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. 1975 Dec;151(3):751–753. doi: 10.1042/bj1510751

A gamma Bence-Jones protein in guinea pigs.

F K Stevenson, L E Mole, C M Raymont, G T Stevenson
PMCID: PMC1172425  PMID: 814896

Abstract

1. The L2C lymphocytic leukaemia in strain-2 guinea pigs is accompanied by a protein in the urine resembling a homogenous immunoglobulin light chain. 2. The amino acid sequence over the first 20 residues demonstrates a close analogy with a human gamma chain of V region subgroup IV. 3. The protein is apparently synthesized by the leukaemic cells and thus represents a monoclonal light chain, i.e. a Bence-Jones protein.

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Selected References

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  1. Awdeh Z. L., Williamson A. R., Askonas B. A. One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins. Biochem J. 1970 Jan;116(2):241–248. doi: 10.1042/bj1160241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERNIER G. M., PUTNAM F. W. MONOMER--DIMER FORMS OF BENCE JONES PROTEINS. Nature. 1963 Oct 19;200:223–225. doi: 10.1038/200223b0. [DOI] [PubMed] [Google Scholar]
  3. Cohen S., Porter R. R. Heterogeneity of the peptide chains of gamma-globulin. Biochem J. 1964 Feb;90(2):278–284. doi: 10.1042/bj0900278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deutsch H. F. Relationships of two light chain immunoglobulins isolated from the same human source (low molecular weight immunoglobins). Immunochemistry. 1965 Sep;2(3):207–218. doi: 10.1016/0019-2791(65)90002-9. [DOI] [PubMed] [Google Scholar]
  5. FAHEY J. L. CONTRIBUTION OF GAMMA-GLOBULIN SUBUNITS TO ELECTROPHORETIC HETEROGENEITY: IDENTIFICATION OF A DISTINCTIVE GROUP OF 6.6S GAMMA-MYELOMA PROTEINS. Immunochemistry. 1964 Jun;1:121–131. doi: 10.1016/0019-2791(64)90076-x. [DOI] [PubMed] [Google Scholar]
  6. GALLY J. A., EDELMAN G. M. PROTEIN-PROTEIN INTERACTIONS AMONG L POLYPEPTIDE CHAINS OF BENCE-JONES PROTEINS AND HUMAN GAMMA-GLOBULINS. J Exp Med. 1964 May 1;119:817–836. doi: 10.1084/jem.119.5.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MONTGOMERY R. Further studies of the phenol-sulfuric acid reagent for carbohydrates. Biochim Biophys Acta. 1961 Apr 15;48:591–593. doi: 10.1016/0006-3002(61)90059-2. [DOI] [PubMed] [Google Scholar]
  9. Nadel E., Liu P., Burstein S. Experimental perinatal leukemia in guinea pigs. Beitr Pathol. 1974 Mar;151(3):268–280. doi: 10.1016/s0005-8165(74)80004-1. [DOI] [PubMed] [Google Scholar]
  10. PUTNAM F. W., EASLEY C. W., LYNN L. T., RITCHIE A. E., PHELPS R. A. The heat precipitation of Bence-Jones proteins. I. Optimum conditions. Arch Biochem Biophys. 1959 Jul;83(1):115–130. doi: 10.1016/0003-9861(59)90016-5. [DOI] [PubMed] [Google Scholar]
  11. STEVENSON G. T. Detection in normal urine of protein resembling Bence Jones protein. J Clin Invest. 1960 Jul;39:1192–1200. doi: 10.1172/JCI104134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shevach E. M., Ellman L., Davie J. M., Green I. L2C Guinea pig lymphatic leukemia: a "B" cell leukemia. Blood. 1972 Jan;39(1):1–12. [PubMed] [Google Scholar]
  13. Stevenson G. T., Eady R. P., Hough D. W., Jurd R. D., Stevenson F. K. Surface immunoglobulin of guinea-pig leukaemic lymphocytes. Immunology. 1975 May;28(5):807–820. [PMC free article] [PubMed] [Google Scholar]
  14. Summers M. R., Smythers G. W., Oroszlan S. Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets. Anal Biochem. 1973 Jun;53(2):624–628. doi: 10.1016/0003-2697(73)90114-0. [DOI] [PubMed] [Google Scholar]
  15. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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