Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1966 Sep;100(3):739–744. doi: 10.1042/bj1000739

Kinetic studies with skeletal-muscle hexokinase

C J Toews 1
PMCID: PMC1265209  PMID: 16742416

Abstract

Rat skeletal-muscle hexokinase was partially purified by ammonium sulphate fractionation and gel filtration. The mechanism of the skeletal-muscle hexokinase was studied kinetically by initial-velocity analysis and product inhibition. Glucose 6-phosphate was a non-competitive inhibitor of glucose and ATP. ADP was a non-competitive inhibitor of glucose and a competitive inhibitor of ATP. The data on product inhibition and initial-velocity analysis of skeletal-muscle hexokinase support an ordered sequential mechanism (ordered Bi Bi) where the addition of substrates and release of products is in the order: ATP, glucose, glucose 6-phosphate and ADP.

Full text

PDF
743

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLELAND W. W. The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations. Biochim Biophys Acta. 1963 Jan 8;67:104–137. doi: 10.1016/0006-3002(63)91800-6. [DOI] [PubMed] [Google Scholar]
  2. CLELAND W. W. The kinetics of enzyme-catalyzed reactions with two or more substrates or products. II. Inhibition: nomenclature and theory. Biochim Biophys Acta. 1963 Feb 12;67:173–187. doi: 10.1016/0006-3002(63)91815-8. [DOI] [PubMed] [Google Scholar]
  3. CRANE R. K., SOLS A. The non-competitive inhibition of brain hexokinase by glucose-6-phosphate and related compounds. J Biol Chem. 1954 Oct;210(2):597–606. [PubMed] [Google Scholar]
  4. FRIEDEN C. Glutamic dehydrogenase. III. The order of substrate addition in the enzymatic reaction. J Biol Chem. 1959 Nov;234:2891–2896. [PubMed] [Google Scholar]
  5. FROMM H. J., SILVERSTEIN E., BOYER P. D. EQUILIBRIUM AND NET REACTION RATES IN RELATION TO THE MECHANISM OF YEAST HEXOKINASE. J Biol Chem. 1964 Nov;239:3645–3652. [PubMed] [Google Scholar]
  6. FROMM H. J., ZEWE V. Kinetic studies of the brain hexokinase reaction. J Biol Chem. 1962 May;237:1661–1667. [PubMed] [Google Scholar]
  7. FROMM H. J., ZEWE V. Kinetic studies of yeast hexokinase. J Biol Chem. 1962 Oct;237:3027–3032. [PubMed] [Google Scholar]
  8. Hanson T. L., Fromm H. J. Rat skeletal muscle hexokinase. I. Kinetics and reaction mechanism. J Biol Chem. 1965 Nov;240(11):4133–4139. [PubMed] [Google Scholar]
  9. KERLY M., LEABACK D. H. The characteristics of hexokinase from Locusta migratoria muscle. Biochem J. 1957 Oct;67(2):245–250. doi: 10.1042/bj0670245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LIEBECQ C. The hexokinase activity of rat muscle extract and the lability of the Mg-ATP complex. Biochem J. 1953 Jun;54(3):xxii–xxiii. [PubMed] [Google Scholar]
  11. NOVOA W. B., WINER A. D., GLAID A. J., SCHWERT G. W. Lactic dehydrogenase. V. Inhibition by oxamate and by oxalate. J Biol Chem. 1959 May;234(5):1143–1148. [PubMed] [Google Scholar]
  12. SALAS J., SALAS M., VINUELA E., SOLS A. GLUCOKINASE OF RABBIT LIVER. J Biol Chem. 1965 Mar;240:1014–1018. [PubMed] [Google Scholar]
  13. SLEIN M. W., CORI G. T., CORI C. F. A comparative study of hexokinase from yeast and animal tissues. J Biol Chem. 1950 Oct;186(2):763–780. [PubMed] [Google Scholar]
  14. WEIL-MALHERBE H., BONE A. D. Studies on hexokinase. 1. The hexokinase activity of rat-brain extracts. Biochem J. 1951 Aug;49(3):339–347. doi: 10.1042/bj0490339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WRATTEN C. C., CLELAND W. W. PRODUCT INHIBITION STUDIES ON YEAST AND LIVER ALCOHOL DEHYDROGENASES. Biochemistry. 1963 Sep-Oct;2:935–941. doi: 10.1021/bi00905a007. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES