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. 1978 Jan 1;169(1):79–85. doi: 10.1042/bj1690079

Localization of the ribonucleotide sites in rat liver mitochondrial deoxyribonucleic acid.

D M Lonsdale, I G Jones
PMCID: PMC1184196  PMID: 629755

Abstract

Supercoiled rat liver mitochondrial DNA is relaxed by treatment with ribonucleases A, T1 or H. All the supercoiled mitochondrial DNA is sensitive to ribonuclease H and ribonuclease A, but only 35% of the supercoiled population is sensitive to ribonuclease T1. Removal of the ribonucleotides with calf thymus ribonuclease H, followed by denaturation of the mitochondrial DNA and analysis of the single-strand fragment lengths in the electron microscope, showed that the ribonucleotides were randomly located on both strands of the DNA. Endonuclease-S1 digestion of mitochondrial DNA after removal of the ribonucleotides reveals that no unique fragments are produced and ribonucleotides are randomly distributed with respect to one another. The average number of ribonucleotide sites per molecule was estimated to be between 8 and 13. Two possible mechanisms for the origin of ribonucleotide sites are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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