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. 1965 Aug;96(2):500–506. doi: 10.1042/bj0960500

A comparison of methods for the isolation and fractionation of reticulocyte ribosomes

H R V Arnstein 1, R A Cox 1, H Gould 1, H Potter 1
PMCID: PMC1207066  PMID: 4953780

Abstract

1. Polysomes, ribosomes and pH5 enzymes were isolated from rabbit reticulocytes by acidifying the post-mitochondrial supernatant to pH6·0 to precipitate all ribonucleoprotein particles and about half the pH5 enzymes; the precipitate was redissolved in buffer, pH7·6, and fractionated by zone centrifuging. 2. The isolation of polysome-rich and ribosome-rich fractions from the post-mitochondrial supernatant was also examined. 3. Studies of the stability of polysomes revealed that dissociation into sub-units occurred when both bound and free Mg2+ was chelated by EDTA or when the pH was increased above pH8·8.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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