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. 1966 Sep;100(3):671–674. doi: 10.1042/bj1000671

Inhibition of purine phosphoribosyltransferases from Ehrlich ascites-tumour cells by purine nucleotides

A W Murray 1
PMCID: PMC1265200  PMID: 5969281

Abstract

1. The purine bases adenine, hypoxanthine and guanine were rapidly incorporated into the nucleotide fraction of Ehrlich ascites-tumour cells in vivo. 2. The reaction of 5′-phosphoribosyl pyrophosphate with adenine phosphoribosyltransferase from ascites-tumour cells (Km 6·5–11·9μm) was competitively inhibited by AMP, ADP, ATP and GMP (Ki 7·5, 21·9, 395 and 118μm respectively). Similarly the reactions of 5′-phosphoribosyl pyrophosphate with both hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase (Km 18·4–31 and 37·6–44·2μm respectively) were competitively inhibited by IMP (Ki 52 and 63·5μm) and by GMP (Ki 36·5 and 5·9μm). 3. The nucleotides tested as inhibitors did not appreciably compete with the purine bases in the phosphoribosyltransferase reactions. 4. It was postulated that the purine phosphoribosyltransferases of Ehrlich ascites-tumour cells may be effectively separated from the adenine nucleotide pool of these cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATKINSON M. R., MORTON R. K., MURRAY A. W. INHIBITION OF INOSINE 5'-PHOSPHATE DEHYDROGENASE FROM EHRLICH ASCITES-TUMOUR CELLS BY 6-THIONINOSINE 5'-PHOSPHATE. Biochem J. 1963 Oct;89:167–172. doi: 10.1042/bj0890167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ATKINSON M. R., MURRAY A. W. INHIBITION BY 6-MERCAPTOPURINE OF PURINE PHOSPHORIBOSYLTRANSFERASES FROM EHRLICH ASCITES-TUMOUR CELLS THAT ARE RESISTANT TO THE DRUG. Biochem J. 1965 Jan;94:71–74. doi: 10.1042/bj0940071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ATKINSON M. R., MURRAY A. W. INHIBITION OF PRUINE PHOSPHORIBOSYLTRANSFERASES OF EHRLICH ASCITES-TUMOUR CELLS BY 6-MERCAPTOPURINE. Biochem J. 1965 Jan;94:64–70. doi: 10.1042/bj0940064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HENDERSON J. F., KHOO M. K. AVAILABILITY OF 5-PHOSPHORIBOSYL 1-PYROPHOSPHATE FOR RIBONUCLEOTIDE SYNTHESIS IN EHRLICH ASCITES TUMOR CELLS IN VITRO. J Biol Chem. 1965 Jun;240:2358–2362. [PubMed] [Google Scholar]
  5. HENDERSON J. F., KHOO M. K. SYNTHESIS OF 5-PHOSPHORIBOSYL 1-PYROPHOSPHATE FROM GLUCOSE IN EHRLICH ASCITES TUMOR CELLS IN VITRO. J Biol Chem. 1965 Jun;240:2349–2357. [PubMed] [Google Scholar]
  6. KORNBERG A., LIEBERMAN I., SIMMS E. S. Enzymatic synthesis and properties of 5-phosphoribosylpyrophosphate. J Biol Chem. 1955 Jul;215(1):389–402. [PubMed] [Google Scholar]
  7. MAGER J., MAGASANIK B. Guanosine 5'-phosphate reductase and its role in the interconversion of purine nucleotides. J Biol Chem. 1960 May;235:1474–1478. [PubMed] [Google Scholar]
  8. Murray A. W. Purine-phosphoribosyltransferase activities in rat and mouse tissues and in Ehrlich ascites-tumour cells. Biochem J. 1966 Sep;100(3):664–670. doi: 10.1042/bj1000664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NIERLICH D. P., MAGASANIK B. REGULATION OF PURINE RIBONUCLEOTIDE SYNTHESIS BY END PRODUCT INHIBITION. THE EFFECT OF ADENINE AND GUANINE RIBONUCLEOTIDES ON THE 5'-PHOSPHORIBOSYL-PYROPHOSPHATE AMIDOTRANSFERASE OF AEROBACTER AEROGENES. J Biol Chem. 1965 Jan;240:358–365. [PubMed] [Google Scholar]
  10. Overgaard-Hansen K. Metabolic regulation of the adenine nucleotide pool. I. Studies on the transient exhaustion of the adenine nucleotides by glucose in Ehrlich ascites tumor cells. Biochim Biophys Acta. 1965 Jul 8;104(2):330–347. [PubMed] [Google Scholar]
  11. PATERSON A. R., HORI A. Resistance to 6-mercaptopurine. I. Biochemical differences between the Ehrlich ascites carcinoma and a 6-mercaptopurine-resistant subline. Can J Biochem Physiol. 1962 Feb;40:181–194. [PubMed] [Google Scholar]
  12. WYNGAARDEN J. B., GREENLAND R. A. The inhibition of succinoadenylate kinosynthetase of Escherichia coli by adenosine and guanosine 5'-monophosphates. J Biol Chem. 1963 Mar;238:1054–1057. [PubMed] [Google Scholar]

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