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. 1966 Jun;99(3):703–707. doi: 10.1042/bj0990703

Inhibitory effect of salicylate on the incorporation of l-[U-14C]leucine into the protein of rat tissue preparations in vitro

P D Dawkins 1, B J Gould 1, M J H Smith 1
PMCID: PMC1265061  PMID: 5964967

Abstract

1. The inhibitory effect of salicylate, in concentrations ranging from 0·1 to 20mm, on the incorporation of radioactivity from l-[U-14C]leucine into the protein of isolated rat diaphragm muscle and of cell-free systems from rat liver was studied. 2. The lowest salicylate concentrations producing significant inhibitions of amino acid incorporation were as follows: isolated rat diaphragm, 0·1mm; rat-liver mitochondrial–microsomal system, 0·1mm; rat-liver microsomal system, 0·3mm. 3. Salicylate concentrations of 2·5mm and above were found to inhibit creatine-kinase activity in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDREWS B. F., BRUTON O. C., DE BAARE L. Serum amino acid nitrogen in infancy and childhood. J Pediatr. 1962 Feb;60:201–205. doi: 10.1016/s0022-3476(62)80037-7. [DOI] [PubMed] [Google Scholar]
  2. BRODY T. M. Action of sodium salicylate and related compounds on tissue metabolism in vitro. J Pharmacol Exp Ther. 1956 May;117(1):39–51. [PubMed] [Google Scholar]
  3. Eggleton P., Elsden S. R., Gough N. The estimation of creatine and of diacetyl. Biochem J. 1943;37(5):526–529. doi: 10.1042/bj0370526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MANCHESTER K. L., RANDLE P. J., SMITH G. H. Some effects of sodium salicylate on muscle metabolism. Br Med J. 1958 May 3;1(5078):1028–1030. doi: 10.1136/bmj.1.5078.1028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. SEGAL S., BLAIR A. IN VITRO EFFECT OF SALICYLATE ON AMINO ACID ACCUMULATION BY KIDNEY CORTEX SLICES. Nature. 1963 Oct 12;200:139–141. doi: 10.1038/200139a0. [DOI] [PubMed] [Google Scholar]
  6. TATA J. R., ERNSTER L., LINDBERG O., ARRHENIUS E., PEDERSEN S., HEDMAN R. The action of thyroid hormones at the cell level. Biochem J. 1963 Mar;86:408–428. doi: 10.1042/bj0860408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. WEISS W. P., CAMPBELL P. L., DEIBLER G. E., SOKOLOFF L. Effects of salicylate on amino acid incorporation into protein. J Pharmacol Exp Ther. 1962 Jun;136:366–371. [PubMed] [Google Scholar]
  8. WINTERS R. W., MORRILL M. F. Carbohydrate metabolism in experimental salicylism. Proc Soc Exp Biol Med. 1955 Mar;88(3):409–411. doi: 10.3181/00379727-88-21602. [DOI] [PubMed] [Google Scholar]

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