Abstract
The genomic subtraction method representational difference analysis (RDA) was used to identify male-specific restriction fragments in the dioecious plant Silene latifolia. Male-specific restriction fragments are linked to the male sex chromosome (the Y chromosome). Four RDA-derived male-specific restriction fragments were used to identify polymorphisms in a collection of X-ray-generated mutant plants with either hermaphroditic or asexual flowers. Some of the mutants have cytologically detectable deletions in the Y chromosome that were correlated with loss of male-specific restriction fragments. One RDA-derived probe detected a restriction fragment present in all mutants, indicating that each has retained Y chromosomal DNA. The other three probes detected genomic fragments that were linked in a region deleted in some hermaphroditic and some asexual mutants. Based on the mutant phenotypes and the correlation of cytologically visible deletions with loss of male-specific restriction fragments, these markers were assigned to positions on the Y chromosome close to the carpel suppression locus. This RDA mapping also revealed a Y-linked locus, not previously described, which is responsible for early stamen development.
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Selected References
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- Dellaporta S. L., Calderon-Urrea A. Sex determination in flowering plants. Plant Cell. 1993 Oct;5(10):1241–1251. doi: 10.1105/tpc.5.10.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lisitsyn N., Lisitsyn N., Wigler M. Cloning the differences between two complex genomes. Science. 1993 Feb 12;259(5097):946–951. doi: 10.1126/science.8438152. [DOI] [PubMed] [Google Scholar]
- Shure M., Wessler S., Fedoroff N. Molecular identification and isolation of the Waxy locus in maize. Cell. 1983 Nov;35(1):225–233. doi: 10.1016/0092-8674(83)90225-8. [DOI] [PubMed] [Google Scholar]
- Stadler L. J., Roman H. The Effect of X-Rays upon Mutation of the Gene A in Maize. Genetics. 1948 May;33(3):273–303. doi: 10.1093/genetics/33.3.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor D. R. The genetic basis of sex ratio in Silene alba (= S. latifolia). Genetics. 1994 Feb;136(2):641–651. doi: 10.1093/genetics/136.2.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WESTERGAARD M. The mechanism of sex determination in dioecious flowering plants. Adv Genet. 1958;9:217–281. doi: 10.1016/s0065-2660(08)60163-7. [DOI] [PubMed] [Google Scholar]
- Warmke H. E., Blakeslee A. F. SEX MECHANISM IN POLYPLOIDS OF MELANDRIUM. Science. 1939 Apr 28;89(2313):391–392. doi: 10.1126/science.89.2313.391. [DOI] [PubMed] [Google Scholar]