Abstract
The oxygen enzymically inserted as a hydroxy function by rat liver post-mitochondrial fraction into the 25-position of cholecalciferol to giver 25-hydroxycholecaliferol is derived exclusively from molecular O2. Therefore like the other two cholecalciferol hydroxylases, i.e. 25-hydroxycholecalciferol 1alpha-hydroxylase and 25-hydroxycholecalciferol 24-hydroxylase, the cholecalciferol 25-hydroxylase is also a mono-oxygenase ('mixed-function oxidase').
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Selected References
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- Bhattacharyya M. H., DeLuca H. F. Subcellular location of rat liver calciferol-25-hydroxylase. Arch Biochem Biophys. 1974 Jan;160(1):58–62. doi: 10.1016/s0003-9861(74)80008-1. [DOI] [PubMed] [Google Scholar]
- Blunt J. W., DeLuca H. F., Schnoes H. K. 25-hydroxycholecalciferol. A biologically active metabolite of vitamin D3. Biochemistry. 1968 Oct;7(10):3317–3322. doi: 10.1021/bi00850a001. [DOI] [PubMed] [Google Scholar]
- DeLuca H. F. Vitamin D: the vitamin and the hormone. Fed Proc. 1974 Nov;33(11):2211–2219. [PubMed] [Google Scholar]
- Ghazarian J. G., DeLuca H. F. 25-Hydroxycholecalciferol-1-hydroxylase: a specific requirement for NADPH and a hemoprotein component in chick kidney mitochondria. Arch Biochem Biophys. 1974 Jan;160(1):63–72. doi: 10.1016/s0003-9861(74)80009-3. [DOI] [PubMed] [Google Scholar]
- Ghazarian J. G., Jefcoate C. R., Knutson J. C., Orme-Johnson W. H., DeLuca H. F. Mitochondrial cytochrome p450. A component of chick kidney 25-hydrocholecalciferol-1alpha-hydroxylase. J Biol Chem. 1974 May 25;249(10):3026–3033. [PubMed] [Google Scholar]
- Ghazarian J. G., Schnoes H. K., DeLuca H. F. Mechanism of 25-hydroxycholecalciferol 1 -hydroxylation. Incorporation of oxygen-18 into the 1 position of 25-hydroxycholecalciferol. Biochemistry. 1973 Jul 3;12(14):2555–2558. doi: 10.1021/bi00738a001. [DOI] [PubMed] [Google Scholar]
- Holick M. F., DeLuca H. F. A new chromatographic system for vitamin D3 and its metabolites: resoluation of a new vitamin D3 metabolite. J Lipid Res. 1971 Jul;12(4):460–465. [PubMed] [Google Scholar]
- Horsting M., DeLuca H. F. In vitro production of 25-hydroxycholecalciferol. Biochem Biophys Res Commun. 1969 Jul 23;36(2):251–256. doi: 10.1016/0006-291x(69)90322-2. [DOI] [PubMed] [Google Scholar]
- Madhok T. C., Schnoes H. K., DeLuca H. F. Mechanism of 25-hydroxyvitamin D3 24-hydroxylation: incorporation of oxygen-18 into the 24 position of 25-hydroxyvitamin D3. Biochemistry. 1977 May 17;16(10):2142–2145. doi: 10.1021/bi00629a015. [DOI] [PubMed] [Google Scholar]
- Mason J. B., Hay R. W., Leresche J., Peel S., Darley S. The story of vitamin D: from vitamin to hormone. Lancet. 1974 Mar 2;1(7853):325–329. [PubMed] [Google Scholar]
- Olson E. B., Jr, Knutson J. C., Bhattacharyya M. H., DeLuca H. F. The effect of hepatectomy on the synthesis of 25-hydroxyvitamin D3. J Clin Invest. 1976 May;57(5):1213–1220. doi: 10.1172/JCI108389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pedersen J. I., Ghazarian J. G., Orme-Johnson N. R., DeLuca H. F. Isolation of chick renal mitochondrial ferredoxin active in the 25-hydroxyvitamin D3-1alpha-hydroxylase system. J Biol Chem. 1976 Jul 10;251(13):3933–3941. [PubMed] [Google Scholar]
- Ponchon G., Kennan A. L., DeLuca H. F. "Activation" of vitamin D by the liver. J Clin Invest. 1969 Nov;48(11):2032–2037. doi: 10.1172/JCI106168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tucker G., 3rd, Gagnon R. E., Haussler M. R. Vitamin D 3 -25-hydroxylase: tissue occurrence and apparent lack of regulation. Arch Biochem Biophys. 1973 Mar;155(1):47–57. doi: 10.1016/s0003-9861(73)80008-6. [DOI] [PubMed] [Google Scholar]