Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1968 Sep;109(3):325–332. doi: 10.1042/bj1090325

The mechanism of thyrotrophin action in relation to lipid metabolism in thyroid tissue

T W Scott 1, S C Mills 1, N Freinkel 1
PMCID: PMC1186826  PMID: 4301037

Abstract

1. The effects of thyrotrophin in vitro on the incorporation of [14C]-glucose, -glycerol, -palmitate and -oleate into the lipids of thyroid tissue were examined. 2. Thyrotrophin increased the incorporation of these 14C-labelled precursors into phosphatidylinositol specifically. 3. Thyrotrophin also increased the proportion of 14C radioactivity from labelled glucose, glycerol, palmitate and oleate incorporated into the 1,2-diglycerides. 4. The addition of thyrotrophin to thyroid slices for 10min., after 2hr. of prelabelling with [14C]glycerol, also increased the proportion of 14C radioactivity incorporated into the 1,2-diglyceride fraction. 5. After incubation of thyroid tissue with [1-14C]palmitate, thyrotrophin caused a two- to three-fold increase in the specific radioactivity of palmitate isolated from phosphatidylinositol and 1,2-diglycerides. In contrast, the specific radioactivity of palmitate isolated from the choline and ethanolamine phosphoglycerides, 1,3-diglycerides and triglycerides was not increased by thyrotrophin.

Full text

PDF
329

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COLLINS F. D. STUDIES ON PHOSPHOLIPIDS. 9. THE COMPOSITION OF RAT-LIVER LECITHINS. Biochem J. 1963 Aug;88:319–324. doi: 10.1042/bj0880319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAWSON R. M., HEMINGTON N., DAVENPORT J. B. Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses. Biochem J. 1962 Sep;84:497–501. doi: 10.1042/bj0840497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. FREINKEL N. Further observations concerning the action of pituitary thyrotropin on the intermediate metabolism of sheep thyroid tissue in vitro. Endocrinology. 1960 Jun;66:851–859. doi: 10.1210/endo-66-6-851. [DOI] [PubMed] [Google Scholar]
  5. FREINKEL N. Pathways of thyroid phosphorus metabolism; the phospholipids of sheep thyroid. Biochem J. 1958 Feb;68(2):327–333. doi: 10.1042/bj0680327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FREINKEL N. Pathways of thyroidal phosphorus metabolism: the effect of pituitary thyrotropin upon the phospholipids of the sheep thyroid gland. Endocrinology. 1957 Oct;61(4):448–460. doi: 10.1210/endo-61-4-448. [DOI] [PubMed] [Google Scholar]
  7. FREINKEL N., SCOTT T. W. EFFECT OF PITUITARY THYROTROPHIN ON THE DIGLYCERIDE TRIGLYCERIDE INTERRELATIONSHIPS IN THYROID TISSUE. Nature. 1964 Dec 26;204:1313–1315. doi: 10.1038/2041313a0. [DOI] [PubMed] [Google Scholar]
  8. GOLDRICK B., HIRSCH J. A TECHNIQUE FOR QUANTITATIVE RECOVERY OF LIPIDS FROM CHROMATOPLATES. J Lipid Res. 1963 Oct;4:482–483. [PubMed] [Google Scholar]
  9. HANAHAN D. J., OLLEY J. N. Chemical nature of monophosphoinositides. J Biol Chem. 1958 Apr;231(2):813–828. [PubMed] [Google Scholar]
  10. HOKIN L. E., HOKIN M. R. BIOLOGICAL TRANSPORT. Annu Rev Biochem. 1963;32:553–578. doi: 10.1146/annurev.bi.32.070163.003005. [DOI] [PubMed] [Google Scholar]
  11. HOKIN L. E., HOKIN M. R. Diglyceride kinase and other path ways for phosphatidic acid synthesis in the erythrocyte membrane. Biochim Biophys Acta. 1963 Mar 12;67:470–484. doi: 10.1016/0006-3002(63)91852-3. [DOI] [PubMed] [Google Scholar]
  12. HOKIN L. E., HOKIN M. R. The actions of pancreozymin in pancreas slices and the role of phospholipids in enzyme secretion. J Physiol. 1956 May 28;132(2):442–453. doi: 10.1113/jphysiol.1956.sp005536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOKIN M. R., BENFEY B. G., HOKIN L. E. Phospholipides and adrenaline secretion in guinea pig adrenal medulla. J Biol Chem. 1958 Oct;233(4):814–817. [PubMed] [Google Scholar]
  14. HOKIN M. R., HOKIN L. E., SAFFRAN M., SCHALLY A. V., ZIMMERMANN B. U. Phospholipides and the secretion of adrenocorticotropin and of corticosteroids. J Biol Chem. 1958 Oct;233(4):811–813. [PubMed] [Google Scholar]
  15. Hokin L. E., Huebner D. Radioautographic localization of the increased synthesis of phosphatidylinositol in response to pancreozymin or acetylcholine in guinea pig pancreas slices. J Cell Biol. 1967 Jun;33(3):521–530. doi: 10.1083/jcb.33.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KARNOVSKY M. L. Metabolic basis of phagocytic activity. Physiol Rev. 1962 Jan;42:143–168. doi: 10.1152/physrev.1962.42.1.143. [DOI] [PubMed] [Google Scholar]
  17. KEENAN R. W., HOKIN L. E. THE ENZYMATIC ACYLATION OF LYSOPHOSPHATIDYLINOSITOL. J Biol Chem. 1964 Jul;239:2123–2129. [PubMed] [Google Scholar]
  18. KENNEDY E. P. Biosynthesis of complex lipids. Fed Proc. 1961 Dec;20:934–940. [PubMed] [Google Scholar]
  19. Kessler J. I., Demeny M., Sobotka H. Rates of tissue uptake of palmitic acid-1-14C complexed with albumin by two different procedures. J Lipid Res. 1967 May;8(3):185–190. [PubMed] [Google Scholar]
  20. LANDS W. E. Metabolism of glycerolipids. 2. The enzymatic acylation of lysolecithin. J Biol Chem. 1960 Aug;235:2233–2237. [PubMed] [Google Scholar]
  21. Prottey C., Hawthorne J. N. The biosynthesis of phosphatidic acid and phosphatidylinositol in mammalian pancreas. Biochem J. 1967 Oct;105(1):379–392. doi: 10.1042/bj1050379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ROWE C. E. The biosynthesis of phospholipids by human blood cells. Biochem J. 1959 Nov;73:438–442. doi: 10.1042/bj0730438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scott T. W., Jay S. M., Freinkel N. Further studies on the action of pituitary thyrotropin on the individual phosphatides of thyroid tissue. Endocrinology. 1966 Sep;79(3):591–600. doi: 10.1210/endo-79-3-591. [DOI] [PubMed] [Google Scholar]
  24. Scott T. W., Setchell B. P., Bassett J. M. Characterization and metabolism of ovine foetal lipids. Biochem J. 1967 Sep;104(3):1040–1047. doi: 10.1042/bj1041040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Seljelid R. Endocytosis in thyroid follicle cells. V. On the redistribution of cytosomes following stimulation with thyrotropic hormone. J Ultrastruct Res. 1967 Jun;18(5):479–488. doi: 10.1016/s0022-5320(67)80198-9. [DOI] [PubMed] [Google Scholar]
  26. Wetzel B. K., Spicer S. S., Wollman S. H. Changes in fine structure and acid phosphatase localization in rat thyroid cells following thyrotropin administration. J Cell Biol. 1965 Jun;25(3):593–618. doi: 10.1083/jcb.25.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES