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. 1977 Jul;123(Pt 3):783–796.

A study of mammalian intrafusal muscle fibres using a combined histochemical and ultrastructural technique.

R W Banks, D W Harker, M J Stacey
PMCID: PMC1234735  PMID: 142074

Abstract

A direct correlation of the histochemical and ultrastructural properties of intrafusal muscle fibres has been achieved by cutting frozen serial sections for histochemical applications (15 micron thick sections) and for electron microscopy (60 micron thick sections) in a repeating sequence. Three types of intrafusal fibre were recognized, including two types of nuclear-bag fibre, designated bag1 and bag2. In addition to histochemical and ultrastructural differences, the three types of fibre differed in length and diameter. Regional variations of histochemical and ultrastructural properties were found. The results are compared with previous attempts to correlate histochemical and ultrastructural properties of intrafusal muscle fibres based on indirect methods.

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Selected References

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  1. Arendt K. W., Asmussen G. Enzymhistochemische Untersuchungen an Muskelspindeln verschiedener Spezies. Anat Anz. 1974;136(3):217–228. [PubMed] [Google Scholar]
  2. BARKER D., GIDUMAL J. L. The morphology of intrafusal muscle fibres in the cat. J Physiol. 1961 Aug;157:513–528. doi: 10.1113/jphysiol.1961.sp006740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker D., Stacey M. J. Rabbit intrafusal muscle fibres. J Physiol. 1970 Sep;210(2):70P–72P. [PubMed] [Google Scholar]
  4. Banker B. Q., Girvin J. P. The ultrastructural features of the mammalian muscle spindle. J Neuropathol Exp Neurol. 1971 Apr;30(2):155–195. doi: 10.1097/00005072-197104000-00001. [DOI] [PubMed] [Google Scholar]
  5. Banks R. W., Barker D., Harker D. W., Stacey M. J. Proceedings Correlation between ultrastructure and histochemistry of mammalian intrafusal muscle fibres. J Physiol. 1975 Nov;252(2):16P–17P. [PMC free article] [PubMed] [Google Scholar]
  6. Banks R. W., James N. T. The fine structure of the guinea-pig muscle spindle. Z Zellforsch Mikrosk Anat. 1973;140(3):357–368. doi: 10.1007/BF00307023. [DOI] [PubMed] [Google Scholar]
  7. Barker D., Laporte Y. Proceedings of the anatomical society of great britain and ireland, april 1974: symposium on muscle spindles. J Anat. 1975 Feb;119(Pt 1):183–207. [PMC free article] [PubMed] [Google Scholar]
  8. ERANKO O., PALKAMA A. Improved localization of phosphorylase by the use of polyvinyl pyrrolidone and high substrate concentration. J Histochem Cytochem. 1961 Sep;9:585–585. doi: 10.1177/9.5.585. [DOI] [PubMed] [Google Scholar]
  9. Guth L., Samaha F. J. Procedure for the histochemical demonstration of actomyosin ATPase. Exp Neurol. 1970 Aug;28(2):365–367. [PubMed] [Google Scholar]
  10. James N. T. The histochemical demonstration of myoglobin in muscle spindles. Histochem J. 1971 Sep;3(5):333–338. doi: 10.1007/BF01005015. [DOI] [PubMed] [Google Scholar]
  11. James N. T. The histochemical demonstration of three types of intrafusal fibre in rat muscle spindles. Histochem J. 1971 Nov;3(6):457–462. doi: 10.1007/BF01014784. [DOI] [PubMed] [Google Scholar]
  12. Milburn A. The early development of muscle spindles in the rat. J Cell Sci. 1973 Jan;12(1):175–195. doi: 10.1242/jcs.12.1.175. [DOI] [PubMed] [Google Scholar]
  13. OGATA T., MORI M. HISTOCHEMICAL STUDY OF OXIDATIVE ENZYMES IN VERTEBRATE MUSCLES. J Histochem Cytochem. 1964 Mar;12:171–182. doi: 10.1177/12.3.171. [DOI] [PubMed] [Google Scholar]
  14. Ovalle W. K., Jr Fine structure of rat intrafusal muscle fibers. The polar region. J Cell Biol. 1971 Oct;51(1):83–103. doi: 10.1083/jcb.51.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ovalle W. K., Smith R. S. Histochemical identification of three types of intrafusal muscle fibers in the cat and monkey based on the myosin ATPase reaction. Can J Physiol Pharmacol. 1972 Mar;50(3):195–202. doi: 10.1139/y72-030. [DOI] [PubMed] [Google Scholar]
  16. Scalzi H. A., Price H. M. The arrangement and sensory innervation of the intrafusal fibers in the feline muscle spindle. J Ultrastruct Res. 1971 Aug;36(3):375–390. doi: 10.1016/s0022-5320(71)80111-9. [DOI] [PubMed] [Google Scholar]
  17. Schiaffino S., Hanzlíková V., Pierobon S. Relations between structure and function in rat skeletal muscle fibers. J Cell Biol. 1970 Oct;47(1):107–119. doi: 10.1083/jcb.47.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WIRSEN C., LARSSON K. S. HISTOCHEMICAL DIFFERENTIATION OF SKELETAL MUSCLE IN FOETAL AND NEWBORN MICE. J Embryol Exp Morphol. 1964 Dec;12:759–767. [PubMed] [Google Scholar]
  19. Yellin H. A histochemical study of muscle spindles and their relationship to extrafusal fiber types in the rat. Am J Anat. 1969 May;125(1):31–45. doi: 10.1002/aja.1001250103. [DOI] [PubMed] [Google Scholar]
  20. Yellin H. Regional differences in the contractile apparatus of intrafusal muscle fibers. Am J Anat. 1974 Jan;139(1):147–152. doi: 10.1002/aja.1001390111. [DOI] [PubMed] [Google Scholar]

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