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. 1973 Feb;131(2):199–209. doi: 10.1042/bj1310199

Palmitoyl-coenzyme A synthetase. Mechanism of reaction

J Bar-Tana 1, G Rose 1, R Brandes 1, B Shapiro 1
PMCID: PMC1177459  PMID: 4722436

Abstract

The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. 18O was transferred from [18O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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