzymes from Streptomyces griseus. Biochem J. 1968 Jan;106(1):69–76. doi: 10.1042/bj1060069. [DOI] [PMC free article] [PubMed] [Google Scholar]