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. 1966 Mar;98(3):754–762. doi: 10.1042/bj0980754

The choline acetyltransferase of human placenta

D Morris 1
PMCID: PMC1264916  PMID: 5911523

Abstract

1. Various methods for the extraction of choline acetyltransferase (acetyl-CoA–choline O-acetyltransferase, EC 2.3.1.6) from immature human placenta (18–28 weeks of gestation) are described. 2. The crude enzyme was found to be stable at −18° and +4° under a variety of conditions. 3. Purification methods, including ammonium sulphate fractionation, gel filtration on various grades of Sephadex and DEAE-Sephadex fractionation, have yielded a preparation of high specific activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BULL G., FEINSTEIN A., MORRIS D. SEDIMENTATION BEHAVIOR AND MOLECULAR WEIGHT OF CHOLINE ACETYLTRANSFERASE. Nature. 1964 Mar 28;201:1326–1326. doi: 10.1038/2011326a0. [DOI] [PubMed] [Google Scholar]
  2. BURGEN A. S., BURKE G., DESBARATSSCHONBAUM M. L. The specificity of brain choline acetylase. Br J Pharmacol Chemother. 1956 Sep;11(3):308–312. doi: 10.1111/j.1476-5381.1956.tb01071.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang H. C., Gaddum J. H. Choline esters in tissue extracts. J Physiol. 1933 Oct 6;79(3):255–285. doi: 10.1113/jphysiol.1933.sp003049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HEBB C. O. Biochemical evidence for the neural function of acetylcholine. Physiol Rev. 1957 Apr;37(2):196–220. doi: 10.1152/physrev.1957.37.2.196. [DOI] [PubMed] [Google Scholar]
  5. HEBB C. O., KRNJEVIC K., SILVER A. ACETYLCHOLINE AND CHOLINE ACETYLTRANSFERASE IN THE DIAPHRAGM OF THE RAT. J Physiol. 1964 Jun;171:504–513. doi: 10.1113/jphysiol.1964.sp007393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HEMSWORTH B. A., MORRIS D. A COMPARISON OF THE N-ALKYL GROUP SPECIFICITY OF CHOLINE ACETYLTRANSFERASE FROM DIFFERENT SPECIES. J Neurochem. 1964 Nov;11:793–803. doi: 10.1111/j.1471-4159.1964.tb06728.x. [DOI] [PubMed] [Google Scholar]
  7. Hebb C. O., Ratković D. Choline acetylase in the placenta of man and other species. J Physiol. 1962 Sep;163(2):307–313. doi: 10.1113/jphysiol.1962.sp006976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KUMAGAI H., EBASHI S. Highly purified choline acetylase. Nature. 1954 May 8;173(4410):871–872. doi: 10.1038/173871a0. [DOI] [PubMed] [Google Scholar]
  9. NORDENFELT I. Choline acetylase in normal and denervated salivary glands. Q J Exp Physiol Cogn Med Sci. 1963 Jan;48:67–79. doi: 10.1113/expphysiol.1963.sp001639. [DOI] [PubMed] [Google Scholar]
  10. REISBERG R. B. Properties and biological significance of choline acetylase. Yale J Biol Med. 1957 Feb;29(4):403–435. [PMC free article] [PubMed] [Google Scholar]
  11. SIEGEL L. M., MONTY K. J. DETERMINATION OF MOLECULAR WEIGHTS AND FRICTIONAL RATIOS OF MACROMOLECULES IN IMPURE SYSTEMS: AGGREGATION OF UREASE. Biochem Biophys Res Commun. 1965 May 3;19:494–499. doi: 10.1016/0006-291x(65)90152-x. [DOI] [PubMed] [Google Scholar]
  12. SMALLMAN B. N. Mechanisms of acetylcholine synthesis in the blowfly. J Physiol. 1956 May 28;132(2):343–357. doi: 10.1113/jphysiol.1956.sp005527. [DOI] [PMC free article] [PubMed] [Google Scholar]

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