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. 1972 Nov;130(1):55–62. doi: 10.1042/bj1300055

Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310

J Melling 1,2, G K Scott 1,2
PMCID: PMC1174300  PMID: 4570347

Abstract

Purified penicillinase, in gram quantities, has been prepared from Escherichia coli strain W3310 by using methods developed to handle large amounts of material. The final product had a specific enzyme activity of 3.08 units/μg of protein, which was over twice as high as that reported previously (Datta & Richmond, 1966). The purified enzyme was similar to that from E. coli strain TEM, but different in molecular weight and some other respects. The differences observed may be a result of the greater purity obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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