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. 1993 Dec;135(4):1091–1097. doi: 10.1093/genetics/135.4.1091

Nucleotide Polymorphism in the Adh1 Locus of Pearl Millet (Pennisetum Glaucum) (Poaceae)

B S Gaut 1, M T Clegg 1
PMCID: PMC1205740  PMID: 7905843

Abstract

We investigated nucleotide polymorphism in the Adh1 locus of pearl millet (Pennisetum glaucum) (Poaceae) by determining the DNA sequence of 20 alleles from 10 individuals. The individuals were sampled from throughout pearl millet's indigenous range and represent both wild and cultivated accessions. Our results indicated that there is little nucleotide polymorphism in the Adh1 locus. Estimates of per site nucleotide polymorphism did not differ significantly between cultivated and wild millet accessions. We compared nucleotide polymorphism in pearl millet Adh1 with nucleotide polymorphism in maize (Zea mays) Adh1 and conclude that the maize Adh1 sample is more polymorphic. Increased polymorphism in maize Adh1 may be attributable, in part, to faster substitution rates in the maize lineage. Analysis suggests that substitution rates in the maize Adh1 lineage are ~1.7 times faster than substitution rates in the millet Adh1 lineage.

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Selected References

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  1. Clark A. G. Inference of haplotypes from PCR-amplified samples of diploid populations. Mol Biol Evol. 1990 Mar;7(2):111–122. doi: 10.1093/oxfordjournals.molbev.a040591. [DOI] [PubMed] [Google Scholar]
  2. Dennis E. S., Gerlach W. L., Pryor A. J., Bennetzen J. L., Inglis A., Llewellyn D., Sachs M. M., Ferl R. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984 May 11;12(9):3983–4000. doi: 10.1093/nar/12.9.3983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Freeling M., Bennett D. C. Maize Adh1. Annu Rev Genet. 1985;19:297–323. doi: 10.1146/annurev.ge.19.120185.001501. [DOI] [PubMed] [Google Scholar]
  4. Fu Y. X., Li W. H. Statistical tests of neutrality of mutations. Genetics. 1993 Mar;133(3):693–709. doi: 10.1093/genetics/133.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gaut B. S., Clegg M. T. Molecular evolution of alcohol dehydrogenase 1 in members of the grass family. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2060–2064. doi: 10.1073/pnas.88.6.2060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gaut B. S., Clegg M. T. Molecular evolution of the Adh1 locus in the genus Zea. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5095–5099. doi: 10.1073/pnas.90.11.5095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gaut B. S., Muse S. V., Clark W. D., Clegg M. T. Relative rates of nucleotide substitution at the rbcL locus of monocotyledonous plants. J Mol Evol. 1992 Oct;35(4):292–303. doi: 10.1007/BF00161167. [DOI] [PubMed] [Google Scholar]
  8. Good A. G., Pelcher L. E., Crosby W. L. Nucleotide sequence of a complete barley alcohol dehydrogenase 1 cDNA. Nucleic Acids Res. 1988 Jul 25;16(14B):7182–7182. doi: 10.1093/nar/16.14.7182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johns M. A., Strommer J. N., Freeling M. Exceptionally High Levels of Restriction Site Polymorphism in DNA near the Maize Adh1 Gene. Genetics. 1983 Nov;105(3):733–743. doi: 10.1093/genetics/105.3.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kliman R. M., Hey J. DNA sequence variation at the period locus within and among species of the Drosophila melanogaster complex. Genetics. 1993 Feb;133(2):375–387. doi: 10.1093/genetics/133.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kreitman M., Hudson R. R. Inferring the evolutionary histories of the Adh and Adh-dup loci in Drosophila melanogaster from patterns of polymorphism and divergence. Genetics. 1991 Mar;127(3):565–582. doi: 10.1093/genetics/127.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Long M., Langley C. H. Natural selection and the origin of jingwei, a chimeric processed functional gene in Drosophila. Science. 1993 Apr 2;260(5104):91–95. doi: 10.1126/science.7682012. [DOI] [PubMed] [Google Scholar]
  13. MacRae A. F., Clegg M. T. Evolution of Ac and Dsl elements in select grasses (Poaceae). Genetica. 1992;86(1-3):55–66. doi: 10.1007/BF00133711. [DOI] [PubMed] [Google Scholar]
  14. McDonald J. H., Kreitman M. Adaptive protein evolution at the Adh locus in Drosophila. Nature. 1991 Jun 20;351(6328):652–654. doi: 10.1038/351652a0. [DOI] [PubMed] [Google Scholar]
  15. Muse S. V., Weir B. S. Testing for equality of evolutionary rates. Genetics. 1992 Sep;132(1):269–276. doi: 10.1093/genetics/132.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Osterman J. C., Dennis E. S. Molecular analysis of the ADH1-Cm allele of maize. Plant Mol Biol. 1989 Aug;13(2):203–212. doi: 10.1007/BF00016138. [DOI] [PubMed] [Google Scholar]
  17. Riley M. A., Kaplan S. R., Veuille M. Nucleotide polymorphism at the xanthine dehydrogenase locus in Drosophila pseudoobscura. Mol Biol Evol. 1992 Jan;9(1):56–69. doi: 10.1093/oxfordjournals.molbev.a040708. [DOI] [PubMed] [Google Scholar]
  18. Schaeffer S. W., Miller E. L. Nucleotide sequence analysis of Adh genes estimates the time of geographic isolation of the Bogota population of Drosophila pseudoobscura. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6097–6101. doi: 10.1073/pnas.88.14.6097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shattuck-Eidens D. M., Bell R. N., Neuhausen S. L., Helentjaris T. DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing. Genetics. 1990 Sep;126(1):207–217. doi: 10.1093/genetics/126.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Slatkin M., Maddison W. P. Detecting isolation by distance using phylogenies of genes. Genetics. 1990 Sep;126(1):249–260. doi: 10.1093/genetics/126.1.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tajima F. Evolutionary relationship of DNA sequences in finite populations. Genetics. 1983 Oct;105(2):437–460. doi: 10.1093/genetics/105.2.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989 Nov;123(3):585–595. doi: 10.1093/genetics/123.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Xie Y., Wu R. Rice alcohol dehydrogenase genes: anaerobic induction, organ specific expression and characterization of cDNA clones. Plant Mol Biol. 1989 Jul;13(1):53–68. doi: 10.1007/BF00027335. [DOI] [PubMed] [Google Scholar]

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