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. 1966 Sep;100(3):671–674. doi: 10.1042/bj1000671

Inhibition of purine phosphoribosyltransferases from Ehrlich ascites-tumour cells by purine nucleotides

A W Murray 1
PMCID: PMC1265200  PMID: 5969281

Abstract

1. The purine bases adenine, hypoxanthine and guanine were rapidly incorporated into the nucleotide fraction of Ehrlich ascites-tumour cells in vivo. 2. The reaction of 5′-phosphoribosyl pyrophosphate with adenine phosphoribosyltransferase from ascites-tumour cells (Km 6·5–11·9μm) was competitively inhibited by AMP, ADP, ATP and GMP (Ki 7·5, 21·9, 395 and 118μm respectively). Similarly the reactions of 5′-phosphoribosyl pyrophosphate with both hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase (Km 18·4–31 and 37·6–44·2μm respectively) were competitively inhibited by IMP (Ki 52 and 63·5μm) and by GMP (Ki 36·5 and 5·9μm). 3. The nucleotides tested as inhibitors did not appreciably compete with the purine bases in the phosphoribosyltransferase reactions. 4. It was postulated that the purine phosphoribosyltransferases of Ehrlich ascites-tumour cells may be effectively separated from the adenine nucleotide pool of these cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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