Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Jul;118(4):557–561. doi: 10.1042/bj1180557

Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis

J E Walker 1,*, E P Abraham 1
PMCID: PMC1179251  PMID: 4991475

Abstract

1. Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained. 2. The process involves adsorption on a cation-exchange resin and elution with aqueous trimethylamine, separation from neutral amino acids and glutamic acid by chromatography on DEAE-Sephadex at pH8.7 and separation from other neutral peptides by chromatography in aqueous propan-2-ol on Sephadex G-25. 3. A new amino acid, which is chemically related to bacilysin, was isolated from the fraction containing neutral amino acids. 4. Two substances that yield alanine on hydrolysis, in addition to bacilysin, were obtained from the neutral peptide fraction.

Full text

PDF
561

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARNEGIE P. R. A method for separating neutral amino acids from neutral oligopeptides. Biochem J. 1961 Apr;78:687–691. doi: 10.1042/bj0780687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CARNEGIE P. R. Separation of low molecular weight peptides from amino-acids on 'DEAE-Sephadex'. Nature. 1961 Nov 18;192:658–659. doi: 10.1038/192658a0. [DOI] [PubMed] [Google Scholar]
  3. LOCKHART I. M., ABRAHAM E. P. The amino acid sequence in bacitracin A. Biochem J. 1954 Dec;58(4):633–647. doi: 10.1042/bj0580633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MITCHELL P. A new technique for stirred aerated culture. Nature. 1949 Nov 12;164(4176):846–846. doi: 10.1038/164846a0. [DOI] [PubMed] [Google Scholar]
  5. POLLOCK M. R., KRAMER M. Intermediates in the biosynthesis of bacterial penicillinase. Biochem J. 1958 Dec;70(4):665–681. doi: 10.1042/bj0700665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. POLLOCK M. R. Penicillinase adaptation and fixation of penicillin sulphur by Bacillus cereus spores. J Gen Microbiol. 1953 Feb;8(1):186–194. doi: 10.1099/00221287-8-1-186. [DOI] [PubMed] [Google Scholar]
  7. Rogers H. J., Lomakina N., Abraham E. P. Observations on the structure of bacilysin. Biochem J. 1965 Nov;97(2):579–586. doi: 10.1042/bj0970579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rogers H. J., Newton G. G., Abraham E. P. Production and purification of bacilysin. Biochem J. 1965 Nov;97(2):573–578. doi: 10.1042/bj0970573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roscoe J., Abraham E. P. Experiments relating to the biosynthesis of bacilysin. Biochem J. 1966 Jun;99(3):793–800. doi: 10.1042/bj0990793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walker J. E., Abraham E. P. The structure of bacilysin and other products of Bacillus subtilis. Biochem J. 1970 Jul;118(4):563–570. doi: 10.1042/bj1180563. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES