Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Feb;116(4):563–567. doi: 10.1042/bj1160563

Characterization of rapidly labelled rat liver ribonucleic acid showing high affinity for columns of methylated albumin on kieselguhr

W Kunz 1, J Niessing 1, B Schnieders 1, C E Sekeris 1
PMCID: PMC1185400  PMID: 4314130

Abstract

Most of the rapidly labelled RNA from rat liver submitted to column chromatography on methylated albumin on kieselguhr remains tightly bound to the column and can only be recovered by elution with m-ammonia. The tightly bound RNA is composed mainly of DNA-like RNA. The binding capacity is dependent not only on base composition but also on molecular size: the heavier RNA molecules show a greater affinity to the column than do the lower-molecular-weight components. Rapidly labelled mouse liver and Saccharomyces cerevisiae RNA show similar behaviour to rat liver RNA on columns of methylated albumin on kieselguhr.

Full text

PDF
567

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billing R. J., Inglis A. M., Smellie R. M. The distribution of deoxyribonucleic acid-like ribonucleic acid in rat liver cells. Biochem J. 1969 Jul;113(3):571–572. doi: 10.1042/bj1130571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ellem K. A., Sheridan J. W. Tenacious binding of the bulk of the DNA-like RNA of metazoan cells to methylated albumin columns. Biochem Biophys Res Commun. 1964 Aug 11;16(6):505–510. doi: 10.1016/0006-291x(64)90183-4. [DOI] [PubMed] [Google Scholar]
  3. Ellem K. A. Some properties of mammalian DNA-like RNA isolated by chromatography on methylated bovine serum albumin-kieselguhr columns. J Mol Biol. 1966 Sep;20(2):283–305. doi: 10.1016/0022-2836(66)90065-9. [DOI] [PubMed] [Google Scholar]
  4. GEORGIEV G. P., MANTIEVA V. L. The isolation of DNA-like RNA and ribosomal RNA from the nucleolo-chromosomal apparatus of mammalian cells. Biochim Biophys Acta. 1962 Jul 9;61:153–154. doi: 10.1016/0926-6550(62)90046-4. [DOI] [PubMed] [Google Scholar]
  5. GEORGIEV G. P., SAMARINA O. P., LERMAN M. I., SMIRNOV M. N., SEVERTZOV A. N. BIOSYNTHESIS OF MESSENGER AND RIBOSOMAL RIBONUCLEIC ACIDS IN THE NUCLEOLOCHROMOSOMAL APPARATUS OF ANIMAL CELLS. Nature. 1963 Dec 28;200:1291–1294. doi: 10.1038/2001291a0. [DOI] [PubMed] [Google Scholar]
  6. Geibel U., Hennig W., Kunz W., Schneiders B. Stimulierung der Biosynthese von Ribonucleinsäuren der Leber durch Phenobarbital. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1968;260(2):118–119. [PubMed] [Google Scholar]
  7. KOCH G., KUBINSKI H. ZUR DARSTELLUNG UND FRAKTIONIERUNG VON HOCHMOLEKULARER RIBONUCLEINSAEURE. Z Naturforsch B. 1964 Aug;19:683–687. [PubMed] [Google Scholar]
  8. Lang N., Sekeris C. E. Zum Wirkungsmechanismus der Hormone. 3. Einfluss von Cortisol auf den Ribonucleinsäureund Proteinstoffwechsel in Rattenleber. Hoppe Seylers Z Physiol Chem. 1964;339(1):238–248. [PubMed] [Google Scholar]
  9. Lingrel J. B. Studies on the rapidly labeled RNA of rabbit bone marrow cells. Biochim Biophys Acta. 1967 Jun 20;142(1):75–88. doi: 10.1016/0005-2787(67)90516-3. [DOI] [PubMed] [Google Scholar]
  10. STAEHELIN T., WETTSTEIN F. O., OURA H., NOLL H. DETERMINATION OF THE CODING RATIO BASED ON MOLECULAR WEIGHT OF MESSENGER RIBONUCLEIC ACID ASSOCIATED WITH ERGOSOMES OF DIFFERENT AGGREGATE SIZE. Nature. 1964 Jan 18;201:264–270. doi: 10.1038/201264a0. [DOI] [PubMed] [Google Scholar]
  11. Schütz G., Gallwitz D., Sekeris C. E. Rapidly labelled high molecular RNA from rat liver. Eur J Biochem. 1968 Apr 3;4(2):149–156. doi: 10.1111/j.1432-1033.1968.tb00186.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES