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. 1974 Jun;139(3):781–784. doi: 10.1042/bj1390781

Peptidoglycan biosynthesis by preparations from Bacillus licheniformis: cross-linking of newly synthesized chains to preformed cell wall (Short Communication)

J Barrie Ward 1, Harold R Perkins 1
PMCID: PMC1166343  PMID: 4853570

Abstract

A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by 14C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Forsberg C., Rogers H. J. Autolytic enzymes in growth of bacteria. Nature. 1971 Jan 22;229(5282):272–273. doi: 10.1038/229272a0. [DOI] [PubMed] [Google Scholar]
  2. Hughes R. C. The cell wall of Bacillus licheniformis N.C.T.C. 6346. Isolation of low-molecular-weight fragments from the soluble mucopeptide. Biochem J. 1968 Jan;106(1):49–59. doi: 10.1042/bj1060049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Mirelman D., Bracha R., Sharon N. Role of the penicillin-sensitive transpeptidation reaction in attachment of newly synthesized peptidoglycan to cell walls of Micrococcus luteus. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3355–3359. doi: 10.1073/pnas.69.11.3355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mirelman D., Sharon N. Biosynthesis of peptidoglycan by a cell wall preparation of Staphylococcus aureus and its inhibition by penicillin. Biochem Biophys Res Commun. 1972 Mar 10;46(5):1909–1917. doi: 10.1016/0006-291x(72)90069-1. [DOI] [PubMed] [Google Scholar]
  5. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  6. Perkins H. R. Specificity of combination between mucopeptide precursors and vancomycin or ristocetin. Biochem J. 1969 Jan;111(2):195–205. doi: 10.1042/bj1110195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Siewert G., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. XI. Formation of the isoglutamine amide group in the cell walls of Staphylococcus aureus. J Biol Chem. 1968 Feb 25;243(4):783–790. [PubMed] [Google Scholar]
  8. Ward J. B., Perkins H. R. The direction of glycan synthesis in a bacterial peptidoglycan. Biochem J. 1973 Dec;135(4):721–728. doi: 10.1042/bj1350721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ward J. B., Perkins H. R. The purification and properties of two staphylolytic enzymes from Streptomyces griseus. Biochem J. 1968 Jan;106(1):69–76. doi: 10.1042/bj1060069. [DOI] [PMC free article] [PubMed] [Google Scholar]

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