Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1975 Dec;151(3):751–753. doi: 10.1042/bj1510751

A gamma Bence-Jones protein in guinea pigs.

F K Stevenson, L E Mole, C M Raymont, G T Stevenson
PMCID: PMC1172425  PMID: 814896

Abstract

1. The L2C lymphocytic leukaemia in strain-2 guinea pigs is accompanied by a protein in the urine resembling a homogenous immunoglobulin light chain. 2. The amino acid sequence over the first 20 residues demonstrates a close analogy with a human gamma chain of V region subgroup IV. 3. The protein is apparently synthesized by the leukaemic cells and thus represents a monoclonal light chain, i.e. a Bence-Jones protein.

Full text

PDF
753

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Awdeh Z. L., Williamson A. R., Askonas B. A. One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins. Biochem J. 1970 Jan;116(2):241–248. doi: 10.1042/bj1160241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERNIER G. M., PUTNAM F. W. MONOMER--DIMER FORMS OF BENCE JONES PROTEINS. Nature. 1963 Oct 19;200:223–225. doi: 10.1038/200223b0. [DOI] [PubMed] [Google Scholar]
  3. Cohen S., Porter R. R. Heterogeneity of the peptide chains of gamma-globulin. Biochem J. 1964 Feb;90(2):278–284. doi: 10.1042/bj0900278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deutsch H. F. Relationships of two light chain immunoglobulins isolated from the same human source (low molecular weight immunoglobins). Immunochemistry. 1965 Sep;2(3):207–218. doi: 10.1016/0019-2791(65)90002-9. [DOI] [PubMed] [Google Scholar]
  5. FAHEY J. L. CONTRIBUTION OF GAMMA-GLOBULIN SUBUNITS TO ELECTROPHORETIC HETEROGENEITY: IDENTIFICATION OF A DISTINCTIVE GROUP OF 6.6S GAMMA-MYELOMA PROTEINS. Immunochemistry. 1964 Jun;1:121–131. doi: 10.1016/0019-2791(64)90076-x. [DOI] [PubMed] [Google Scholar]
  6. GALLY J. A., EDELMAN G. M. PROTEIN-PROTEIN INTERACTIONS AMONG L POLYPEPTIDE CHAINS OF BENCE-JONES PROTEINS AND HUMAN GAMMA-GLOBULINS. J Exp Med. 1964 May 1;119:817–836. doi: 10.1084/jem.119.5.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MONTGOMERY R. Further studies of the phenol-sulfuric acid reagent for carbohydrates. Biochim Biophys Acta. 1961 Apr 15;48:591–593. doi: 10.1016/0006-3002(61)90059-2. [DOI] [PubMed] [Google Scholar]
  9. Nadel E., Liu P., Burstein S. Experimental perinatal leukemia in guinea pigs. Beitr Pathol. 1974 Mar;151(3):268–280. doi: 10.1016/s0005-8165(74)80004-1. [DOI] [PubMed] [Google Scholar]
  10. PUTNAM F. W., EASLEY C. W., LYNN L. T., RITCHIE A. E., PHELPS R. A. The heat precipitation of Bence-Jones proteins. I. Optimum conditions. Arch Biochem Biophys. 1959 Jul;83(1):115–130. doi: 10.1016/0003-9861(59)90016-5. [DOI] [PubMed] [Google Scholar]
  11. STEVENSON G. T. Detection in normal urine of protein resembling Bence Jones protein. J Clin Invest. 1960 Jul;39:1192–1200. doi: 10.1172/JCI104134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shevach E. M., Ellman L., Davie J. M., Green I. L2C Guinea pig lymphatic leukemia: a "B" cell leukemia. Blood. 1972 Jan;39(1):1–12. [PubMed] [Google Scholar]
  13. Stevenson G. T., Eady R. P., Hough D. W., Jurd R. D., Stevenson F. K. Surface immunoglobulin of guinea-pig leukaemic lymphocytes. Immunology. 1975 May;28(5):807–820. [PMC free article] [PubMed] [Google Scholar]
  14. Summers M. R., Smythers G. W., Oroszlan S. Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets. Anal Biochem. 1973 Jun;53(2):624–628. doi: 10.1016/0003-2697(73)90114-0. [DOI] [PubMed] [Google Scholar]
  15. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES