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. 1966 Sep;100(3):739–744. doi: 10.1042/bj1000739

Kinetic studies with skeletal-muscle hexokinase

C J Toews 1
PMCID: PMC1265209  PMID: 16742416

Abstract

Rat skeletal-muscle hexokinase was partially purified by ammonium sulphate fractionation and gel filtration. The mechanism of the skeletal-muscle hexokinase was studied kinetically by initial-velocity analysis and product inhibition. Glucose 6-phosphate was a non-competitive inhibitor of glucose and ATP. ADP was a non-competitive inhibitor of glucose and a competitive inhibitor of ATP. The data on product inhibition and initial-velocity analysis of skeletal-muscle hexokinase support an ordered sequential mechanism (ordered Bi Bi) where the addition of substrates and release of products is in the order: ATP, glucose, glucose 6-phosphate and ADP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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