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. 1973 Feb;131(2):199–209. doi: 10.1042/bj1310199

Palmitoyl-coenzyme A synthetase. Mechanism of reaction

J Bar-Tana 1, G Rose 1, R Brandes 1, B Shapiro 1
PMCID: PMC1177459  PMID: 4722436

Abstract

The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. 18O was transferred from [18O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bar-Tana J., Ben-Zeev O., Rose G., Deutsh J. Determination of 18 O content of phosphate compounds--a novel method. Biochim Biophys Acta. 1972 Mar 30;264(1):214–219. [PubMed] [Google Scholar]
  2. Bar-Tana J., Rose G., Shapiro B. Microsomal palmitoyl coenzyme A synthetase from rat liver. Partial and exchange reactions. Biochem J. 1972 Oct;129(5):1101–1107. doi: 10.1042/bj1291101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bar-Tana J., Rose G., Shapiro B. The purification and properties of microsomal palmitoyl-coenzyme A synthetase. Biochem J. 1971 Apr;122(3):353–362. doi: 10.1042/bj1220353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CLELAND W. W. The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations. Biochim Biophys Acta. 1963 Jan 8;67:104–137. doi: 10.1016/0006-3002(63)91800-6. [DOI] [PubMed] [Google Scholar]
  5. CLELAND W. W. The kinetics of enzyme-catalyzed reactions with two or more substrates or products. III. Prediction of initial velocity and inhibition patterns by inspection. Biochim Biophys Acta. 1963 Feb 12;67:188–196. doi: 10.1016/0006-3002(63)91816-x. [DOI] [PubMed] [Google Scholar]
  6. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MEISTER A., KRISHNASWAMY P. R., PAMILJANS V. Mechanism of glutamic acid activation and glutamine synthesis. Fed Proc. 1962 Nov-Dec;21:1013–1022. [PubMed] [Google Scholar]

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