Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Mar;116(5):791–796. doi: 10.1042/bj1160791

Subunits of Tamm–Horsfall glycoprotein

F K Stevenson 1, P W Kent 1
PMCID: PMC1185500  PMID: 5441368

Abstract

1. The effect of 6m-guanidine hydrochloride on the urinary glycoprotein described by Tamm & Horsfall (1952) is to produce a homogeneous subunit of molecular weight approx. 100000. 2. Complete reduction of the disulphide bonds of this subunit does not decrease the molecular weight, suggesting that all disulphide bonds are intrachain. 3. Comparison of sedimentation and viscometric behaviour of unreduced and reduced material in 6m-guanidine hydrochloride is consistent with reduction causing an opening-up of intrachain disulphide bonds to give a more asymmetric molecule.

Full text

PDF
793

Images in this article

793Fig. 1.
on p.793
793Fig. 2.
on p.793
794Fig. 3.
on p.794

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CURTAIN C. C. The viscometric behaviour of a mucoprotein isolated from human urine. Aust J Exp Biol Med Sci. 1953 Jun;31(3):255–265. doi: 10.1038/icb.1953.30. [DOI] [PubMed] [Google Scholar]
  2. FERRIS T. G., EASTERLING R. E., BUDD R. E. Electrophoresis of serum proteins in acrylamide gel. I. Removal of background stain by means of electric current. Am J Clin Pathol. 1962 Oct;38:383–387. doi: 10.1093/ajcp/38.4.383. [DOI] [PubMed] [Google Scholar]
  3. Friedmann T., Johnson P. Further characterization of the subunit of Tamm-Horsfall mucoprotein. Biochim Biophys Acta. 1966 Dec 28;130(2):355–360. doi: 10.1016/0304-4165(66)90231-5. [DOI] [PubMed] [Google Scholar]
  4. Friedmann T., Johnson P. The disaggregation of Tamm Horsfall mucroprotein by acetic acid. Biochim Biophys Acta. 1966 Jun 29;121(2):292–297. doi: 10.1016/0304-4165(66)90118-8. [DOI] [PubMed] [Google Scholar]
  5. Kawahara K., Tanford C. The number of polypeptide chains in rabbit muscle aldolase. Biochemistry. 1966 May;5(5):1578–1584. doi: 10.1021/bi00869a018. [DOI] [PubMed] [Google Scholar]
  6. Kawahara K., Tanford C. Viscosity and density of aqueous solutions of urea and guanidine hydrochloride. J Biol Chem. 1966 Jul 10;241(13):3228–3232. [PubMed] [Google Scholar]
  7. McQueen E. G., Engel G. B. Factors determining the aggregation of urinary mucoprotein. J Clin Pathol. 1966 Jul;19(4):392–396. doi: 10.1136/jcp.19.4.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PORTER K. R., TAMM I. Direct visualization of a mucoprotein component of urine. J Biol Chem. 1955 Jan;212(1):135–140. [PubMed] [Google Scholar]
  9. Stevenson F. K. The viscosity of Tamm-Horsfall mucoprotein and its relation to cystic fibrosis. Clin Chim Acta. 1969 Mar;23(3):441–447. doi: 10.1016/0009-8981(69)90346-5. [DOI] [PubMed] [Google Scholar]
  10. Stevenson F. K. The viscosity of Tamm-Horsfall mucoprotein. Biochim Biophys Acta. 1968 Jun 26;160(2):296–298. doi: 10.1016/0005-2795(68)90111-6. [DOI] [PubMed] [Google Scholar]
  11. Stevenson G. T., Straus D. Specific dimerization of the light chains of human immunoglobulin. Biochem J. 1968 Jul;108(3):375–382. doi: 10.1042/bj1080375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tanford C. Protein denaturation. Adv Protein Chem. 1968;23:121–282. doi: 10.1016/s0065-3233(08)60401-5. [DOI] [PubMed] [Google Scholar]
  13. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  14. YPHANTIS D. A. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. Biochemistry. 1964 Mar;3:297–317. doi: 10.1021/bi00891a003. [DOI] [PubMed] [Google Scholar]
  15. Zimm B. H., Crothers D. M. SIMPLIFIED ROTATING CYLINDER VISCOMETER FOR DNA. Proc Natl Acad Sci U S A. 1962 Jun;48(6):905–911. doi: 10.1073/pnas.48.6.905. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES