Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Oct;144(1):141–148. doi: 10.1042/bj1440141

Changes in respiratory activities during the cell-cycle of the fission yeast Schizosaccharomyces pombe 972h− growing in the presence of glycerol

Robert K Poole 1,*, David Lloyd 1
PMCID: PMC1168474  PMID: 4156830

Abstract

1. The specific activities of cytochrome c oxidase, catalase, succinate dehydrogenase, succinate–cytochrome c oxidoreductase, NADH–cytochrome c oxidoreductase, and NADPH–cytochrome c oxidoreductase in mid-exponential-phase batch cultures of glycerol-grown Schizosaccharomyces pombe indicated that the organisms were catabolite-de-repressed. 2. In cultures growing synchronously in the presence of glycerol as sole carbon source, the respiration rate showed two abrupt increases at about 0.45 and 0.95 of the cell-cycle and remained constant in the periods between successive rises. 3. Catalase, succinate dehydrogenase, NADH–cytochrome c oxidoreductase and acid p-nitrophenyl-phosphatase all showed peak patterns of expression in synchronous cultures. 4. Cytochrome c oxidase and cytochromes a+a3 both showed step patterns of expression with two rises per cell-cycle. 5. Cytochromes c548, b554 and b560 all followed similar time-courses in step patterns of expression, but these were distinct from, and more complex than, that of cytochromes a+a3. 6. These results are compared with those previously obtained with glucose-grown cultures, and the part played by catabolite repression in the expression of respiratory activities in the cell-cycle is assessed.

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLUMENTHAL L. K., ZAHLER S. A. Index for measurement of synchronization of cell populations. Science. 1962 Mar 2;135(3505):724–724. doi: 10.1126/science.135.3505.724. [DOI] [PubMed] [Google Scholar]
  2. Dharmalingam K., Jayaraman J. Mitochondriogenesis in synchronous cultures of yeast. I. Oscillatory pattern of respiration. Arch Biochem Biophys. 1973 Jul;157(1):197–202. doi: 10.1016/0003-9861(73)90405-0. [DOI] [PubMed] [Google Scholar]
  3. GARLAND P. B., RANDLE P. J. A rapid enzymatic assay for glycerol. Nature. 1962 Dec 8;196:987–988. doi: 10.1038/196987a0. [DOI] [PubMed] [Google Scholar]
  4. Halvorson H. O., Carter B. L., Tauro P. Synthesis of enzymes during the cell cycle. Adv Microb Physiol. 1971;6(0):47–106. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Linnane A. W., Haslam J. M., Lukins H. B., Nagley P. The biogenesis of mitochondria in microorganisms. Annu Rev Microbiol. 1972;26:163–198. doi: 10.1146/annurev.mi.26.100172.001115. [DOI] [PubMed] [Google Scholar]
  7. MILNER H. W., LAWRENCE N. S., FRENCH C. S. Colloidal dispersion of chloroplast material. Science. 1950 Jun 9;111(2893):633–634. doi: 10.1126/science.111.2893.633. [DOI] [PubMed] [Google Scholar]
  8. Marchant R. The initiation of cell wall synthesis in parasynchronous cultures of Schizosaccharomyces pombe. Arch Mikrobiol. 1971;78(3):205–213. doi: 10.1007/BF00424894. [DOI] [PubMed] [Google Scholar]
  9. Mitchison J. M., Creanor J. Linear synthesis of sucrase and phosphatases during the cell cycle of Schizosaccharomyces pombe. J Cell Sci. 1969 Sep;5(2):373–391. doi: 10.1242/jcs.5.2.373. [DOI] [PubMed] [Google Scholar]
  10. Osumi M., Sando N. Division of yeast mitochondria in synchronous culture. J Electron Microsc (Tokyo) 1969;18(1):47–56. [PubMed] [Google Scholar]
  11. Poole R. K., Lloyd D., Chance B. The development of cytochromes during the cell cycle of a glucose-repressed fission yeast, Schizosaccharomyces pombe 972h-. Biochem J. 1974 Feb;138(2):201–210. doi: 10.1042/bj1380201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Poole R. K., Lloyd D. Oscillations of enzyme activities during the cell-cycle of a glucose-repressed fission-yeast Schizosaccharomyces pombe 972h-. Biochem J. 1973 Sep;136(1):195–207. doi: 10.1042/bj1360195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SCOPES A. W., WILLIAMSON D. H. THE GROWTH AND OXYGEN UPTAKE OF SYNCHRONOUSLY DIVIDING CULTURES OF SACCHAROMYCES CEREVISIAE. Exp Cell Res. 1964 Jul;35:361–371. doi: 10.1016/0014-4827(64)90102-8. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES